A5101596075- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Endometriosis Research and Treatment
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Reproductive System and Pregnancy
- Galectins and Cancer Biology
- MicroRNA in disease regulation
- Helicobacter pylori-related gastroenterology studies
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Microbial metabolism and enzyme function
- Pregnancy and preeclampsia studies
- Estrogen and related hormone effects
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Inflammatory mediators and NSAID effects
- Monoclonal and Polyclonal Antibodies Research
- Hedgehog Signaling Pathway Studies
- Proteoglycans and glycosaminoglycans research
- Connective tissue disorders research
- Tissue Engineering and Regenerative Medicine
- Angiogenesis and VEGF in Cancer
- Salivary Gland Tumors Diagnosis and Treatment
Yunnan Provincial Hospital of Traditional Chinese Medicine
2024
Dalian Medical University
2013-2023
Shanghai Stomatological Hospital
2023
Fudan University
2023
Core Laboratories (United States)
2016
Jinan University
2011-2012
Increasing attention has been attracted by exosomes in blood-based diagnosis because cancer cells release more serum than normal and these overexpress a certain number of cancer-related biomarkers. However, capture biomarker analysis for clinical application are technically challenging. In this study, we developed microfluidic chip immunocapture quantification circulating from small sample volume applied device study. Circulating EpCAM-positive were measured 6 cases breast patients 3 healthy...
Abstract Objectives Carcinoma-associated fibroblasts (CAFs) have been known to promote cancer progression by modifying the primary tumor microenvironment. We aimed elucidate intercellular communication between CAFs and secondary organs in salivary adenoid cystic carcinoma (SACC) metastasis. Methods Pre-metastatic metastatic animal models of SACC were established using extracellular vesicles (EVs) from cells. Lung (LFs) treated with EVs their transcriptomic alterations identified RNA...
Abstract Carcinoma-associated fibroblasts (CAFs) are the main cellular components of tumor microenvironment and promote cancer progression by modifying extracellular matrix (ECM). The tumor-associated ECM is characterized collagen crosslinking catalyzed lysyl oxidase (LOX). Small vesicles (sEVs) mediate cell-cell communication. However, interactions between sEVs remain unclear. Here, we demonstrated that released from oral squamous cell carcinoma (OSCC)-derived CAFs induce crosslinking,...
Successful embryo implantation requires the establishment of a receptive endometrium. Poor endometrial receptivity has generally been considered as major cause infertility. Protein glycosylation is associated with many physiological and pathological processes. The fucosylation catalyzed by specific fucosyltransferases. Fucosyltransferase IV (FUT4) key enzyme for biosynthesis α1,3-fucosylated glycans carried glycoproteins, previous studies showed FUT4 expression changed dynamically during...
// Jing Kong 1 , Yong Luo 2 Dong Jin Fan An Wenyuan Zhang Lilu Liu Jiao Li Shimeng Fang Xiaojie Xuesong Yang 3 Bingcheng Lin 2, 4 Tingjiao College of Stomatology, Dalian Medical University, Dalian, China Faculty Chemical, Environmental and Biological Science Technology, Technology Department Biochemistry Molecular Biology, Liaoning Provincial Core Lab Glycobiology Glycoengineering, Biotechnology, Institute Chemical Physics, Chinese Academy Sciences, Correspondence to: Liu, email:...
The epithelial‐to‐mesenchymal transition (EMT), a process involving the breakdown of cell–cell junctions and loss epithelial polarity, is closely related to cancer metastasis invasion. cell adhesion molecule (EpCAM) type I transmembrane protein expressed in majority normal tissues overexpressed human cancers including breast cancer. EpCAM plays an important role progression. We showed that participated TGF‐β1‐induced EMT. TGF‐β1 treatment MCF‐7 cells was shown induce expression, which...
Epithelial cell adhesion molecule (EpCAM) is a type I transmembrane protein that expressed in the majority of normal epithelial tissues and overexpressed most cancers including breast cancer, where it plays an important role cancer progression. However, mechanism by which EpCAM promotes progression not understood. In this study, we found expression was increased tumor tissue from patients compared to healthy patients. Overexpression cells enhanced growth vitro invasiveness, whereas small...
Carcinoma-associated fibroblasts (CAFs) are critical in determining tumor invasion and metastasis. However the role of CAFs salivary gland adenoid cystic carcinoma (ACC) is poorly understood. In this study, we isolated primary from two ACC patients. ACC-derived expressed typical CAF biomarkers showed increased migration activity. Conditioned medium collected significantly promoted cell invasion. Co-culture with cells a microfluidic device further revealed that localized at front followed...
