A5010959343- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- Crystallization and Solubility Studies
- Surgical Sutures and Adhesives
- Hemostasis and retained surgical items
- X-ray Diffraction in Crystallography
- Synthesis and Biological Evaluation
- DNA Repair Mechanisms
- Wound Healing and Treatments
- Metal complexes synthesis and properties
- Protein Tyrosine Phosphatases
- Plant and animal studies
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Crystal structures of chemical compounds
- Electrospun Nanofibers in Biomedical Applications
- Anesthesia and Neurotoxicity Research
- Cancer, Hypoxia, and Metabolism
- Catalytic C–H Functionalization Methods
- Venous Thromboembolism Diagnosis and Management
- Conducting polymers and applications
- Protein Degradation and Inhibitors
Zhejiang Sci-Tech University
2016-2025
University of South China
2022-2024
Sun Yat-sen University
2023-2024
Sun Yat-sen University Cancer Center
2023-2024
Sichuan University
2013-2024
West China Hospital of Sichuan University
2017-2024
Beijing Radiation Center
2022-2023
State Key Laboratory of Oncology in South China
2023
University of California, San Francisco
2021-2022
Kunming Medical University
2022
In the search for biocompatible composite microspheres to be used as a hemostatic agent, in previous study, we designed novel biomaterial, consisting of containing three natural biological ingredients, carboxymethyl chitosan, sodium alginate and collagen (CSCM). Furthermore, chemical physical properties, ability, biocompatibility cytotoxicity were investigated vitro. this work, vivo performance, wound healing, hemocompatibility, histocompatibility, biodegradability evaluated by series...
Abstract Background DNA double-strand break (DSB) induction and repair are important events for determining cell survival the outcome of cancer radiotherapy. The DNA-dependent protein kinase (DNA-PK) complex functions at apex DSBs repair, its assembly activity strictly regulated by post-translation modifications (PTMs)-associated interactions. However, PTMs catalytic subunit DNA-PKcs how they affect DNA-PKcs’s not fully understood. Methods Mass spectrometry analyses were performed to...
To develop biocompatible composite microspheres for novel hemostatic use, we designed and prepared a biomaterial, consisting of carboxymethyl chitosan, sodium alginate, collagen (CSCM). The ultra-structure CSCM was investigated by scanning electron microscopy assay. In function experiment, it found that could facilitate platelet adherence, aggregation, activation in vitro. Besides, the maximum swelling submerged PBS 50 min over 300% exhibited commercial compound microporous polysaccharide...
Background and Objective: Epigenetic alterations are common events in clear cell renal carcinoma (ccRCC), protein arginine methyltransferase 1 (PRMT1) is an important epigenetic regulator cancers. However, its role ccRCC remains unclear. Methods: We investigated PRMT1 expression level correlations to clinicopathological factors prognosis patients based on tissue microarrays (TMAs). Genetic knockdown pharmacological inhibition using a novel inhibitor DCPT1061 were performed investigate the...
Many neurodegenerative diseases such as Alzheimer's disease are characterized by pathological β-sheet filaments of the tau protein, which spread in a prion-like manner patient brains. To date, high-resolution structures obtained from brains show that core only includes portions microtubule-binding repeat domains and excludes C-terminal residues, indicating C-terminus is dynamically disordered. Here, we use solid-state NMR spectroscopy to identify full-length 0N3R fibrillized using heparin....
Due to the rise in bacterial resistance, antibacterial extractions from Chinese herbs have been used more frequently for wound care. In this work, baicalin, an extraction herb Scutellaria baicalensis, was utilized as component poly(ε-caprolactone)/MXene (PCL/Ti3C2TX) hybrid nanofibrous membranes dressing. The results revealed that presence of Ti3C2TX aided diameter reduction electrospun nanofibers. PCL membrane containing 3 wt% nanoflakes and 5 baicalin exhibited smallest mean 210 nm....
