Background: Although substantial efforts have been made to link the gut microbiota type 2 diabetes, dynamic changes in fecal microbiome under pathological conditions of diabetes not investigated. Methods: Four male Zucker diabetic fatty (ZDF) rats received Purina 5008 chow [protein = 23.6%, Nitrogen-Free Extract (by difference) 50.3%, fiber (crude) 3.3%, ash 6.1%, fat (ether extract) 6.7%, and (acid hydrolysis) 8.1%] for 8 weeks. A total 32 stool samples were collected from weeks 15 four...

Obesity is a chronic metabolic disease caused by genetic and environmental factors that has become serious global health problem. There evidence gut microbiota closely related to the occurrence development of obesity. Erchen Decoction (ECD), traditional Chinese medicine, been widely used for clinical treatment basic research obesity diseases in recent years. It can significantly improve insulin resistance (IR) lipid metabolism disorders. However, there no microbiological study on its...

Gut microbiota is becoming one of the key determinants in human health and disease. Shifts gut composition affect cognitive function provide new insights for prevention treatment neurological diseases. Diabetes-associated decline (DACD) central nervous system complications type 2 diabetes mellitus (T2DM). ZiBuPiYin recipe (ZBPYR), a traditional Chinese medicine (TCM) formula, has long been used T2DM DACD. However, contribution ZBPYR to interaction between metabolism preventing treating DACD...

The incidence of obesity is increasing worldwide. It was reported that endoplasmic reticulum stress (ERS) could inhibit insulin receptor signaling by activating c-Jun N-terminal kinase (JNK) in the liver. However, relationship between ERS and brain during remains unclear. aim current study to assess whether alters through hyper-activation JNK hippocampus frontal cortex brains obese rats. Obesity induced using a high fat diet (HFD). Morris water maze test then performed evaluate decreases...

Multiple organ systems, including the brain, which undergoes changes that may increase risk of cognitive decline, are adversely affected by diabetes mellitus (DM). Here, we demonstrate type 2 (T2DM) db/db mice exhibited hippocampus-dependent memory impairment, might associate with a reduction in dendritic spine density pyramidal neurons Aβ1-42 deposition prefrontal cortex (PFC) and hippocampus, decreased expression neurostructural proteins microtubule-associated protein (MAP2), marker...

Cognitive impairment is a complication of type 2 diabetes mellitus (T2DM) that affects the central nervous system (CNS). Studies have shown chronic psychological stress may promote development T2DM into diabetes-associated cognitive decline (DACD). Previously, in was correlated predominantly with insulin resistance medial prefrontal cortex (mPFC).We examined effect ZiBuPiYin recipe (ZBPYR) on Zucker diabetic fatty (ZDF) rats and explored impact altered β-amyloid (Aβ) metabolism through...

Obesity is one of the susceptibility factors for type 2 diabetes (T2DM), both which could accelerate aging body and bring many hazards. A causal relationship present between intestinal microbiota metabolism, but how play a role in progression obesity to T2DM has not been elucidated. In this study, we transplanted healthy or obese-T2DM ZDF LZ rats, used 16S rRNA targeted metabonomics evaluate directional effect on obese rats T2DM. The glycolipid metabolism phenotype be changed bidirectionally...

The Zibu Piyin Recipe (ZBPYR) is derived from Zicheng Decoction, a traditional Chinese medicine formula recorded in the book of Bujuji, written by Wu Cheng Qing dynasty and used for clinical treatment amnesia. Our aim was to study effects fractions on scopolamine-induced learning memory impairment mouse. Crude extracts were prepared using various solvents, individual produced following D101 macroporous resin column chromatography. passive avoidance task, step down test Morris water maze then...

To identify circulating micro (mi)RNAs as biological markers for prediction of severe acute pancreatitis (SAP) with lung injury (ALI).Twenty-four serum samples were respectively collected and classified SAP associated ALI without ALI, the miRNA expression profiles determined by microarray analysis. These miRNAs validated quantitative reverse transcription-polymerase chain reaction, their putative targets predicted online software TargetScan, miRanda PicTar database. Gene ontology (GO) Kyoto...

