A5085662152- Hepatitis C virus research
- Hepatitis B Virus Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Genetics, Aging, and Longevity in Model Organisms
- Neuropeptides and Animal Physiology
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- BRCA gene mutations in cancer
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Melanoma and MAPK Pathways
- Radiopharmaceutical Chemistry and Applications
- melanin and skin pigmentation
- Psoriasis: Treatment and Pathogenesis
- Cell death mechanisms and regulation
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Gene expression and cancer classification
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Bacterial Genetics and Biotechnology
- Genomics and Rare Diseases
Anti-Doping Laboratory
2023
Catholic Medical Center
2021
Mercy Catholic Medical Center
2021
Sidra Medical and Research Center
2016-2020
Qatar Airways (Qatar)
2018-2020
Mayo Clinic Hospital
2019
Phoenix (United States)
2019
Weill Cornell Medical College in Qatar
2012-2017
Cornell University
2013-2015
Brown University
2012-2013
The stress hormone epinephrine is known to elicit multiple systemic effects that include changes in cardiovascular parameters and immune responses. However, information about its direct action on cancer cells limited. Here we provide evidence reduces sensitivity of apoptosis through interaction with beta(2)-adrenergic receptors. antiapoptotic mechanism primarily involves phosphorylation inactivation the proapoptotic protein BAD by cAMP-dependent kinase. Moreover, was observed at...
Repertoire composition, quantity, and qualitative functional ability are the parameters that define virus-specific T-cell responses linked with their potential to control infection. We took advantage of segregation different hepatitis B virus (HBV) genotypes in geographically genetically distinct host populations directly analyze impact variables exert on these parameters. against entire HBV proteome were analyzed a total 109 HBV-infected subjects ethnicities (47 Chinese origin 62 Caucasian...
Cancer immune evasion is one of the principal mechanisms leading to progression and metastatization disease. Despite migration infiltration at tumor site cells, multiple factors can influence composition hot or "immune-sensitive" tumors cold "immune-resistant" tumors. Among responsible for make-up microenvironment are expression levels major histocompatibility molecules (MHC) antigen processing machinery, metabolic network, hypoxia, secretion pro-inflammatory (e.g., cytokines, chemokines,...
ABSTRACT Virus-specific CD8 T cells are activated when their T-cell receptors (TCRs) recognize the specific viral peptide/major histocompatibility complex (MHC) class I (pMHC) complexes present on surface of infected cells. Antibodies able to pMHC can mimic TCR specificity and both represent a valuable biological tool visualize serve as delivery system for highly targeted therapies. To evaluate these possibilities, we created monoclonal antibody specifically hepatitis B virus (HBV) envelope...
During antiviral therapy, specific delivery of interferon-α (IFNα) to infected cells may increase its efficacy, trigger a localized immune reaction, and reduce the side effects caused by systemic administration. Two T-cell receptor-like antibodies (TCR-L) able selectively bind hepatitis B virus (HBV)-infected hepatocytes chronic patients recognize core (HBc18-27) surface (HBs183-91) HBV epitopes associated with different human leukocyte antigen (HLA)-A*02 alleles (A*02:01, A*02:02, A*02:07,...
Functional brown adipose tissue (BAT), involved in energy expenditure, has recently been detected substantial amounts adults. Formerly overlooked BAT now become an attractive anti-obesity target.Molecular characterization of human and white adipocytes, using a myriad techniques including high-throughput RNA sequencing functional assays, showed that PAZ6 SW872 cells exhibit classical molecular phenotypic markers respectively. However, the pre-adipocyte cell line SGBS presents versatile...
Protection from apoptosis by receptor tyrosine kinases, resistant to the inhibition of phosphatidylinositol 3 '-kinase/Akt and Ras/MEK pathways, has been reported in several cell types, including fibroblasts epithelial prostate cancer cells; however, mechanisms this effect were not clear. Here we report that cells, epidermal growth factor activates two antiapoptotic signaling pathways impinge on proapoptotic protein BAD. One cascade operates via module induces BAD phosphorylation Ser112....
