A5100704016- PI3K/AKT/mTOR signaling in cancer
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Acute Lymphoblastic Leukemia research
- Multiple Myeloma Research and Treatments
- Protein Degradation and Inhibitors
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- TGF-β signaling in diseases
- Hippo pathway signaling and YAP/TAZ
- Cellular transport and secretion
- Data Mining Algorithms and Applications
- Wnt/β-catenin signaling in development and cancer
- Pregnancy and preeclampsia studies
- Kruppel-like factors research
- Caveolin-1 and cellular processes
- Protein Kinase Regulation and GTPase Signaling
- Signaling Pathways in Disease
- Seaweed-derived Bioactive Compounds
- Single-cell and spatial transcriptomics
- Dialysis and Renal Disease Management
- Transition Metal Oxide Nanomaterials
- Receptor Mechanisms and Signaling
Chinese Academy of Medical Sciences & Peking Union Medical College
2012-2025
Institute of Hematology & Blood Diseases Hospital
2013-2025
Anhui University of Traditional Chinese Medicine
2024
Northwestern Polytechnical University
2023
Ruijin Hospital
2022
Shanghai Jiao Tong University
2022
Kunming Institute of Botany
2021
Chinese Academy of Sciences
2021
National Health Research Institutes
2021
University of Chinese Academy of Sciences
2021
Global analysis of translation regulation has recently been enabled by the development Ribosome Profiling, or Ribo-seq, technology. This approach provides maps ribosome activity for each expressed gene in a given biological sample. Measurements efficiency are generated when Ribo-seq data is analyzed combination with matched RNA-seq expression profiles. Existing computational methods identifying genes differential across samples based on sound principles, but require users to choose between...
Mural cells (MCs) are essential for blood vessel stability and function; however, the mechanisms that regulate MC development remain incompletely understood, in particular those involved specification. Here, we investigated first steps of formation zebrafish using transgenic reporters. Using pdgfrb abcc9 reporters, show onset expression abcc9, a pericyte marker adult mice zebrafish, occurs almost coincidentally with an increment peri-arterial mesenchymal cells, suggesting these...
The development of early B cells, which are generated from hematopoietic stem cells (HSCs) in a series well-characterized stages bone marrow (BM), represents paradigm for terminal differentiation processes. Akt is primarily regulated by phosphorylation at Thr308 PDK1 and Ser473 mTORC2, signaling plays key role hematopoiesis. However, the mTORC2 remains poorly understood. In this study, we investigated functional specifically deleting an integral component, Rictor, system. We demonstrated...
The constitutive hyper-activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target rapamycin (mTOR) signaling pathways has frequently been associated with acute myeloid leukemia (AML). While many inhibitors targeting these have developed, the anti-leukemic effect was not as robust expected. As part molecular link between PI3K/Akt and mTOR kinase, role Rheb1 in AML remains unexplored. Our study aims to explore estimate whether could be a potential treatment.The...
Rationale: Disturbed flow occurring in arterial branches and curvatures induces vascular endothelial cell (EC) dysfunction atherosclerosis. We postulated that disturbed plays important role modulating phosphoprotein expression profiles to regulate functions atherogenesis. Objective: The goal of this study is discover novel site-specific phosphorylation alterations induced by ECs contribute Methods Results: Quantitative phosphoproteomics analysis exposed with low oscillatory shear stress...
TIS21 is induced transiently by PMA and a number of extracellular stimuli. Yeast two-hybrid screening has identified three interacting clones from rat cDNA library [Lin, Gary, Yang, Clarke Herschman (1996) J. Biol. Chem 271, 15034–15044]. The amino acid sequence deduced clone 5A shows 96.9% identity with the murine PICK1, protein kinase Cα (PKCα)-binding postulated to act as an intracellular receptor for PKC. A fusion glutathione S-transferase rPICK1 associates translated in vitro,...
Abstract 3-Phosphoinositide-dependent protein kinase 1 (PDK1) is a pivotal regulator in the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway that have been shown to play key roles functional development of B and T cells via activation AGC kinases during hematopoiesis. However, role PDK1 HSCs has not fully defined. Here we specifically deleted gene hematopoietic system found -deficient exhibited impaired function defective lineage commitment abilities. Lack caused be less quiescent...
PDK1 (phosphoinositide dependent kinase-1) plays an important regulatory role in B cells, T cells and platelets. Less is known about how acts hematopoietic stem (HSCs), especially the fetal liver (FL) during embryonic hematopoiesis, as FL primary organ main site of HSC expansion differentiation. Here, we deleted gene by crossing Vav-Cre transgenic mice with PDK1f/f mice. Using a transplantation assay, found that HSCs from E15.5 Vav-Cre;PDK1f/f embryos are severely impaired compared when or...
A-kinase anchoring proteins (AKAPs) localize cyclic AMP-dependent protein kinase (PKA) to specific regions in the cell and place PKA proximity its phosphorylation targets. A computational model was created identify AKAPs that bind docking/dimerization domain of RII alpha isoform regulatory subunit PKA. The used search entire human proteome, top candidates were tested for an interaction using peptide array experiments. Verified interactions include sphingosine interacting retinoic...
dHG-5 (Mw 5.3 kD) is a depolymerized glycosaminoglycan from sea cucumber
To explore the role of PDK1 in T-ALL development through establishing Notch1-induced mouse model by using Mx1-cre; LoxP system to knock-out PDK1.Cell cycle and apoptosis leukemic cells were detected flow cytometry, relative expression tumor-related genes transcription factors determined quantitative real-time PCR.Notch1-induced with inducible was established successfully. Compared control model, mice showed a significant longer survival time (P<0.01). There no difference cell between mice,...
Myeloid cells have been identified as hematopoietic stem cell (HSC)-regulating cells. However, the mechanisms by which myeloid regulate function of HSCs are not fully defined. Our previous study indicated that over-expanded in Vav1-Cre;Rheb1 f l/fl mice. Here, using vivo and vitro models, we found Rheb1 -deficient neutrophils remodeled bone marrow environment induced expansion . Further studies showed loss impaired neutrophils’ ability to secrete IL-6, led mesenchymal (MSCs) produce more...
High frequent network request pattern in office automation system (OAS) can be described as high itemset when association rule discovery technology is used to mine patterns with log file raw data. To concerned by user (or manager), attention set describe the focus of during mining request. In this paper, extension method discussed for and based on co-occurrence item pair (network behavior pair) presented. Experimental results show that performance our proposed better.