Diabetes related cognitive dysfunction (DACD), one of the chronic complications diabetes, seriously affect quality life in patients and increase family burden. Although initial stage DACD can lead to metabolic alterations or potential pathological changes, is difficult diagnose accurately. Moreover, details molecular mechanism remain somewhat elusive. To understand pathophysiological changes that underpin development progression DACD, we carried out a global analysis response DACD. The...

Dai-Huang-Fu-Zi-Tang (DHFZT) is a famous traditional Chinese prescription with intestinal obstruction, acute pancreatitis and cholecystalgia for thousands of years. Our previous work found that DHFZT could act against pulmonary pathological injury in rats severe (SAP). But the underlying mechanism has not been fully elucidated. The aim present study was to investigate whether relieve by regulating aquaporins after SAP induced sodium taurocholate rats. Forty SD were used dose dependant...

Disturbance in energy metabolism, as a key factor diabetes-associated cognitive decline (DACD), has become promising therapeutic target of Chinese medicine ZiBu PiYin Recipe (ZBPYR). However, it is still not clear how ZBPYR affects the mitochondrial function DACD rats' brains, which considered crucial cell organelle to supply for brain.Type 2 diabetes mellitus (T2DM) rat models were established by using high fat diet and streptozotocin (STZ) (30 mg/kg, ip). The evaluation insulin sensitivity...

// Chunyan Gu 1,* , Ye Yang Hong Xiang 2 Shu Li Lina Liang 4 Hua Sui Libin Zhan 1,2 and Xiaoguang Lu 3 1 Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China The Second Affiliated Hospital Dalian University, Dalian, Liaoning, Department Emergency Zhongshan Hospital, Institute Integrative * These authors contributed equally to this work Correspondence to: Zhan, email: Lu, Keywords : type diabetes; zucker diabetic fatty rat; gastrointestinal microbe; 16S rRNA;...

// Chunyan Gu 1,* , Wen Zhou 2,* Wang 1 Hong Xiang 3 Huiying Xu 2 Lina Liang 4 Hua Sui Libin Zhan 2,3 and Xiaoguang Lu 5 School of Medicine Life Science, Nanjing University Chinese Medicine, Nanjing, Jiangsu, China Basic Medical College, The Second Affiliated Hospital Dalian University, Dalian, Liaoning, Institute Integrative Department Emergency Zhongshan Hospital, * These authors have contributed equally to this work Correspondence to: Zhan, email: Lu, Keywords : ZiBuPiYin recipe,...

Background and Purpose: Neurodegenerative diseases are associated with metabolic disturbances. Pyruvate dehydrogenase E1 component subunit alpha (PDHA1) is an essential in the process of glucose metabolism, its deficiency exists various such as Alzheimer's disease (AD), epilepsy, Leigh's syndrome, diabetes-associated cognitive decline. However, exact role PDHA1 neurodegenerative remains to be elucidated. In this study, we explored effect on function molecular mechanism. Methods: A...

Diabetes-associated cognitive decline (DACD), one of the complications type 2 diabetes (T2DM), correlates significantly with disorder in glycolipid metabolism, insulin/leptin resistance, and accumulation β-amyloid (Aβ). Although gut microbiota transplantation (GMT), a novel non-invasive physiotherapy strategy, has been promising intervention to alleviate symptoms T2DM, its protective effect on progressive remains elusive. Here, we transplanted healthy or donor rats into ZDF LZ rats,...

Members of the epidermal growth factor family play important roles in regulation cell growth, proliferation, and survival. However, specific each member with respect to brain injury are not well understood. Gene chip assay screens have revealed drastic increases expression members amphiregulin epiregulin following lipopolysaccharide stimulation, which activates an immune response. Both activity endoplasmic reticulum stress activated during cerebral ischemia. We found that levels were...