A5100715400- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- RNA modifications and cancer
- Reproductive System and Pregnancy
- Endoplasmic Reticulum Stress and Disease
- Circular RNAs in diseases
- Pregnancy and preeclampsia studies
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Tryptophan and brain disorders
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- DNA and Nucleic Acid Chemistry
- Plant Virus Research Studies
- Asthma and respiratory diseases
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
Zhengzhou University
2025
Shandong University
2012-2024
Dalian Medical University
2012-2024
Shandong Institute for Product Quality Inspection
2010-2024
Tianjin Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2024
Hebei Medical University
2017-2024
Nanning Normal University
2024
Inner Mongolia People's Hospital
2024
National Heart Lung and Blood Institute
2018-2023
Abstract The cotton bollworm Helicoverpa armigera , is one of the world’s major pest agriculture, feeding on over 300 hosts in 68 plant families. Resistance cases to most insecticide classes have been reported for this insect. Management agroecosystems relies a better understanding how it copes with phytochemical or synthetic toxins. We used genome editing knock out cluster nine P450 genes and show that significantly reduces survival rate insect when exposed two host chemicals insecticides....
Mammalian genomes have multiple enhancers spanning an ultralong distance (>megabases) to modulate important genes, but it is unclear how these coordinate achieve this task. We combine multiplexed CRISPRi screening with machine learning define quantitative enhancer-enhancer interactions. find that the enhancer network has a nested multilayer architecture confers functional robustness of gene expression. Experimental characterization reveals epistasis maintained by three-dimensional...
Expansion of polyglutamine (polyQ) tract may cause protein misfolding and aggregation that lead to cytotoxicity neurodegeneration, but the underlying mechanism remains be elucidated. We applied ataxin-3 (Atx3), a polyQ tract-containing protein, as model study sequestration normal cellular proteins. found aggregates formed by polyQ-expanded Atx3 sequester its interacting partners, such P97/VCP ubiquitin conjugates, into inclusions through specific interactions both in vitro cells. Moreover,...
Alternaria blotch disease of apple (Malus×domestica Borkh.), caused by the pathotype alternata, is one most serious fungal diseases to affect apples. To develop an understanding how apples respond A. alternata (AAAP) infection, we examined host transcript accumulation over period between 0 hours and 72 post AAAP inoculation. Large-scale gene expression analysis was conducted compatible interactions 'Starking Delicious' cultivar using RNA-Seq digital (DGE) profiling methods. Our results show...
Impaired function of the endoplasmic reticulum (ER stress) is a hallmark many human diseases including stroke. To restore ER in stressed cells, unfolded protein response (UPR) induced, which activates 3 stress sensor proteins activating transcription factor 6 (ATF6). ATF6 then cleaved by proteases to form short-form (sATF6), factor. determine extent activation UPR branch defines fate and neurons after stroke, we generated conditional tamoxifen-inducible sATF6 knock-in mouse. express...
Asthma, allergic rhinitis, and pollinosis are prevalent respiratory conditions that often co-occur, suggesting common genetic environmental causes. While significant progress has been made in identifying loci European populations, the architecture East Asian populations remains poorly understood. Using GWAS summary statistics from BioBank Japan, we performed multi-trait genome-wide association studies (MTAG) to quantify overlap among asthma, Asians. Genetic correlation analysis revealed...
Impaired protein homeostasis induced by endoplasmic reticulum dysfunction is a key feature of variety age-related brain diseases including stroke. To restore function impaired stress, the unfolded response activated. A prosurvival pathway controlled stress sensor (inositol-requiring enzyme-1), XBP1 (downstream X-box-binding protein-1), and O-GlcNAc (O-linked β-N-acetylglucosamine) modification proteins (O-GlcNAcylation). Stroke impairs function, which activates response. The rationale this...
