A5038598323- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Kidney Stones and Urolithiasis Treatments
- Acupuncture Treatment Research Studies
- Lung Cancer Diagnosis and Treatment
- Innovations in Medical Education
- Ferroptosis and cancer prognosis
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Global Health Workforce Issues
- Traditional Chinese Medicine Studies
- Healthcare and Venom Research
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- Pelvic floor disorders treatments
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Esophageal Cancer Research and Treatment
- Pediatric Urology and Nephrology Studies
Central South University
2018-2024
Zhongshan Hospital
2015-2024
Fudan University
2015-2024
Sun Yat-sen University
2015-2024
The First Affiliated Hospital, Sun Yat-sen University
2015-2024
Xiangya Hospital Central South University
2018-2024
Jiangnan University
2024
Hangzhou Normal University
2024
Changhai Hospital
2021-2024
Second Military Medical University
2021-2024
Hypoxia induces a group of physiologically important genes such as erythropoietin and vascular endothelial growth factor. These are transcriptionally up-regulated by hypoxia-inducible factor 1 (HIF-1), global regulator that belongs to the basic helix-loop-helix PAS family. Although HIF-1 is heterodimer composed α β subunits, its activity primarily determined hypoxia-induced stabilization HIF-1α, which otherwise rapidly degraded in oxygenated cells. We report identification an...
Fast and strong bio-adhesives are in high demand for many biomedical applications, including closing wounds surgeries, fixing implantable devices, haemostasis. However, most rely on the instant formation of irreversible covalent crosslinks to provide surface binding. Repositioning misplaced adhesives during surgical operations may cause severe secondary damage tissues. Here, we report hydrogel tapes that can form physical interactions with tissues seconds gradually bonds hours. This...
Abstract The complexity of the tumor microenvironment (TME) is a crucial factor in lung adenocarcinoma (LUAD) progression. To gain deeper insights into molecular mechanisms LUAD, we perform an integrative single-cell RNA sequencing (scRNA-seq) data analysis 377,574 cells from 117 LUAD patient samples. By linking scRNA-seq with bulk gene expression data, identify cluster prognostic-related UPP1 high cells. These cells, primarily situated at invasive front tumors, display stronger association...
It has been proposed that cells sense hypoxia by a heme protein, which transmits signal activates the heterodimeric transcription factor hypoxia-inducible 1 (HIF-1), thereby inducing number of physiologically relevant genes such as erythropoietin (Epo). We have investigated mechanism two heme-binding ligands, carbon monoxide and nitric oxide, affect oxygen sensing signaling. Two concentrations CO (10 80%) suppressed activation HIF-1 induction Epo mRNA in dose-dependent manner. In contrast,...
It is a desirable and powerful strategy to precisely fabricate functional soft matter through self-assembly of molecular building blocks across range length scales. Proteins, nucleic acids, polyphenols are the self-assemblers ubiquitous in nature. Assembly proteins into flexible biocolloids, amyloid fibrils with high aspect ratio, has emerged as an unchallenged templating for high-end technological materials bio-nanotechnologies. We demonstrate ability these support deposition hybrid...
Lung squamous cell carcinoma (LUSC) is associated with poor clinical prognosis and lacks available targeted therapy. Novel molecules are urgently required for the diagnosis of LUSC. Here, we conducted our data mining analysis LUSC by integrating differentially expressed genes acquired from Gene Expression Omnibus (GEO) database comparing tumor tissues versus normal (GSE8569, GSE21933, GSE33479, GSE33532, GSE40275, GSE62113, GSE74706) into The Cancer Genome Atlas (TCGA) which includes 502...
A new classification of pulmonary adenocarcinoma has been recently proposed by the International Association for Study Lung Cancer, American Thoracic Society and European Respiratory (IASLC/ATS/ERS). This study was undertaken in an attempt to explore clinical implication this Chinese patients.Two hundred ninety-two lung adenocarcinomas were reclassified strictly according IASLC/ATS/ERS two pathologists, independently. Kaplan-Meier Cox regression analyses used analyze correlation between...
