A5090798289- DNA Repair Mechanisms
- MicroRNA in disease regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Effects of Radiation Exposure
- Metal-Organic Frameworks: Synthesis and Applications
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Crystal structures of chemical compounds
- Cancer-related molecular mechanisms research
- Metal complexes synthesis and properties
- Microtubule and mitosis dynamics
- Cancer Research and Treatments
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Circular RNAs in diseases
- Fungal and yeast genetics research
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- interferon and immune responses
- Plant biochemistry and biosynthesis
- Fermentation and Sensory Analysis
- Occupational and environmental lung diseases
- Viral-associated cancers and disorders
- Cancer-related gene regulation
Zhejiang Chinese Medical University
2025
Beijing Radiation Center
2013-2024
University of South China
2019-2024
Hebei University
2024
Shihezi University
2004-2023
Beijing Hospital
2020
Hengyang Academy of Agricultural Sciences
2019
Academy of Military Medical Sciences
2018
Beijing Proteome Research Center
2016
Indiana University School of Medicine
2013
Abstract Background DNA double-strand break (DSB) induction and repair are important events for determining cell survival the outcome of cancer radiotherapy. The DNA-dependent protein kinase (DNA-PK) complex functions at apex DSBs repair, its assembly activity strictly regulated by post-translation modifications (PTMs)-associated interactions. However, PTMs catalytic subunit DNA-PKcs how they affect DNA-PKcs’s not fully understood. Methods Mass spectrometry analyses were performed to...
Abstract The ubiquitin ligase Smad ubiquitination regulatory factor-1 (Smurf1) negatively regulates bone morphogenetic protein (BMP) pathway by ubiquitinating certain signal components for degradation. Thus, it can be an eligible pharmacological target increasing BMP responsiveness. We established a strategy to discover small molecule compounds that block the WW1 domain of Smurf1 from interacting with Smad1/5 structure based virtual screening, molecular experimental examination and...
Abstract Radiotherapy is essential for treating colorectal cancer (CRC), especially in advanced rectal cancer. However, the low radiosensitivity of CRC cells greatly limits radiotherapy efficacy. Small nucleolar RNAs (snoRNAs) are a class noncoding RNA that primarily direct post‐transcriptional modifications ribosomal (rRNAs), small nuclear (snRNAs), and other cellular RNAs. While snoRNAs involved tumor progression chemoresistance, their association with remains largely unknown. Herein,...
In eukaryotic cells, DNA replication is carried out by coordinated actions of many proteins, including polymerase delta (pol delta), factor C (RFC), proliferating cell nuclear antigen (PCNA) and protein A. Here we describe dynamic properties these proteins in the elongation step on a single-stranded M13 template, providing evidence that pol has distributive nature over 7 kb repeating frequent dissociation-association cycle at growing 3'-hydroxyl ends. Some PCNA could remain primer terminus...
Radiation-induced pulmonary fibrosis (RIPF) results from thoracic radiotherapy and severely limits the use of radiotherapy. Recent studies suggest that epithelium-to-mesenchymal transition (EMT) contributes to fibrosis. Although miRNA dysregulation participates in a variety pathophysiologic processes, their roles fibrotic lung diseases EMT are unclear. In this study, we aimed identify key miRNAs involved process using mouse model RIPF previously established by irradiation with single dose...
Ionizing radiation (IR) can induce pulmonary fibrosis by causing epithelial mesenchymal transition (EMT), but the exact mechanism has not been elucidated. To investigate molecular of how induces altering miR-486-3p content and thus inducing EMT.The changes in cells after irradiation were detected RT-qPCR. Western blot was used to detect cellular marker protein E-cadherin, N-cadherin, Vimentin other proteins. The target gene predicted bioinformatics method binding site verified dual...
Abstract Introduction Radiation‐induced pulmonary fibrosis (RIPF) is a chronic, progressive, irreversible lung interstitial disease that develops after radiotherapy. Although several previous studies have focused on the mechanism of epithelial–mesenchymal transition (EMT) in epithelial cells, essential factors involved this process remain poorly understood. The DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) exhibits strong repair capacity when cells undergo radiation‐induced...
