A5100335709- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Nicotinic Acetylcholine Receptors Study
- Metabolomics and Mass Spectrometry Studies
- Enzyme Structure and Function
- Ion channel regulation and function
- Protein Hydrolysis and Bioactive Peptides
- Neuroscience and Neuropharmacology Research
- Chemical Synthesis and Analysis
- Gut microbiota and health
- Advanced biosensing and bioanalysis techniques
- Gene Regulatory Network Analysis
- Phytochemistry and Biological Activities
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Carbohydrate Chemistry and Synthesis
- Biochemical and Structural Characterization
- Polyamine Metabolism and Applications
- Plant Reproductive Biology
- Advanced Glycation End Products research
- Biofuel production and bioconversion
- Cancer-related gene regulation
- Bone Metabolism and Diseases
- Biosensors and Analytical Detection
- Insect Utilization and Effects
Xiamen University
2015-2025
Yunnan University
2022-2025
Michigan State University
2025
Nanning Normal University
2021-2024
Jiujiang University
2024
Tongji Hospital
2020-2023
Huazhong University of Science and Technology
2020-2023
Shanghai Ocean University
2022-2023
University of Louisville
2023
Beijing Technology and Business University
2023
We invented a new method for highly efficient and specific enrichment of glycopeptides using two different nanomaterials synergistically. One is boronic-acid-functionalized Fe3O4 nanoparticles, enriching through formation cyclic boronate esters between the boronic acid groups cis–diol on glycopeptides. The other nanomaterial conventional poly(methyl methacrylate) nanobeads, which have strong adsorption toward nonglycopeptides. By optimizing proportion these materials, extremely high...
Two novel collagen peptides derived from Yak bones, UU1 (GASGPMGPR) and K7 (GLPGPM) were demonstrated to be antioxidative in vitro Caenorhabditis elegans. In vitro, shown decent scavenging activities on free radicals of DPPH, ABTS, ·OH ·O–2. Their effects worms, including the lifespans, locomotory behavior, reproduction further evaluated. Whether under normal or thermal conditions, extended survival time accordingly, promoted reproduction. Additionally, remarkably attenuated accumulations...
Background: Osteoporosis has become an inevitable health issue with global aging, and the current drug treatments often have adverse side effects, highlighting need for safer more effective therapies. Collagen-derived peptides are promising alternatives due to their favorable safety profile biological activity. This study aimed investigate osteogenic anti-apoptotic properties of collagen peptide UU1 (GASGPMGPR) in addition its antioxidant Methods: The effects were evaluated MC3T3-E1 cells by...
Abstract This study reports the application of peptide linker in construction bi‐functional formate dehydrogenase (FDH) and leucine (LeuDH) enzymatic complex for efficient cofactor regeneration L‐tert (L‐tle) biotransformation. Seven FDH‐LeuDH fusion enzymes with different were successfully developed displayed both parental enzyme activities. The incorporation order FDH LeuDH was investigated by predicting three‐dimensional structures LeuDH‐FDH models using I‐TASSER server. characterization...
Clostridium butyricum, a new probiotic in recent years, can produce butyric acid and short-chain fatty acids. It has the characteristics of strong alkali resistance, high temperature resistance to most antibiotics, more advantages than other probiotics. However, action mechanism C. butyricum on Eriocheir sinensis is still unclear needs further study. In this study, when was added basic diet, number living bacteria 0, 1 × 106 108 CFU/g, respectively. The E. were randomly divided into three...
An ultrasensitive electrochemical platform for p53 gene via MB-mediated CSD and TdT-mediated signal amplification strategy with detection limit of 0.018 fM.
Inosine 5'-monophosphate dehydrogenase (IMPDH) is one of the crucial enzymes in de novo biosynthesis guanosine nucleotides. It has served as an attractive target immunosuppressive, anticancer, antiviral, and antiparasitic therapeutic strategies. In this study, pharmacophore mapping molecular docking approaches were employed to discover novel Homo sapiens IMPDH (hIMPDH) inhibitors. The Güner-Henry (GH) scoring method was used evaluate quality generated hypotheses. One hypotheses found possess...
How neuromodulatory transmitters diffuse into the extracellular space remains an unsolved fundamental biological question, despite wide acceptance of volume transmission model. Here, we report development a method combining genetically encoded fluorescent sensors with high-resolution imaging and analysis algorithms which permits first direct visualization transmitter diffusion at various neuronal non-neuronal cells. Our reveals that acetylcholine monoamines individual release sites spread...
A novel method based on the conjunction of aldehydes from oxidized glycopeptides to aniline groups magnetic nanoparticles via nonreductive amination is reported for highly selective enrichment N-glycopeptides. For first time, a reaction has been introduced into N-glycoproteome extraction, and correspondingly new type aniline-functionalized nanoparticle designed synthesized.
Background: The induction of haemeoxygenase-1 (HO-1) exerts beneficial effects in the setting endothelial dysfunction obesity. High free fatty acid (FFA) levels are a common feature obesity and primary cause dysfunction. objective our study was to explore HO-1 on FFA-induced rats. Methods: Rats received FFA treatment with either cobalt protoporphyrin (CoPP) induce or stannous (SnPP) inhibit HO-1. Endothelial function determined by measuring endothelium-dependent vasodilatation (EDV). Nitric...
Voltage-gated sodium (NaV) channels are important targets of general anesthetics, including the intravenous anesthetic propofol. Electrophysiology studies on prokaryotic NaV channel NaChBac have demonstrated that propofol promotes activation and accelerates activation-coupled inactivation, but molecular mechanisms these effects unclear. Here, guided by computational docking dynamics simulations, we predict several propofol-binding sites in NaChBac. We then strategically place small...
Diabetes mellitus is characterized by hyperglycemia and excessive production of intermediary metabolites including methylglyoxal (MGO), a reactive carbonyl species that can lead to cell injuries. Interacting with proteins, lipids, DNA, MGO cause dysfunction various tissues, especially the vascular walls where diabetic complications often take place. However, potential targets remain be fully understood. Here we show Kir6.1/SUR2B isoform ATP-sensitive K(+) (K(ATP)) channels likely disrupted...