Two important goals in orthopedic implant research are to promote osseointegration and prevent infection. However, much previous effort has been focused on the design of coatings either enhance while ignoring antibacterial activity or vice versa, infection bone integration. Here, we designed a multifunctional mineralized collagen coating titanium with aid metal-organic framework (MOF) nanocrystals control release naringin, Chinese herbal medicine that could bacterial The attachment,...

Rationale: For intravascular stent implantation to be successful, the processes of vascular tissue repair and therapy are considered critical. However, mechanisms underlying eventual fate smooth muscle cells (VSMCs) during remains elusive. In this study, we hypothesized that M2 macrophage-derived exosomes mediate cell-to-cell crosstalk induce dedifferentiation phenotypes in VSMCs. Methods:In vivo, 316L bare metal stents (BMS) were implanted from left iliac artery into abdominal aorta...

The binding of cell integrins to proteins adsorbed on the material surface is a highly dynamic process critical for guiding cellular responses. However, temporal regulation meet spatial conformation requirement certain response remains great challenge. Here, an active CoFe2O4/poly(vinylidene fluoride-trifluoroethylene) nanocomposite film, which was demonstrated be obvious potential variation (Δ V ≈ 93 mV) in applied magnetic field intensity (0-3000 Oe), designed harness integrin-adsorbed by...

Strontium (Sr) has shown strong osteogenic potential and thereby been widely incorporated into dental orthopedic implants. However, the improved osseointegration of strontium-modified titanium implants through regulation angiogenesis macrophage polarization is still beginning to be explored. Here, we demonstrated that angiogenic capacity human umbilical vein endothelial cells on Sr-incorporated micro/nano (SLA-Sr) surface was also significantly up-regulated expression HIF-1α protein Erk1/2...

Electrical stimulation (ES) has been considered a promising strategy in regulating intracellular communication, membrane depolarization, ion transport, etc. Meanwhile, cell topography, such as the alignment and elongation anisotropic orientation, also plays critical role triggering mechanotransduction well cellular fate. However, coupling of ES orientation to regulate polarization macrophages is yet be explored. In this work, we intended explore on poly(vinylidene fluoride-trifluoroethylene...

Macrophages have two functionalized phenotypes, M1 and M2, the regulation of M1/M2 polarization macrophages is critical for tissue repair. Tissue-derived immune factors are considered major drivers macrophage polarization. Based on main cytokine-induced pathways, we tested effect electrical stimulation (ES) a possible synergistic with cytokines. Indium tin oxide (ITO) planar microelectrodes were used to produce ES under different voltages, frequencies waveforms. We evaluated influence using...

The reciprocal connection between cell migration and proliferation relies on the intertwined contributions from substrate-associated intercellular cues in microenvironment. However, how cells perceive substrates, make contact with their neighbors, switch phenotypes under different trade-off conditions are still not fully understood. Here, we designed a distinct heterogeneous electric surface potential gradient of piezoelectric biomaterials to decouple density-dependent...

Well-organized orientation of cells and anisotropic extracellular matrix (ECM) are crucial in engineering biomimetic tissues, such as muscles, arteries, nervous system, so on. This strategy, however, is only beginning to be explored. Here, we demonstrated a light-induced cell alignment harvest for sheets (ACS) technology using light-responsive TiO2 nanodots film (TNF) photo-cross-linkable gelatin methacrylate (GelMA). Cell initial behaviors on TNF might controlled by micropatterns distinct...

The comparative quantity of the distinct type surface hydroxyl groups (bridging-OH and terminal-OH) plays key role in cell–material interactions.

In electrical stimulation (ES), daily time means the interacting duration with cells per day, and is a vital factor for mediating cellular function. present study, effect of on osteogenic differentiation MC3T3-E1 was investigated under ES polypyrrole (Ppy) planar interdigitated electrodes (IDE). The results demonstrated that only suitable supported to obviously upregulate expression ALP protein osteogenesis-related genes (ALP, Col-I, Runx2 OCN), while short or long showed no significant...

The surface electric potential of biomaterials has been extensively proven to play a critical role in stem cells' fate. However, there are ambiguous reports on the relation osteogenic capacity characteristics (potential polarity and intensity). To address this, we adopted with wide range both positive/negative comprehensive view get insight into potential-regulating cellular differentiation. Tb xDy1- xFe2 alloy/poly(vinylidene fluoride-trifluoroethylene) magnetoelectric films were prepared,...

Precisely controlling the protein-nanomaterial interactions at selective sites is crucial in engineering biomolecule composite architectures with tailored nanostructures and functions for a variety of biomedical applications. This strategy, however, only beginning to be explored. Here, we demonstrate facet-specific assembly proteins, such as albumin, immunoglobulin protamine, on {100} facets SrTiO3 polyhedral nanocrystals, while none {110} facets. Molecular dynamics simulations indicate...

Naringin, a Chinese herbal medicine, has been demonstrated to concentration-dependently promote osteogenic differentiation of mesenchymal stem cells (MSCs). However, it remains challenge load naringin on coatings for osteogenesis and further control the release kinetics. Here, we that behavior rutile nanorod films could be controlled by either mixing with gelatin methacryloyl (GelMA) before spinning onto or soaking obtained GelMA-incorporated solution achieve distinct degradation-type...

The immune response of bone implants is closely related to the interaction between macrophages and biomaterial surfaces.

Abstract Piezoelectric biomaterials are considered to be able mimic the electrophysiological microenvironment of natural bone tissue, thus enhancing regeneration. However, effects heterogeneous electric potential gradient piezoelectric on their osteogenic performance still remain elusive, largely because challenge harassing distribution surface biomaterials. This study controls CoFe 2 O 4 /poly(vinylidene fluoride‐trifluoroethylene (CFO/P(VDF‐TrFE)) membrane by using an alternatively...

Well-aligned collagen nanofibers are crucial in engineering bioinspired regenerative strategies, such as bone, muscle and cornea. However, keeping the natural bioactive of controlling its orientation a coating still remain challenge. Here we present novel magnetically assisted electrochemical technique to deposit type-I with high alignment onto titanium. The magnetic assistance involved mainly incorporation iron oxide nanoparticles (IOPs) application an external field during deposition....

Stem cells in contact with materials are able to sense their surface features, integrate extracellular matrix (ECM) protein cues through a signal transduction pathway, and ultimately direct cell fate decisions. However, discovering the interdisciplinary mechanisms of how stem respond inherent material features still remains challenge due complex, multicomponent signaling milieu present ECM environment. Here, we demonstrate that human mesenchymal (hMSCs) can be regulated by physical cue down...

Abstract Electrical stimulation has been proved to be critical regulate cell behavior. But, behavior is also susceptible multiple parameters of the adverse interferences such as surface current, electrochemical reaction products, and non‐uniform compositions, which often occur during direct electric stimulation. To effectively prevent interferences, a novel piezoelectric poly(vinylidene fluoride‐trfluoroethylene)(P(VDF‐TrFE)) layer was designed coat onto indium tin oxide (ITO) planar...