A5101807918- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
- Genetic and Kidney Cyst Diseases
- Enzyme Structure and Function
- Cancer-related molecular mechanisms research
- Multiple Myeloma Research and Treatments
- Photosynthetic Processes and Mechanisms
- Protein Degradation and Inhibitors
- Fibroblast Growth Factor Research
- Protein Kinase Regulation and GTPase Signaling
- Medicinal Plants and Bioactive Compounds
- Chronic Myeloid Leukemia Treatments
- Metabolism, Diabetes, and Cancer
- Wnt/β-catenin signaling in development and cancer
- Telomeres, Telomerase, and Senescence
- Polyamine Metabolism and Applications
- Glutathione Transferases and Polymorphisms
- Gene Regulatory Network Analysis
- Protist diversity and phylogeny
- Pharmacological Effects of Natural Compounds
- Phytase and its Applications
National Yang Ming Chiao Tung University
2005-2021
National Taiwan University
2014
Taipei City Hospital
2006-2010
National Yang Ming University Hospital
2006
University of California, Riverside
1994-1996
University of California, Los Angeles
1996
We have identified the major enzymatic activity responsible for S-adenosyl-L-methionine-dependent methylation of arginine residues (EC 2.1.1.23) in proteins yeast Saccharomyces cerevisiae. The RMT1 (protein-arginine methyltransferase), formerly ODP1, gene product encodes a 348-residue polypeptide 39.8 kDa that catalyzes both NG-mono- and NG,NG-asymmetric dimethylation variety endogenous polypeptides. A strain which chromosomal was disrupted is viable, but level NG,NG-[3H]dimethylarginine...
Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success producing larger or higher species. Here, we report a linker based sperm-mediated transfer (LB-SMGT) that greatly improves the production efficiency large animals. protein, monoclonal antibody (mAb C), reactive to surface antigen on sperm all tested species including pig,...
Human Aurora kinases have three gene family members: Aurora-A, Aurora-B, and Aurora-C. It is not yet established what the specificity of these are signals relayed by their reactions. Therefore, we employed small pool expression screening to search for downstream substrates Aurora-A. Interestingly, all identified Aurora-A were resistant serve as Aurora-B or Aurora-C, suggesting that members may distinct substrate propagation diverse signaling pathways, even though they share a conserved...
Abstract Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is an RNA/DNA-binding protein involved in chromatin remodeling, RNA processing and the DNA damage response. In addition, increased hnRNPK expression has been associated with tumor development progression. A variety of post-translational modifications have identified shown to regulate function, including phosphorylation, ubiquitination, sumoylation methylation. However, functional significance arginine methylation remains unclear....
The evolutionarily conserved Aurora family kinases, a of mitotic serine/threonine has three members in humans (Aurora-A, -B and -C). Overexpression members, particularly Aurora-A, been reported many human cancers cell lines. In this study, we present evidence based on comparative gene expression analysis via quantitative RT-PCR to delineate the relative contributions these kinases 60 lines statistical five different cancer microarray datasets. demonstrated selective upregulation various...
The phosphatase cell division cycle 25B (Cdc25B) regulates progression. Increased Cdc25B levels are often detected in cancer lines and human cancers have been implicated contributing to tumor growth, potentially by providing cells with the ability bypass checkpoint controls. However, specific mechanism which increased impacts progression is not clear. Here we analyzed Cancer Genome Atlas (TCGA) database found that patients high CDC25B expression had expected poor survival. also a...
The increasing use of high-throughput and large-scale bioinformatics-based studies has generated a massive amount data stored in number different databases. major need now is to explore this disparate find biologically relevant interactions pathways. Thus, the post-genomic era, there clearly for development algorithms that can accurately predict novel protein-protein interaction networks silico. evolutionarily conserved Aurora family kinases have been chosen as model method identify...
PG2 is a botanical drug that mostly composed of Astragalus polysaccharides (APS). Its role in hematopoiesis and relieving cancer-related fatigue has recently been clinically investigated cancer patients. However, systematic analyses its functions are still limited. The aim this study was to use microarray-based expression profiling evaluate the quality consistency from three different product batches biological mechanisms PG2. An integrative molecular analysis approach designed examine...
Objective. This study was designed to clarify the internalization of anti‐DNA antibodies (anti‐DNA) into living cells in pathogenesis systemic lupus erythematosus (SLE) using monoclonal (mAbs).
