A5100677543- Biosensors and Analytical Detection
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Advanced Biosensing Techniques and Applications
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrowetting and Microfluidic Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Immune cells in cancer
- Advanced Combustion Engine Technologies
- Lipid Membrane Structure and Behavior
- Biodiesel Production and Applications
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Reproductive Biology and Fertility
- Advanced Sensor and Energy Harvesting Materials
- Cancer Cells and Metastasis
- Vehicle emissions and performance
- Immunotherapy and Immune Responses
Xi'an University of Architecture and Technology
2025
Shanghai Jiao Tong University
2024-2025
Dalian University of Technology
2013-2024
Dalian Institute of Chemical Physics
2011-2024
Chinese Academy of Sciences
2011-2024
Northwest A&F University
2024
Renji Hospital
2024
Peking University
2024
Suzhou Institute of Biomedical Engineering and Technology
2024
Peking University Third Hospital
2024
Abstract Here we present a simple and low‐cost production method to generate paper‐based microfluidic devices with wax for portable bioassay. The patterning introduced here included three different ways: (i) painting pen, (ii) printing an inkjet printer followed by (iii) directly. whole process was easy operate could be finished within 5–10 min without the use of clean room, UV lamp, organic solvent, etc . Horse radish peroxidase, BSA glucose assays were conducted verify performance...
Paper-based microfluidics is a promising technology to develop simple, low-cost, portable, and disposable diagnostic platform for resource-limited settings. Here we report the fabrication of paper-based microfluidic devices in nitrocellulose membrane by wax printing protein immobilization related applications. The process, which can be finished within 10 min, includes mainly baking steps. Wax patterning will form hydrophobic regions membrane, used direct flow path or separate reaction zones....
Abstract Using an ORF kinome screen in MCF-7 cells treated with the CDK4/6 inhibitor ribociclib plus fulvestrant, we identified FGFR1 as a mechanism of drug resistance. FGFR1-amplified/ER+ breast cancer and transduced were resistant to fulvestrant ± or palbociclib. This resistance was abrogated by treatment FGFR tyrosine kinase (TKI) lucitanib. Addition TKI erdafitinib palbociclib/fulvestrant induced complete responses patient-derived-xenografts. Next generation sequencing circulating tumor...
Despite recent advances in single-cell genomic, transcriptional, and mass-cytometric profiling, it remains a challenge to collect highly multiplexed measurements of secreted proteins from single cells for comprehensive analysis functional states. Herein, we combine spatial spectral encoding with polydimethylsiloxane (PDMS) microchambers codetection 42 immune effector cells, representing the highest multiplexing recorded date secretion assay. Using this platform profile differentiated...
Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more serum than normal and these overexpress a certain number of cancer-related biomarkers. However, capture biomarker analysis for clinical application are technically challenging. In this study, we developed microfluidic chip immunocapture quantification circulating from small sample volume applied device study. Circulating EpCAM-positive were measured 6 cases breast patients 3 healthy...
Abstract Objectives Carcinoma-associated fibroblasts (CAFs) have been known to promote cancer progression by modifying the primary tumor microenvironment. We aimed elucidate intercellular communication between CAFs and secondary organs in salivary adenoid cystic carcinoma (SACC) metastasis. Methods Pre-metastatic metastatic animal models of SACC were established using extracellular vesicles (EVs) from cells. Lung (LFs) treated with EVs their transcriptomic alterations identified RNA...
Secreted proteins dictate a range of cellular functions in human health and disease. Because the high degree heterogeneity and, more importantly, polyfunctionality individual cells, there is an unmet need to simultaneously measure array from single cells rapidly assay large number (more than 1000) parallel. We describe simple bioanalytical platform consisting subnanoliter microchambers integrated with high-density antibody barcode microarrays for highly multiplexed protein detection over...
Comparing isolated cells with cell populations uncovers the contributions of autocrine and paracrine signaling to secretion cytokines by macrophages.
We report on the development of a nanowire substrate-enabled laser scanning imaging cytometry for rare cell analysis in order to achieve quantitative, automated, and functional evaluation circulating tumor cells. Immuno-functionalized arrays have been demonstrated as superior material capture cells from heterogeneous populations. The method enables large-area, automated quantitation captured rapid cellular parameters (e.g., size, shape, signaling protein) at single-cell level. This...
