A5101600516- Liver physiology and pathology
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- interferon and immune responses
- Pancreatic function and diabetes
- Organ Transplantation Techniques and Outcomes
- Cellular Mechanics and Interactions
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Gut microbiota and health
- Cell death mechanisms and regulation
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- 14-3-3 protein interactions
- Synthesis and biological activity
- Liver Disease Diagnosis and Treatment
Chengde Medical University
2025
University of Pittsburgh
1998-2025
Hubei University of Medicine
2012-2024
Intellia Therapeutics (United States)
2024
Army Medical University
2015-2023
Southwest Hospital
2023
Nanjing Medical University
2022-2023
University of Jinan
2022
Guangdong Medical College
2022
Biogen (United States)
2021
Mesenchymal stem cell (MSC)-derived exosomes have diverse functions in regulating wound healing and inflammation; however, the molecular mechanism of human umbilical cord MSC (hUCMSC)-derived burn-induced inflammation is not well understood. We found that burn injury significantly increased inflammatory reaction rats or macrophages exposed to lipopolysaccharide (LPS), tumor necrosis factor α (TNF-α) interleukin-1β (IL-1β) levels decreased IL-10 levels. hUCMSC-exosome administration...
Liver-specific β-catenin knockout (β- Catenin -LKO) mice have revealed an essential role of in metabolic zonation where it regulates pericentral gene expression and initiating liver regeneration (LR) after partial hepatectomy (PH), by regulating Cyclin-D1. However, what activity these events remains enigma. Here we investigate to extent activation is Wnt-signaling-dependent the potential cell source Wnts. We studied liver-specific Lrp5/6 KO ( Lrp Wnt-signaling was abolished hepatocytes while...
The phenotypes of vascular smooth muscle cells (vSMCs) comprise a continuum bounded by predominantly contractile and synthetic cells. Some evidence suggests that vSMCs can assume more phenotype in response to ischemic injury, but the mechanisms activate this phenotypic switch are poorly understood.To determine whether lactate, which increases regional ischemia, may promote vSMCs.Experiments were performed with had been differentiated from human induced pluripotent stem then cultured...
Hepatic repair is directed chiefly by the proliferation of resident mature epithelial cells. Furthermore, if predominant injury to cholangiocytes, hepatocytes can transdifferentiate cholangiocytes assist in and vice versa, as shown various fate‐tracing studies. However, molecular bases reprogramming remain elusive. Using two models biliary where occurs through cholangiocyte hepatocyte transdifferentiation we identify an important role Wnt signaling. First up‐regulation specific proteins...
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression primarily at the post-transcriptional level and play critical roles in a variety of physiological pathological processes. In this report, miR-141 was identified to repress HBV by screening miRNA expressing library synthetic mimics could also significantly suppress replication HepG2 cells. Bioinformatic analysis experiment assays indicate peroxisome proliferator-activated receptor alpha (PPARA) target hsa-miR-141...
Mechanical strain substantially influences tissue shape and function in various contexts from embryonic development to disease progression. Disruptions these processes can result congenital abnormalities short-circuit mechanotransduction pathways. Manipulating live tissues is crucial for understanding its impact on cellular subcellular activities, unraveling the interplay between mechanics cells. Existing tools, such as optogenetic modulation of strain, are limited small strains over...
Aims: Previous studies have revealed that the increased shedding of syncytiotrophoblast extracellular vesicles (STBM) may lead to preeclampsia (PE). We aimed identify proteins carried by STBM and their potential pathological roles in early-onset severe PE. Methods: In this study, we performed a differential proteomic analysis from PE patients, using iTRAQ isobaric tags 2D nano LC-MS/MS. were generated vitro explant culture method, then verified electron microscopy western blot analysis....
The NF-κB pathway plays key roles in HIV-1 replication and reactivation of latency. A regulator inhibiting activation may be a promising therapeutic strategy against HIV-1. Recently, NF-κB-interacting long noncoding RNA (NKILA) was identified to suppress the development different human cancers by IκB kinase (IKK)-induced phosphorylation activation, whereas relationship between NKILA is still unknown. Here, our results show that inhibits suppressing terminal repeat (LTR)-driven transcription...
Oligonucleotide drugs show promise to treat diseases afflicting millions of people. To address the need manufacture large quantities oligonucleotide therapeutics, novel convergent liquid-phase synthesis has been developed for an 18-mer drug candidate. Fragments containing tetra- and pentamers were synthesized assembled into without column chromatography, which had a similar impurity profile material made by standard solid-phase synthesis. Two fragments have at ∼3 kg/batch sizes four...
Hepatocellular cancer (HCC) is the third cause of death by worldwide. In current study we target β- catenin, an oncogene mutated and constitutively active in 20-30% HCCs, via a novel, cell permeable gamma guanidine-based peptide nucleic acid (γGPNA) antisense oligonucleotide designed against either transcription or translation start site human β-catenin gene. Using TOPflash, luciferase reporter assay, show that γGPNA targeting showed more robust activity liver tumor cells harbor gene...
Hepatocellular carcinoma (HCC), the third most common cause of cancer-related deaths worldwide, lacks effective medical therapy. Large subsets HCC demonstrate Wnt/β-catenin activation, making this an attractive therapeutic target. We report strategy and characterization a novel small-molecule inhibitor, ICG-001, known to affect Wnt signaling by disrupting β-catenin-CREB binding protein interactions. queried ZINC online database for structural similarity ICG-001 identified PMED-1 as lead...
We sought to identify a secreted biomarker for β-catenin activation commonly seen in hepatocellular carcinoma (HCC). By examination of our previously published genearray hepatocyte-specific knockout (KO) livers, we identified factors whose expression may be β-catenin-dependent. verified and secretion the leading factor HCC cells transfected with mutated (Hep3BS33Y)-β-catenin. Serum levels were next investigated mouse model gene (Ctnnb1) mutations eventually patients. Leukocyte cell-derived...
Oligonucleotides have emerged as valuable new therapeutics. Presently, oligonucleotide manufacturing consists in a series of stepwise additions until the full-length product is obtained. Deprotection phosphorus backbone before cleavage and deprotection (C&D) by ammonolysis necessary to control 3-(2-cyanoethyl) thymidine (CNET) impurity. In this study, we demonstrate that use piperazine scavenger acrylonitrile allows C&D be combined single step. This reduces solvent consumption, processing...