The activation of p38 mitogen-activated protein kinases (MAPKs) through a phosphorylation cascade is the canonical mode regulation. Here, we report novel mechanism for p38α. We show that Arg49 and Arg149 p38α are methylated by arginine methyltransferase 1 (PRMT1). non-methylation mutations Lys49/Lys149 abolish promotive effect on erythroid differentiation. MAPK kinase 3 (MKK3) identified as major upstream MKK3-mediated R49/149K mutant greatly reduced. This due to profound reduction in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffects of Autophosphorylation on Casein Kinase II Activity: Evidence from Mutations in the .beta. SubunitWey-Jinq Lin, Gwo-Tarng Sheu, and Jolinda A. TraughCite this: Biochemistry 1994, 33, 22, 6998–7004Publication Date (Print):June 7, 1994Publication History Published online1 May 2002Published inissue 7 June 1994https://pubs.acs.org/doi/10.1021/bi00188a032https://doi.org/10.1021/bi00188a032research-articleACS PublicationsRequest reuse...
TIS21 is induced transiently by PMA and a number of extracellular stimuli. Yeast two-hybrid screening has identified three interacting clones from rat cDNA library [Lin, Gary, Yang, Clarke Herschman (1996) J. Biol. Chem 271, 15034–15044]. The amino acid sequence deduced clone 5A shows 96.9% identity with the murine PICK1, protein kinase Cα (PKCα)-binding postulated to act as an intracellular receptor for PKC. A fusion glutathione S-transferase rPICK1 associates translated in vitro,...
Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is an RNA/DNA binding protein involved in diverse cell processes; it also a p53 coregulator that initiates apoptosis under DNA damage conditions. However, the upregulation of hnRNPK correlated with cancer transformation, progression, and migration, whereas regulatory role malignancy remains unclear. We previously showed arginine methylation attenuated U2OS osteosarcoma cells conditions, replacement endogenous methylation-defective mutant...
TIS21 is induced transiently by PMA and a number of extracellular stimuli. Yeast two-hybrid screening has identified three interacting clones from rat cDNA library [Lin, Gary, Yang, Clarke Herschman (1996) J. Biol. Chem 271, 15034–15044]. The amino acid sequence deduced clone 5A shows 96.9% identity with the murine PICK1, protein kinase Cα (PKCα)-binding postulated to act as an intracellular receptor for PKC. A fusion glutathione S-transferase rPICK1 associates translated in vitro,...
Abstract Src, a nonreceptor tyrosine kinase, was the first oncogene identified from an oncogenic virus. Mechanistic studies of Src‐induced transformations aid in understanding pathologic processes underlying tumorigenesis and may provide new strategies for cancer therapy. Although several pathways protein modifications are reportedly involved transformation, detailed mechanisms their regulation remain unclear. Protein methylation is important PTM that widely cellular physiology. In this...
Protein arginine methyltransferase 1 (PRMT1) stimulates erythroid differentiation, but the signaling events upstream are yet to be identified. Ca 2+ plays crucial roles during differentiation. Here, we show that enhances methylation induced differentiation and directly upregulates catalytic activity of recombinant PRMT1 by increasing V max toward substrate heterogeneous nuclear ribonucleoprotein A2. We demonstrate is essential responsible for effect on Depletion suppresses PRMT1‐mediated...
Abstract Protein arginine methylation, catalyzed by protein methyltransferases (PRMTs), plays crucial roles in a variety of cellular processes. Mammalian PRMT1 exists large complex cells, which has been implied modulating the regulatory and catalytic properties this enzyme. Establishment mammalian comparative approach will help to identify putative substrates an authentic condition. Here, we showed that ectopically expressed HEK293 cells not only exhibited comparable endogenous enzyme but...
Chronic myeloid leukemia (CML) is a hematopoietic malignancy characterized by the presence of BCR-ABL oncogene. Therapeutic regimens with tyrosine kinase inhibitors (TKIs) specifically targeting have greatly improved overall survival CML. However, drug intolerance and related toxicity remain. Combined therapy effective in reducing magnitude while increasing therapeutic efficacy and, thus, lowers undesired adverse side effects. The p38 MAPK activity critically linked to pathogenesis number...
Mesenchymal stem cells (MSCs) can differentiate toward various lineages, including the osteogenic lineage, and thus hold great potential for clinic purposes. By using pharmacological inhibitors, protein kinase C (PKC) signaling has been shown to either negatively or positively regulate differentiation of bone, however, due low transfection efficiency in MSCs, role individual PKC isoforms is still not fully understood. In this study, we established a TAT peptide-mediated transduction system...
Casein kinase II (protein CKII) is a heterotetramer consisting of two α and β subunits (1,2). The subunit can be expressed as different gene products which are present and/or α′, depending on the tissue species origin. single product in higher eukaryotes. A comparison sequences from number shows highly conserved (1,3). stable structure dissociable only under denaturing conditions. Thus, complete physical, chemical functional analysis enzyme at molecular level dependent obtaining capable...