The traditional wrought Al–Mg–Si alloys fabricated via laser powder bed fusion (LPBF) are prone to hot cracks, unless adding grain refiners in as-LPBFed Al alloys. In this work, the Al-9.6 wt.%Mg-4.9 wt.%Si (equivalent pseudo binary eutectic Al-13.3 wt.%Mg2Si) alloy with low solidification range and hot-cracking susceptibility was successfully processed by LPBF. have reached a high relative density of 99.3% at VED 129.6 J/mm3. microstructures were featured fine α-Al grains cellular Mg2Si,...

An accurate in vitro model of human adipose tissue could assist the study adipocyte function and allow for better tools screening new therapeutic compounds. Cell culture models on two-dimensional surfaces fall short mimicking three-dimensional vivo environment, while are often unable to support long-term cell due, part, insufficient mass transport. Microfluidic systems have been explored models. However, current primarily focused 2D cultured adipocytes. In this work, a 3D microtissue was...

Microstructural evolution and mechanical properties of H13 steel fabricated by selective laser melting (SLM) with subsequent tempering treatment were systematically examined. It was found that the microstructure as-SLMed samples consisted cellular structures, lath martensite high-volume fraction retained austenite. After at 600 °C for 1 h, nanoscale Cr23C6 particles detected boundaries partially dissolved structures. The fine grains, formation carbides significantly improved steel. A...

Non-parenteral drug delivery systems using biomaterials have advantages over traditional parenteral strategies. For ocular and intranasal delivery, nanoparticulate must bind to permeate through mucosal epithelium other biological barriers. The incorporation of mucoadhesive permeation-enhancing such as chitosan facilitate this, but tend increase the size polydispersity nanoparticles, making practical optimization implementation nanoparticle formulations a challenge. In this study, we adjusted...

H13 stool steel processed by selective laser melting (SLM) suffered from severe brittleness and scatter distribution of mechanical properties. We optimized the response as-SLMed tailoring optimisation process parameters established correlation between microstructure properties in this work. Microstructures were examined using XRD, SEM, EBSD TEM. The results showed that microstructures predominantly featured cellular structures columnar grains, which consisted lath martensite retained...

Abstract Top-down functionalization of nanoparticles with cellular membranes imparts enhanced bio-interfacing capabilities. Initial methods for membrane coating involved physical co-extrusion and vesicles through a porous membrane; however, recent works employ sonication as the disruptive force to reform around surface nanoparticles. Although is widely used, there remains paucity information on effects variables efficiency, leading inconsistent across studies. In this work, we present...

The high Fe-containing AlSi12 alloy was processed by additive manufacturing of laser powder bed fusion (LPBF) to understand the features microstructures and mechanical properties under as-fabricated condition. Fe impurity found be beneficial for property enhancement in LPBFed samples. parameters including combination power 200 W, scanning speed 1110 mm/s, hatch spacing 0.15 mm, layer thickness 0.03 mm volumetric energy density 40 J/mm3 were optimized achieve a relative 99.7%. as-LPBFed...

In vitro adipose tissue models can be used to provide insight into fundamental aspects of physiology. These systems may serve as replacements for animal models, which are often poor predictors obesity and metabolic diseases in humans. Adipose consists a rich vasculature that is essential its function. However, the study endothelial cell–adipocyte interactions has been challenging due differences culture conditions required survival function each cell type. To address this issue, we performed...

Laminin α4 (LAMA4) is located in the extracellular basement membrane that surrounds each individual adipocyte. Here we show LAMA4 null (Lama4−/−) mice exhibit significantly higher energy expenditure (EE) relative to wild-type (WT) at room temperature and when exposed a cold challenge, despite similar levels of food intake locomotor activity. The Lama4−/− are resistant age- diet-induced obesity. Expression uncoupling protein 1 subcutaneous white adipose tissue WT animals on either chow diet...

Brown and beige adipose tissues have a significant capacity for energy expenditure that may be exploited as treatment obesity metabolic disease.

Abstract Better experimental models are needed to enhance our understanding of metabolic regulation which is seen in obesity and disorders, such as type 2 diabetes. In vitro based on microfluidics enable physiological representations tissues with several advantages over conventional culture systems, perfused flow better mimic the environment. Although cell lines 3T3‐L1 have been incorporated microfluidic devices, murine primary preadipocytes not differentiated maintained for long‐term...

Peptide nucleic acids (PNAs) are a class of acid mimics that can bind to the complementary DNA or RNA with high specificity and sensitivity. PNA-based microarrays have distinct characteristics improved performance in many aspects compared microarrays. A new set PNA monomers has been synthesized used as building blocks for preparation density These their backbones protected by photolabile group 2-(2-nitrophenyl)propyloxy carbonyl (NPPOC), exocyclic amino groups amide groups. light-directed...

Abstract Gradients in mechanical properties, physical architecture and biochemical composition exist a variety of complex tissues, yet 3D vitro models that enable investigation these cues on cellular processes, especially those contributing to vascularization engineered tissues are limited. Here, photopolymerization approach create cell‐laden hydrogel biomaterials with decoupled combined gradients modulus, immobilized cell adhesive peptide (RGD) concentration, proteolytic degradation...

Vascularization is critical for engineering mineralized tissues. It has been previously shown that biomaterials containing preformed endothelial networks anastomose to host vasculature following implantation. However, the alone may not increase regeneration. In addition, a clinically applicable source of cells vascularization needed. this study, vascular were generated from (ECs) derived human induced pluripotent stem (iPSCs). Network formation by iPSC-ECs within fibrin gels was investigated...

In the present study, densification behavior, microstructural evolution, mechanical properties, and friction behavior of a TiB2/Al8SiCu composite Al8SiCu alloy manufactured by laser powder-bed fusion (PBF-LB) were systematically investigated. The results confirm that addition in situ TiB2 particles into alloys reduce volumetric energy density required for high-density composite. promoted transformation columnar to equiaxed crystals formation high-angle grain boundaries. grains on vertical...