A5101454002- Extracellular vesicles in disease
- Retinal Diseases and Treatments
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Ocular Oncology and Treatments
- Angiogenesis and VEGF in Cancer
- Circular RNAs in diseases
- Phagocytosis and Immune Regulation
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Retinopathy of Prematurity Studies
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- Retinal Development and Disorders
- Immunotherapy and Immune Responses
- Estrogen and related hormone effects
- Retinal Imaging and Analysis
- Epigenetics and DNA Methylation
- Protease and Inhibitor Mechanisms
Centre Hospitalier Universitaire Sainte-Justine
2014-2025
Université de Montréal
2015-2025
Dongguan People’s Hospital
2024
Guangdong Medical College
2024
Chongqing Medical University
2023
Kunming Medical University
2022
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2018-2020
University of Electronic Science and Technology of China
2018-2020
Beijing Obstetrics and Gynecology Hospital
2018
Capital Medical University
2018
Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more serum than normal and these overexpress a certain number of cancer-related biomarkers. However, capture biomarker analysis for clinical application are technically challenging. In this study, we developed microfluidic chip immunocapture quantification circulating from small sample volume applied device study. Circulating EpCAM-positive were measured 6 cases breast patients 3 healthy...
Recent studies suggested an association of endothelial microRNA-126 (miR-126) with type 2 diabetes mellitus (T2DM). In the current study, we examined whether circulating miR-126 is associated T2DM and pre-diabetic syndrome. The study included 82 subjects impaired glucose tolerance (IGT), 75 fasting (IFG), 160 patients newly diagnosed T2DM, 138 healthy individuals. Quantitative polymerase chain reaction (qPCR) was used to examine serum miR-126. Serum significantly lower in IGT/IFG than...
Abstract PM 2.5 is the main particulate air pollutant whose aerodynamic diameter less than micron. The inflammation of various respiratory diseases are associated with inhalation. Pro-inflammatory cytokine IL-1β generated from effected cells usually plays a crucial role in many kinds lung inflammatory reactions. exacerbation Th immune responses identified some related diseases. To elucidate underlying mechanism -induced acute inflammation, we exposed Balb/c mice to intratracheally and...
Melphalan is an efficient chemotherapeutic agent that currently used to treat retinoblastoma (Rb); however, the inherent risk of immunogenicity and hazardous integration this drug in healthy cells inevitable. MicroRNAs are short non-coding single-stranded RNAs affect a vast range biological processes. Previously, we focused on regulatory role miR-181a during cancer development progression. In manuscript, 171 nm switchable lipid nanoparticles (LNP) co-delivered melphalan with encapsulation...
Recent studies have demonstrated that lymphocyte-derived microparticles (LMPs) impair endothelial cell function. However, no data currently exist regarding the contribution of LMPs in regulation angiogenesis. In present study, we investigated effects on angiogenesis vivo and vitro strongly suppressed aortic ring microvessel sprouting corneal neovascularization. vitro, considerably diminished human umbilical vein survival proliferation a concentration-dependent manner. Mechanistically,...
Glioblastoma multiforme (GBM) is the most common and lethal primary brain cancer. Current pharmacological interventions marginally increase 12-month overall survival of patients with GBM. Among novel therapeutic strategies being pursued, micro-RNAs, a class non-coding RNAs, are receiving considerable attention for their regulation several pathways implicated in tumorigenesis survival. Notably, microRNA-181a-5p (miR-181a) has consistently been reported to be downregulated GBM clinical...
The mammillary body (MB), and its axonal projections to the thalamus(mammillothalamic tract, MTT) tegmentum (mammillotegmental tract,MTEG), are components of a circuit involved in spatial learning. bHLH-PAS transcription factors SIM1 SIM2 co-expressed developing MB. We have found that MB neurons generated they survive at least until E18.5 embryos lacking both Sim1 Sim2(Sim1-/-;Sim2-/-). However, MTT MTEG histologically absent Sim1-/-;Sim2-/- embryos, reduced but bearing one or two copies...