It has been demonstrated that vasoactive intestinal polypeptide, epidermal growth factor, and chronic activation of phosphatidylinositol 3-kinase can protect prostate cancer cells from apoptosis; however, the signaling pathways they use molecules target are unknown. We report activate independent phosphorylate proapoptotic protein BAD. Vasoactive polypeptide operated via kinase A, factor required Ras activity, effects were predominantly mediated by Akt. BAD phosphorylation was critical for...
Abstract Although the linkage between germline mutations of BRCA1 and hereditary breast/ovarian cancers is well established, recent evidence suggests that altered expression wild-type might contribute to sporadic forms breast cancer. The cancer gene trinucleotide-repeat-containing 9 (TNRC9; TOX3) has been associated with disease susceptibility but its function undetermined. Here, we report TNRC9 often amplified overexpressed in cancer, particularly advanced Gene amplification was reduced...
Transcriptome profiling approaches have been widely used to investigate the mechanisms underlying psoriasis pathogenesis. Most researchers measured changes in transcript abundance skin biopsies; relatively few examined transcriptome blood. Although less relevant study of pathogenesis, blood profiles can be readily compared across various diseases. Here, we a pre-established set 382 transcriptional modules as common framework compare two independent public datasets. We then resulting...
Oxidative stress is known to induce melanocyte death, but the underlying mechanisms are incompletely understood. To identify oxidative stress-induced global gene expression changes in melanocytes, we treated PIG1 melanocytes with H2O2 a dose- and time-dependent manner performed RNA-seq. This approach allowed us capture events occurring early as well late phase after treatment H2O2. Our bioinformatics analysis identified differentially expressed genes involved various biological processes of...
KCNH1 encodes a voltage-gated potassium channel that is predominantly expressed in the central nervous system. Mutations this gene were recently found to be responsible for Temple-Baraitser Syndrome (TMBTS) and Zimmermann-Laband syndrome (ZLS). Here, we report new case of TMBTS diagnosed Lebanese child. Whole genome sequencing was carried out on DNA samples proband his parents identify mutations associated with disease. Sanger performed confirm presence detected variants. revealed three...
Cancer stem cells (CSCs) are increasingly considered to be responsible for tumor initiation, metastasis and drug resistance. The resistance mechanisms activated in CSCs have not been thoroughly investigated. Although neuropeptides such as vasoactive intestinal peptide (VIP) can promote growth activate antiapoptotic signaling differentiated cancer cells, it is known whether they CSCs. objectives of this study unravel the cytoprotective effects identify by response anticancer treatment We...
A simple protective antigen (PA)-reactive mAb dot-ELISA was standardized for confirmation of toxin-producing strains Bacillus anthracis. Twenty-seven clinical isolates were collected from patients clinically suspected having anthrax. PA elaborated these using Casamino acids medium and the culture boiled to kill cells. in supernatants detected a dot-ELISA. Of 27 tested, 24 isolates. This further confirmed by amplifying gene PCR. testing procedure is perform, specific safer than existing...
Abstract Background Most mutations in melanoma affect one critical amino acid on BRAF gene, resulting the V600E substitution. Patient management is often based use of specific inhibitors targeting this mutation. Methods DNA and RNA mutation status was assessed 15 cell lines by Sanger sequencing RNA-seq. We tested responsiveness to (vemurafenib PLX4720, BRAF-specific sorafenib, non-specific). Cell proliferation MTT colorimetric assay. expression qPCR. Expression level phosphorylated-ERK...
The debate regarding whether to perform an interbody fusion after anterior cervical discectomy (ACD) has been going on for the last 50 years. Several prospective randomized clinical trials have performed evaluate outcome following with and without fusion. None of these studies show a significant difference between two techniques in terms relief arm pain or neck pain. operative time, in-hospital stay time return work seem be slightly longer grafting (ACDF) compared ACD alone. However...