Magnesium (Mg) alloys have revolutionized the application of temporary load-bearing implants as they meet both engineering and medical requirements. However, rapid degradation Mg under physiological conditions remains major obstacle hindering wider use Mg-based implants. Here we developed a simple method preparing nanoscale MgF2 film on Mg-Nd-Zn-Zr (denoted JDBM) alloy, aiming to reduce corrosion rate well improve biological response. The JDBM alloy exposed artificial plasma is reduced by...
Extracellular vesicles (EVs) are important intercellular mediators regulating health and diseases. Conventional methods for EV surface marker profiling, which was based on population measurements, masked the cell-to-cell heterogeneity in quantity phenotypes of secretion. Herein, by using spatially patterned antibody barcodes, we realized multiplexed profiling single-cell secretion from more than 1,000 single cells simultaneously. Applying this platform to profile human oral squamous cell...
Purpose:FGFR1 amplification occurs in approximately 15% of estrogen receptor-positive (ER+) human breast cancers. We investigated mechanisms by which FGFR1 confers antiestrogen resistance to ER+ cancer.Experimental Design: tumors from patients treated with letrozole before surgery were subjected Ki67 IHC, FISH, and RNA sequencing (RNA-seq). ER+/FGFR1-amplified cancer cells, patient-derived xenografts (PDX) siRNA or the FGFR tyrosine kinase inhibitor lucitanib. Endpoints cell/xenograft...
Accurately placing very small amounts of electrolyte on tiny micro-supercapacitors (MSCs) arrays in close proximity is a major challenge. This difficulty hinders the development densely-compact monolithically integrated MSCs (MIMSCs). To overcome this grand challenge, we demonstrate controllable directed assembly strategy for precise isolation densely-packed at micron scale, achieving scalable production MIMSCs with ultrahigh areal number density and output voltage. We fabricate patterned...
A droplet-based microfluidic device integrated with a novel floatage-based trap array and tapered immobilization channel was presented for characterizing the neurotoxin-induced multiple responses in individual Caenorhabditis elegans (C. elegans) continuously. The established enabled evaluations of movement fluorescence imaging analysis C. simultaneously. utility this demonstrated by pharmacological evaluation neurotoxin (6-hydroxydopamine, 6-OHDA) triggered mobility defects, neuron...
The poor metabolic ability of cell lines fails to meet the requirements an in vitro model for drug interaction testing which is crucial development or clinical application drugs. Herein, we describe a liver sinusoid-on-a-chip device composed four kinds transformed (HepG2 cells, LX-2 EAhy926 and U937 cells) that were ordered physiological distribution with artificial blood flow biliary efflux flowing opposite direction. This microfluidic applied three-dimensional culturing HepG2 cells high...
β-cell dysfunction is one of the core pathogenetic mechanisms type 2 diabetes mellitus (T2DM). However, there are currently no effective therapeutic strategies to preserve mass and function. The role islet macrophage phenotype reprogramming in has attracted great attention. Given that advanced glycation end products (AGEs) major pathogenic factors T2DM, we investigated effect AGEs on activation their dysfunction.We examined cytokine secretion, M1 M2 macrophage-associated marker expression...
This paper describes the use of camera cell phone to detect gold nanoparticle-labeled immunoassay results on microfluidic chip. On-chip heterogeneous between anti-human IgG and human (hIgG) was performed were amplified by nanoparticle-enhanced silver staining transform nano-scale probe into detectable precipitation film. The detection obtained showed good correlation with concentration hIgG. scheme is simple, low cost easy has significant promise for portable diagnosis point care testing platform.
Abstract MDR1 is highly expressed in MDR A2780DX5 ovarian cancer cells, SGC7901R gastric cells and recurrent tumours. It pumps cytoplasmic agents out of leading to decreased drug accumulation making susceptible multidrug resistance. Here, we identified that miR‐495 was predicted target ABCB1 , which encodes protein MDR1. To reduce the efflux reverse overexpressed a mimic A2780DX transplanted tumours vivo . The results indicated expression above or suppressed subsequently decreased, cell...