A5030933195- Wound Healing and Treatments
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Corneal Surgery and Treatments
- Autophagy in Disease and Therapy
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Organ Transplantation Techniques and Outcomes
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- bioluminescence and chemiluminescence research
- Hepatitis B Virus Studies
- Collagen: Extraction and Characterization
- Liver physiology and pathology
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Cytomegalovirus and herpesvirus research
- Dermatologic Treatments and Research
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
Thermo Fisher Scientific (United States)
2016-2024
University of Pittsburgh
2016-2022
McGowan Institute for Regenerative Medicine
2022
VA Pittsburgh Healthcare System
2018-2022
Thermo Fisher Scientific (Israel)
2016
Hepatic repair is directed chiefly by the proliferation of resident mature epithelial cells. Furthermore, if predominant injury to cholangiocytes, hepatocytes can transdifferentiate cholangiocytes assist in and vice versa, as shown various fate‐tracing studies. However, molecular bases reprogramming remain elusive. Using two models biliary where occurs through cholangiocyte hepatocyte transdifferentiation we identify an important role Wnt signaling. First up‐regulation specific proteins...
Abstract Background Mesenchymal stem/multipotent stromal cells (MSCs) contribute to tissue repair but are challenged during wound healing when the blood supply is disrupted, thereby limiting nutrient delivery. Survival mechanisms against ‘starvation’ include autophagy, which we previously found enhance differentiation efficiency. MSC response models of in vitro deprivation great interest for improving survival and therapeutic efficacy; however, rate-limiting nutrients unknown. Methods...
Stem cells from human corneal stroma (CSSC) suppress stromal scarring in a mouse wound-healing model and promote regeneration of native transparent tissue (PMID:25504883). This study investigated efficacy compressed collagen gel (CCG) as vehicle to deliver CSSC for therapy. isolated limbal donor corneas were embedded soluble rat-tendon collagen, gelled at 37°C, partially dehydrated thickness 100 µm by passive absorption. The CCG disks dimensionally stable, easy handle, could be adhered...
Cutaneous wound healing is an intricate orchestration of three overlapping phases repair that encompass numerous cell types, signalling cascades, and microenvironment modifications to reach a successful resolution. Disruption any these steps will create abnormal response resulting in either ulceration or excessive scarring. It has become evident the extracellular matrix its associated components are key orchestrators during this process. One essential proteins decorin, small leucine-rich...
Somatic reprogramming has enabled the conversion of adult cells to induced pluripotent stem (iPSC) from diverse genetic backgrounds and disease phenotypes. Recent advances have identified more efficient safe methods for introduction factors. However, there are few tools monitor track progression reprogramming. Current monitoring rely on qualitative inspection morphology or staining with cell-specific dyes antibodies. Tools dissect iPSC generation can help better understand process under...
Human mesenchymal stem cells/multipotent stromal cells (MSCs) hold great promise in aiding wound healing through their ability to modulate all phases of repair and regeneration, most notably secretion pro-regenerative paracrine factors. However, MSC clinical utility is hindered by poor survival rates post-transplantation due the harsh microenvironment injured tissue. Previous work has shown that matricellular protein Tenascin-C (TNC) provides signaling MSCs via epidermal growth factor...
Somatic reprogramming has enabled the conversion of adult cells to induced pluripotent stem (iPSC) from diverse genetic backgrounds and disease phenotypes. Recent advances have identified more efficient safe methods for introduction factors. However, there are few tools monitor track progression reprogramming. Current monitoring rely on qualitative inspection morphology or staining with cell-specific dyes antibodies. Tools dissect iPSC generation can help better understand process under...
Abstract Even the most therapy responsive melanomas eventually evade immune checkpoint inhibition. Interestingly, initial responses seem to benefit from localized inflammation, but this can become prohibitive when treating advanced disease. Subsequently, there is an urgent need elucidate how inflammation impacts tumor microenvironment (TME). Here, inhibition of EGFR and Ras/Raf/MEK shown drive release a pro-inflammatory secretome that activates TME promote progression. This was enriched with...