Epithelial cell adhesion molecule (EpCAM), which is a transmembrane glycoprotein, related to tumor progression. We demonstrated that EpCAM plays important roles in proliferation, apoptosis, and metastasis during breast cancer (BC) But the role of N-glycosylation aggressiveness not clear. Here, we evaluated stemness epithelial-mesenchymal transition (EMT) characteristics. overexpression increases expression markers (NANOG，SOX2, OCT4) EMT (N-cadherin vimentin) under condition hypoxia BC....
Helicobacter pylori vacA and cagA genes have significant genetic heterogenicity, resulting in different clinical outcomes. Northeast part of China has reported high prevalence H. infections gastric cancer. Hence, we investigated the genotypes with outcomes China. Gastric tissue samples (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>169</mml:mn></mml:math>), chronic gastritis (GIs), ulcer (GU), cancer (GC) were analysed for 16S...
Resistance to fluoropyrimidine-based chemotherapy is the main reason for failure of cancer treatment, and drug resistance associated with an inability tumor cells undergo apoptosis in response treatment. Alterations expression epithelial cell adhesion molecule (EpCAM) affect sensitivity or anticancer treatment activity intracellular signaling pathways. However, role EpCAM induction breast remains unclear. Here, we investigated effect gene knockdown on chemosensitivity 5-fluorouracil (5-FU)...
Extracellular vesicles (EVs) from cancer cells remodel distant organs to promote metastasis in vivo. A biomimetic microsystem may compensate costly and time‐consuming animal models accelerate the study of EV organotropism. tissue‐based liver–kidney‐on‐a‐chip is developed with precision‐cut tissue slices (PTSs) cultured represent individual organs. The organotropism breast EVs modeled using microsystem. traditional model used investigate physiological similarity microfluidic models. It...
Mimicking<italic>in vivo</italic>angiogenesis on a microfluidic model and application testing drug potential to inhibit angiogenesis.
Epithelial cell adhesion molecule (EpCAM) expression is elevated in breast cancer tissue, and correlates with the metastasis adhesion. Although EpCAM glycosylation supposed to be associated its function, contribution of N-glycosylation function remains unclear. Here we analyzed ability cells. The results showed that was mutation decreased capacity. correlated lower levels integrin β1 fibronectin. We also found effect on regulated via FAK/Akt/Gsk-3β/β-catenin signaling pathway, which further...
Glycosylation of cell surface proteins plays an important role in the regulation apoptosis. It has been demonstrated that knockdown epithelial adhesion molecule promoted apoptosis, inhibited proliferation, and caused cell-cycle arrest. In this study, we investigated whether how N-glycosylation influenced apoptosis breast cancer cells. We applied mutation plasmid to express deglycosylation then study its function. Our results showed proliferation. Deglycosylation enhanced cytotoxic effect...
Summary Nonsense mutations that result in expression of truncated, N-terminal fragments the APC tumour suppressor protein are found most sporadic and some hereditary colorectal cancers. These can cause tumourigenesis by eliminating β-catenin binding sites from APC, which leads to upregulation induction oncogenes such as MYC. Here we show three distinct experimental model systems, an fragment (N-APC) results loss directionality, but not speed, cell motility independently changes regulation....
ABSTRACT Lewis Y (LeY) is a carbohydrate tumor‐associated antigen. The majority of cancer cells derived from epithelial tissues express LeY type difucosylated oligosaccharides. Fucosyltransferase IV (FUT4) an essential enzyme that catalyzes the synthesis In previous study we reported FUT4 associated with cell proliferation; however, despite important role in proliferation and apoptosis, little known about mechanisms underlying regulation transcription. current investigated transcription...
At present, biopsy specimens, cancer cell lines and tissues obtained by gastric surgery are used in the study analysis of cancer, including molecular mechanisms proteomics. However, fibroblasts other tissue components may interfere with these techniques. Therefore, present aimed to develop a procedure for isolation viable human epithelial cells from surgical tissues. A method was developed culture using fresh, surgically excised evaluated immunocytochemistry, periodic acid‑Schiff (PAS)...
Abstract The transmembrane glycoprotein epithelial cell adhesion molecule (EpCAM) is overexpressed in most cancers including breast cancer, where it plays an important role cancer progression. Previous study has demonstrated that knockdown of EpCAM inhibits growth and metastasis via inhibition the Ras/Raf/ERK signaling pathway matrix metallopeptidase‐9 (MMP‐9). Although glycosylation believed to be associated with function EpCAM, contribution N‐glycosylation this remains unclear. We...