A5100356730- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- Phosphodiesterase function and regulation
- Genomics, phytochemicals, and oxidative stress
- Bioactive natural compounds
- Phenothiazines and Benzothiazines Synthesis and Activities
- Ubiquitin and proteasome pathways
- Phytochemistry and Biological Activities
- Advanced Antenna and Metasurface Technologies
- Pharmaceutical and Antibiotic Environmental Impacts
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- Natural product bioactivities and synthesis
- Protease and Inhibitor Mechanisms
- Chemical synthesis and alkaloids
- Glycosylation and Glycoproteins Research
- S100 Proteins and Annexins
- Antibiotic Resistance in Bacteria
- Peptidase Inhibition and Analysis
- Electromagnetic wave absorption materials
- Protein Interaction Studies and Fluorescence Analysis
Harbin Medical University
2025
Tianjin University of Traditional Chinese Medicine
2020-2025
Changzhou University
2019-2024
Yangzhou University
2024
Zhejiang University of Technology
2024
Shanghai Jiao Tong University
2006-2023
Linyi University
2023
Taiyuan University of Technology
2023
Anhui Agricultural University
2023
North China University of Science and Technology Affiliated Hospital
2022
The NLRP3 inflammasome is a multiprotein complex that protects hosts against variety of pathogens. However, the molecular mechanisms modulating activation, especially at priming step, are still poorly understood. This study was designed to elucidate negative regulation nuclear factor E2-related factor-2 (Nrf2) on activation inflammasome.We reported Nrf2 inhibited expression, caspase-1 cleavage, and subsequent IL-1β generation. Compared with normal cells, Nrf2-deficient cells showed...
Accumulated evidence proves that mature spermatozoa contain a complex yet specific array of mRNA, which could provide information on the past events spermatogenesis.To quantitatively microdissect these mRNA transcripts by digital approach.Serial analysis gene expression (SAGE) was used to study from ejaculate fertile individual and pool 10 men. Online DAVID software suite also utilized cluster UniGene data.A SAGE library produced 20,237 raw tags representing 2459 unique pooled men generated...
Regulating inflammation could be an important measure for the effective treatment of cancer. Here we examine mechanisms by which oroxylin A inhibits in RAW264.7 cells. The results demonstrate that pretreatment with (50, 100, and 150 μmol/L) inhibited lipopolysaccharide (LPS)-induced mRNA protein expression COX-2 iNOS. In addition, significantly increased nuclear factor erythroid 2-related 2 (Nrf2), heme oxygenase 1 (HO-1), NADP(H):quinone oxidoreductase (NQO1), induced Nrf2 translocation to...
The pollen of T. angustifolia, also known as Pu huang in Chinese, has been used for treatment stranguria, hematuria, dysmenorrhea, metrorrhagia and injuries China a long time. Extensive efforts have directed toward its phytochemical biological aspects. However, little is about anti-nociceptive implication material basis. This work presented the investigation effect Typhae Pollen using an effect-directed fractionation strategy, thereby leading to identification isorhamnetin-3-O-neohesperidin...
Abstract The bark and the leaf of Eucommia ulmoides Oliv. content similar bioactive components, but this medically important plant is mostly abandoned. In study, we revealed that aqueous extract E. (EUL) can promote growth probiotic Lactobacillus bulgaricus (LB) inhibit formation osteoclast in vitro. This was next administrated to senescence‐accelerated mice P6 evaluate examine its influence on composition gut microbiota (GM), short‐chain fatty acids (SCFAs), osteoporosis (OP). results...
Nrf2-mediated activation of ARE regulates expression cytoprotective enzymes against oxidative stress, inflammation, and carcinogenesis. We have discovered a novel structure (1) as an inducer via luciferase reporter assay to screen the in-house database our laboratory. The potency 1 was evaluated by NQO-1, HO-1, nuclear translocation Nrf2 in HCT116 cells. In vivo studied using AOM-DSS models, showing that development colorectal adenomas significantly inhibited. Administration with lowered...
To investigate the clinical value of plasma cell-free DNA (cfDNA) as a potential biomarker for advanced gastric cancer (GC).One hundred and six cases patients receiving chemotherapy were selected study objects. Another 40 healthy volunteers included control groups. Plasma cfDNA concentration was detected by (SuperbDNATM) hybridization. Changes in during with whose efficacy assessed partial response (PR), stable disease (SD) progression (PD) analyzed respectively. The relationship between...
The present study aims at designing a thermosensitive gel prepared from w1/o/w2 multiple microemulsions (MMEs) for the vaginal delivery of siRNA. MMEs were by two-step emulsifications: first step was to prepare primary emulsions (w1/o) low energy emulsification (LEE); second obtain stable self-emulsifying. An extensive formulation optimization process undertaken. final could be formed in ddH2O, phosphate buffer solution (PBS, pH 7.4) and 1640 culture media with diameter size about 166.5 ±...
Nonresolving inflammation is one of the consistent features tumor microenvironment in intestine and plays a critical role initiation development colon cancer. Here we reported inhibitory effects GEN-27, new derivative genistein, on inflammation-related cancer cell proliferation delineated mechanism its action. The results indicated that GEN-27 inhibited human HCT116 cells stimulated by culture supernatants LPS-induced monocytes THP-1 significantly decreased secretion proinflammatory...
This study was undertaken to investigate drug resistance in Escherichia coli (E. coli) strains isolated from bamboo rats Zhejiang province of China. One hundred and fifty-four E. were dead rats. Polymerase chain reaction (PCR) used detect the representative genes encoding commonly β-lactam antibiotics. Highest observed for cefradine (24.03%), followed by penicillin (20.78%) ceftazidime (20.13%). The isolation rates 53.25, 48.70, 15.58 14.29% bla TEM, CTX-M, OXA SHV, respectively, while 62...
Inducing human-periodontal-ligament-stem-cell (hPDLSC) differentiation by DNA transfection is a promising way for periodontal tissue engineering. However, there are only few studies that focus on the introduction of foreign into hPDLSCs nonviral methods relatively safe. Hence, major purposes this study were to compare efficiency and toxicity nonviral-gene-transfer hPDLSCs, search best approach optimal protocol transferring genes using vectors. The transfected (1) Lipofectamine 2000, (2)...