MicroRNAs (miRNA) are involved in and controlled by epigenetic regulation, thereby form a reciprocal regulatory circuit. Using next-generation sequencing (NGS)-based miRNA profiling, this study aimed to discover esophageal squamous cell carcinoma (ESCC)-specific miRNAs miRNA-related modulations.NGS-based profiles were generated for four pairs of ESCC tissues adjacent normal tissues. In situ hybridization was used assess expression its correlation with prognosis. DNA methylations identified...
BACKGROUND:In developed countries, prostate cancer (PCa) is a frequently diagnosed with the second highest fatality rate. Circular RNAs (circRNAs) are class of endogenous non-coding (ncRNAs) stably expressed in cells and involved series carcinomas. However, few research studies have reported on role circRNAs PCa. MATERIAL AND METHODS:We used qRT-PCR to detect expression circMYLK (circRNA ID: hsa_circ_0141940) miR-29a PCa tissues cell lines. MTT, colony formation, TUNEL assays were performed...
In non-small cell lung cancer (NSCLC), both USP7 expression and p53 gene status were reported to be an indicator of poor prognosis in adenocarcinoma patients; however, its roles mechanisms squamous carcinoma large need clarified. The was examined NSCLC tumors (excluding adenocarcinoma), their corresponding non-tumorous tissues, cells. Then, the prognostic role analyzed 110 samples adenocarcinoma). Finally, proliferation, metastasis, invasion a assessed using specific vshRNA. higher tissues...
Predicting lung adenocarcinoma (LUAD) risk is crucial in determining further treatment strategies. Molecular biomarkers may improve stratification for LUAD.We analyzed the gene expression profiles of LUAD patients from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). We initially used three distinct algorithms (sigFeature, random forest, univariate Cox regression) to evaluate each gene's prognostic relevance. Survival related genes were then fitted into least absolute...
Metal coordination bonds are widely used as the dynamic cross-linkers to construct self-healing hydrogels. However, it remains challenging independently improve toughness of metal coordinated hydrogels without affecting stretchability and properties, all these features directly correlated with properties same bonds. In this work, using histidine–Zn2+ binding an example, we show that number (the sites in each cross-linking ligand) is important parameter for mechanical strength By increasing...
Significance Hydrogels are extensively used for cell culture, tissue engineering, and flexible electronics. In all of these applications, mechanical properties hydrogels play an important role. Although tremendous studies have been devoted to optimizing the stiffness, strain, toughness, dynamic response, homogeneity has rarely considered. By developing a general strategy control hydrogels, here we show that nanoscale variation in matrix stiffness can considerably affect lineage specification...
Abstract Biological load‐bearing tissues are strong, tough, and recoverable under periodic mechanical loads. However, such features have rarely been achieved simultaneously in the same synthetic hydrogels. Here, we use a force‐coupled enzymatic reaction to tune strong covalent peptide linkage reversible bond. Based on this concept engineered double network hydrogels that combine high strength recovery Specifically, found ligase, sortase A, can promote proteolysis of peptides force. The bond...
Hypoxia-inducible factor 1α (HIF1α) plays a pivotal role in embryogenesis, angiogenesis, and tumorigenesis. HIF1α-mediated transcription requires the coactivator p300, at least part, through interaction with cysteine- histidine-rich 1 domain of p300. To understand molecular basis this interaction, we have developed random mutagenesis screen yeast approach for efficient identification residues that are functionally critical protein interactions. As result, four (Leu-795, Cys-800, Leu-818,...
Pulmonary infection is the most common risk factor for acute lung injury (ALI). Innate immune responses induced by Microbe-Associated Molecular Pattern (MAMP) molecules are essential defense but can lead to tissue injury. Little known about how MAMP degraded in or degradation/inactivation helps prevent ameliorate harmful inflammation that produces ALI. Acyloxyacyl hydrolase (AOAH) a host lipase inactivates Gram-negative bacterial endotoxin (lipopolysaccharide, LPS). We report here alveolar...
Paclitaxel (PTX) is the front‑line chemotherapeutic agent against human non‑small cell lung cancer (NSCLC). However, its therapeutic efficacy restricted by increasing frequency of resistance in NSCLC. Accumulating evidence has shown potential role microRNAs (miRNAs) sensitivity cells. Previously it was reported that microRNA‑7 (miR‑7) acts as an important tumor suppressor Therefore, present study conducted to determine regulatory miR‑7 PTX chemotherapy for Four NSCLC lines were used analyze...