Understanding miRNAs regulatory roles in epithelial-mesenchymal transition (EMT) would help establish new avenues for further uncovering the mechanisms underlying radiation-induced pulmonary fibrosis (RIPF) and identifying preventative therapeutic targets. Here, we demonstrated that miR-541-5p repression by Myeloid Zinc Finger 1 (
DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is the core component of DNA-PK complex in non-homologous end-joining (NHEJ) repair DNA double-strand breaks, and its activity strictly controlled by DNA-PKcs phosphorylation. The ubiquitin-like protein, NEDD8 involved regulation damage response, but it remains mysterious whether how NEDD8-related neddylation affects NHEJ process. Here, we show that poly-neddylated at domain. E2-conjugating enzyme UBE2M E3 ligase HUWE1 (HECT, UBA, WWE...
DNA‑dependent protein kinase catalytic subunit (‑PKcs) is the core involved in non‑homologous end‑joining repair of double‑strand breaks. In addition, it can form a complex with poly(ADP‑ribose) polymerase 1 (PARP1), which catalyzes PARylation. However, unclear how DNA‑PKcs interacts PARP1 DNA damage response and PARylation affects DNA‑PK activity. Using immunoprecipitation, immunofluorescence flow cytometry present study found that was PARylated after damage, PARP1/2 inhibitor olaparib...
Abstract Whole-body exposure to low-dose radiation due diagnostic imaging procedures, occupational hazards and accidents is a source of concern. In this study, we analyzed the effects single long-term irradiation on immune system. Male Balb/c mice received whole-body dose (0.01, 0.05, 0.2, 0.5 or 1 Gy). For irradiation, were irradiated 10 times (total Gy) over period 6 weeks. Two days after numbers splenic macrophages, natural killer cells dendritic reduced, spleen organ coefficient was...
The evolutionary conserved PIF1 DNA helicase family appears to have largely nonoverlapping cellular functions.To better understand the functions of human PIF1, we investigated biochemical properties this protein.Analysis single-stranded (ss) DNAdependent ATPase activity revealed nonstructural ssDNA greatly stimulate due a high affinity for even though preferentially unwinds forked substrates.This suggests that needs region loading and structure translocation entrance into double strand...
The HECT-type ubiquitin ligase Smurf1 (Smad ubiquitination regulatory factor-1) plays the prominent role in regulation of bone formation, embryonic development, and tumorigenesis by directing ubiquitin-proteasomal degradation specific targets. In contrast with RING-type E3s, catalytic HECT domain firstly binds to then transfers (Ub) molecules onto substrates. Smurf1-Ub interaction is required for activity promote substrate degradation. However, so far regulators or compounds controlling have...
Colorectal cancer (CRC) is one of the most common malignant tumors digestive system, and represents a severe threat to life health individuals. Increasing evidence supports role small nucleolar RNAs (snoRNAs) as critical regulatory gene in development. Small Cajal body-specific (scaRNAs), subtype snoRNAs, are named for their subcellular localization within bodies. SCARNA12, which located at intronic region PHB2 chromosome 12p13.31 with 270 nucleotides (nt) length. It has been reported...
Although recent studies provide mechanistic understanding to the pathogenesis of radiation induced lung injury (RILI), rare therapeutics show definitive promise for treating this disease. Type II alveolar epithelial cells (AECII) in various manner results an inflammation response initiate RILI.
Thyroid cancer incidence increases worldwide annually, primarily due to factors such as ionizing radiation (IR), iodine intake, and genetics. Papillary carcinoma of the thyroid (PTC) accounts for about 80% cases. RET/PTC1 (coiled-coil domain containing 6 [CCDC6]-rearranged during transfection) rearrangement is a distinctive feature in over 70% cancers who exposed low doses IR Chernobyl Hiroshima‒Nagasaki atomic bombings. This study aims elucidate mechanism between PTC. N-thy-ori-3-1 cells...
Abstract N‐α‐Acetyltransferase 10 (NAA10) was reported to be involved in tumour invasion and metastasis several of tumours. However, the role mechanism NAA10‐mediated oral squamous cell carcinoma (OSCC) remains undetermined. Herein, our study showed that NAA10 inhibits migration vitro attenuates xenograft tumorigenesis nude mice. Mechanistically, we demonstrated there is a physical interaction between RelA/p65 OSCC cells, thereby preventing RelA/p65‐mediated transcriptional activation Pirh2....
Salt-inducible kinase 2 (SIK2) belongs to the serine/threonine protein kinases of AMPK/SNF1 family, which has important roles in cell cycle, tumor, melanogenesis, neuronal damage repair and apoptosis. Recent studies showed that SIK2 regulates macrophage polarization make a balance between inflammation macrophage. Macrophage is critical initiate immune regulation, however, whether can be involved regulation not still well understood. Here, we revealed was highly expressed thymus, spleen,...