Abstract The three-dimensional genomic structure plays a critical role in gene expression, cellular differentiation, and pathological conditions. It is pivotal to elucidate fine-scale chromatin architectures, especially interactions of regulatory elements, understand the temporospatial regulation expression. In this study, we report Hi-TrAC as proximity ligation-free, robust, sensitive technique profile genome-wide at high-resolution among elements. detects looping accessible regions single...
Methamphetamine (METH) abuse causes serious health problems worldwide, and long-term use of METH disrupts the blood-brain barrier (BBB). Herein, we explored potential mechanism endoplasmic reticulum (ER) stress in METH-induced BBB endothelial cell damage vitro therapeutic inhibitors for disruption C57BL/6J mice. Exposure immortalized BMVEC (bEnd.3) cells to significantly decreased viability, induced apoptosis, diminished tightness monolayers. activated ER sensor proteins, including PERK,...
Hypoxia‐induced neuroinflammation typically causes neurological damage and can occur during stroke, neonatal hypoxic‐ischemic encephalopathy, other diseases. Propofol is widely used as an intravenous anesthetic. Studies have shown that propofol has antineuroinflammatory effect. However, the underlying mechanism remains to be fully elucidated. Thus, we aimed investigate beneficial effects of against hypoxia‐induced elucidated its potential cellular biochemical mechanisms action. In this...
Development of leaf margins is an important process in morphogenesis. CIN-clade TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF) transcription factors are known to have redundant roles specifying margins, but the specific mechanisms through which individual genes function remain elusive. In this study, we report that CIN-TCP gene TCP5 involved repressing initiation and outgrowth serrations by activating two key regulators margin development, Class II KNOX factor KNAT3 BEL-like SAW1. Specifically,...
UCHs [Ub (ubiquitin) C-terminal hydrolases] are a family of deubiquitinating enzymes that often thought to only remove small peptide tails from Ub adducts. Among the four identified date, neither UCH-L3 nor UCH-L1 can catalyse hydrolysis isopeptide chains, but UCH-L5 when it is present in PA700 complex proteasome. In paper, we report UCH domain UCH-L5, different and UCH-L3, by itself process K48-diUb (Lys48-linked di-ubiquitin) substrate cleaving bond between two units. The catalytic...
To evaluate the effect of age on response brains to an ischemic challenge, we subjected young and aged mice transient forebrain ischemia, analyzed heat shock unfolded protein response, ubiquitin conjugation SUMO conjugation, O-linked β-N-acetylglucosamine modification proteins (O-GlcNAcylation). The most prominent age-related difference was inability activate O-GlcNAcylation. Considering many reports protective role O-GlcNAcylation in various stress conditions including myocardial this...
Burn injuries often leave behind a "stasis zone", region of tissue critically important for determining both the severity injury and potential recovery. To understand intricate cellular epigenetic changes occurring within this critical zone, we utilized single-cell assay transposase-accessible chromatin sequencing (scATAC-seq) to profile over 31,500 cells from healthy rat skin stasis zone at nine different time points after burn injury. This comprehensive approach revealed 26 distinct cell...
Human ataxin 7 (Atx7) is a component of the deubiquitination module (DUBm) in Spt-Ada-Gcn5-acetyltransferase (SAGA) complex for transcriptional regulation, and expansion its polyglutamine (polyQ) tract leads to spinocerebellar ataxia type 7. However, how polyQ Atx7 affects DUBm function remains elusive. We investigated effects polyQ-expanded on ubiquitin-specific protease (USP22), an interacting partner functioning histone H2B. The results showed that inclusions or aggregates formed by...
Small natural molecules derived from medicinal plants have long been considered as a rich pool of novel therapeutic medicine. Carnosol is bioactive diterpene compound Rosmarinus officinalis (Rosemary) and Salvia officinalis, herbs widely used in traditional medicine for the treatment various inflammatory diseases. In this study, we investigated effects mechanism action carnosol experimental autoimmune encephalomyelitis (EAE), an animal model multiple sclerosis (MS). effectively ameliorated...