Nonstop proliferation and vigorous neovascularization are two prominent characteristics of cancer. Antiangiogenic therapy has emerged as an important modality in treatment solid tumors. Our previous work demonstrated that microparticles derived from apoptotic T-lymphocytes (LMPs) not only reduced the viabilities high-proliferating cells, but also exhibited potent anti-angiogenic effects through inhibition vascular endothelial growth factor (VEGF)/VEGF receptor 2 signalling pathway. In...
Microparticles possess therapeutic potential regarding angiogenesis. We have demonstrated the contribution of apoptotic human CEM T lymphocyte-derived microparticles (LMPs) as inhibitors angiogenic responses in animal models inflammation and tumor growth. In present study, we characterized antivascular endothelial growth factor (VEGF) effects LMPs on pathological angiogenesis an model oxygen-induced retinopathy explored role receptor-mediated endocytosis retinal cells (HRECs). dramatically...
Background. The purpose of the present study was to assess feasibility using miR-126 in urine as a biomarker for diabetic nephropathy. Methods. miRNAs were extracted from samples T2DM patients with nephropathy (DN;<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">92</mml:mn></mml:math>), without DN (<mml:math id="M2"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">86</mml:mn></mml:math>), and 85...
Background: Type 2 diabetes mellitus (T2DM) is strongly correlated with Alzheimer's disease (AD). Rapamycin has important uses in oncology, cardiology and transplantation medicine. This study aims to investigate effects of rapamycin on AD hippocampus T2DM rat by AMPK/mTOR signaling pathway.Methods: Morris water maze test was applied evaluate the learning memory abilities. The fasting plasma glucose (FBG), glycosylated haemoglobin, total cholesterol, triglyceride serum insulin level were...
Lipid nanoparticles (LNPs) have established their position as nonviral vectors for gene therapy. Tremendous efforts been made to modulate the properties of LNPs unleash full clinical potential. Among strategies being pursued, layer-by-layer (LbL) technique has gained considerable attention in biomedical field. Illuminated by our previous work, here we investigate if LbL approach could be used modify LNP cores formulated with three different ionizable lipids: DODMA, MC3, and DODAP....
Haploinsufficency of Sim1, which codes for a basic helix-loop-helix-PAS (PER-ARNT-SIM) transcription factor, causes hyperphagia in mice and humans, without decrease energy expenditure. Sim1 is expressed several areas the brain, including developing postnatal paraventricular nucleus (PVN), region hypothalamus that controls food intake. We have previously found number PVN cells decreased Sim1+/- mice, suggesting their caused by developmental mechanism. However, possibility functions to control...
Although previous studies have demonstrated that hypoxia induces retinal ganglion cell (RGC) apoptosis and transient ischemia upregulates the expression of lysophosphatidic acid (LPA) receptors, it remains to be determined whether LPA(1) receptor mediates RGC degeneration during retinopathy prematurity (ROP). By using an immortalized line (RGC-5), primary neonatal cultures, oxygen-induced (OIR) model ROP, authors explored potential mechanisms thereof.OIR was induced by exposing rat pups...
Abstract Pathological choroidal neovascularization (CNV) is the common cause of vision loss in patients with age-related macular degeneration (AMD). Macrophages possess potential angiogenic function CNV. We have demonstrated that human T lymphocyte-derived microparticles (LMPs) exert a potent antiangiogenic effect several pathological models. In this study, we investigated alteration proangiogenic properties macrophages by LMPs treatment vitro and vivo regulated expression...
Abstract Background It remains unclear etiology of cartilaginous tissues in osteoarthritis (OA) lesions. In this study, we hypothesized the accumulation hypoxia-inducible factor (HIF) and activated apoptosis relate to condylar cartilage degeneration vivo. Methods Malocclusion stress was applied for 2 weeks, 4 weeks 8 induce an OA-like lesion animal model rats. Histological analysis performed by H&E staining Safranin O/fast green staining. The expression levels protein were examined...
Using a community-based population cross-sectional study, we investigated the validity of an enzymatic method for glycated albumin (GA) measurements and evaluated its utility as diagnostic tool diabetes mellitus (DM). In total, 1,211 participants from city Harbin, People9s Republic China, were enrolled in study. A receiver operating characteristic (ROC) analysis GA, fasting plasma glucose (FPG) haemoglobin A<sub>1c</sub> (HbA<sub>1c</sub>) diagnosed undiagnosed DM compared, based on...