Non-isothermal crystallization and subsequent melting of three grades ethylene-vinyl acetate copolymer were investigated by differential scanning calorimetry (DSC) technique.The DSC curves show that vinyl (VAc) content has the same effect on onset, peak final temperatures, these copolymers have almost spacing thermal windows under identical condition.Subsequent results suggest EVA14 (14 wt% VAc) narrowest distribution lamellar thickness most perfect crystals.Though instantaneous nucleation...
Significance Mutations in a microglial protein, TREM2, represents risk for Alzheimer’s disease (AD). We show that the soluble form of sTREM2, can bind and inhibit fibrillization Aβ peptides. sTREM2 increases uptake fibrils into neuroglioma cell lines. Contrastingly, mutation R47H was found to have little effect on fibril nucleation binding, but decreased functional responses. Our findings using integrative molecular modeling based cross-linking mass spectrometry data WT sTREM2–Aβ complex...
In castration-resistant prostate cancer (CRPC), clinical response to androgen receptor (AR) antagonists is limited mainly due AR-variants expression and restored AR signaling. The metabolite spermine most abundant in it decreases as progresses, but its functions remain poorly understood. Here, we show inhibits full-length (AR-FL) splice variant 7 (AR-V7) signaling suppresses CRPC cell proliferation by directly binding inhibiting protein arginine methyltransferase PRMT1. Spermine reduces...
The alkylation of nitro compounds is an efficient technique to augment molecular complexity, as exemplified by the Henry reaction and Michael addition involving sp2-hybridized carbon. However, a challenge arises in with sp3-hybridized carbon due competing nonproductive O-alkylation. In this study, we have developed manganese-promoted oxidative nitroalkanes using cyclopropyl alcohols via solvent-caged free-radical nucleophilic substitution pathway. This process yields variety γ-nitroketones,...
Protein Arginine Methyltransferases (PRMTs) are crucial players in diverse biological processes, and dysregulation of PRMTs has been linked to various human diseases, especially cancer. Therefore, small molecules targeting have profound impact for both academic functional studies clinical disease treatment. Here, we report the discovery N1-(2-((2-chlorophenyl)thio)benzyl)-N1-methylethane-1,2-diamine (28d, DCPR049_12), a highly potent inhibitor type I that good selectivity against panel other...
Coactivator-associated arginine methyltransferase 1 (CARM1), an important member of type I protein methyltransferases (PRMTs), has emerged as a promising therapeutic target for various cancer types. In our previous study, we have identified series PRMT inhibitors, among which ZL-28-6 (
A series of novel hyperbranched ladder-type poly(p-phenylene)s containing truxene units have been prepared through the Pd(0)-catalyzed Suzuki polymerization with an "A2 + B3" or B2+ approach. The polymers different linear length show pure blue light emission from 417 to 465 nm. All exhibit well-resolved absorption and spectra small Stokes shifts in both solutions thin films, which are characteristics full poly(p-phenylene)s. three-dimensional structures make materials soluble common organic...
Biomimetic microspheres containing alginate/carboxymethylcellulose/gelatin and coated with 0%, 1%, 3%, 6% berberine (BACG, BACG-1B, BACG-3B, BACG-6B) were prepared by the oil-in-water emulsion method combined spray drying. Through a series of physicochemical parameters determination hemostatic properties in vitro vivo, results indicated that BACG BACG-Bs effective inducing platelet adhesion/aggregation promoting potential due to their biomimetic structure rough surface. In addition, BACG-6B...
Light transmission aggregometry (LTA) is the "gold standard" for platelet function assessment, but it time-consuming and labor intensive. Recently, an automated aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, performances of CS-2100i including repeatability, correlation with reference aggregometer (Chrono-log Model 700), threshold limitation counts in platelet-rich plasma (PRP) were evaluated clinical use. The agonists adenosine...
DNA methyltransferase-1 (DNMT1) plays a crucial role in the maintenance of genomic methylation patterns. The crystal structure DNMT1 was determined two different states which helix that follows catalytic loop either kinked (designated helix-kinked) or well folded helix-straight state). Here, we show proper structural transition between these is required for activity. mutations N1248A and R1279D, did not affect interactions substrates cofactors, allosterically reduced enzymatic activities...