A5044122499- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Wound Healing and Treatments
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Sirtuins and Resveratrol in Medicine
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Mesenchymal stem cell research
- S100 Proteins and Annexins
- Burn Injury Management and Outcomes
- Cardiac Arrest and Resuscitation
- Ubiquitin and proteasome pathways
- Chronic Kidney Disease and Diabetes
- Extracellular vesicles in disease
- Thyroid Disorders and Treatments
- Traditional Chinese Medicine Analysis
- Genetic and Kidney Cyst Diseases
- Cardiac electrophysiology and arrhythmias
- Vitamin C and Antioxidants Research
- Thermal Regulation in Medicine
- Peroxisome Proliferator-Activated Receptors
- Pregnancy and preeclampsia studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Chongqing Medical University
2024-2025
Army Medical University
2014-2024
Southwest Hospital
2014-2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2024
Daping Hospital
2010
Abstract Excessive activation of inflammation and the accompanying lung vascular endothelial barrier disruption are primary pathogenic features acute injury (ALI). Microtubule-associated protein 4 (MAP4), a tubulin assembly-promoting protein, is important for maintaining microtubule (MT) cytoskeleton cell-cell junctional structures. However, both involvement exact mechanism MAP4 in development ALI remains unknown. In this study, lipopolysaccharide (LPS) tumour necrosis factor-α (TNF-α) were...
Wound healing is delayed frequently in patients with diabetes. Proper keratinocyte migration an essential step during re-epithelialization. Impaired a critical underlying factor responsible for the deficiency of diabetic wound healing, which mainly attributed to hyperglycemic state. However, mechanisms remain largely unknown. Previously, we demonstrated marked activation p38/mitogen-activated protein kinase (MAPK) pathway regenerated migrating epidermis, turn promoted migration. In present...
Abstract BNIP3 is an atypical BH3-only member of the Bcl-2 family with pro-death, pro-autophagic, and cytoprotective functions, depending on type stress cellular context. Recently, we demonstrated that stimulates migration epidermal keratinocytes under hypoxia. In present study found autophagy expression were concomitantly elevated in migrating epidermis during wound healing a hypoxia-dependent manner. Inhibition through lysosome-specific chemicals (CQ BafA1) or Atg5-targeted...
Objectives This cross-sectional study aims to evaluate the association between calcium and vitamin D supplementation for osteoporosis treatment development of kidney stones while investigating impact urinary excretion on stone risk among patients receiving this treatment. Design The involved collecting data from 204 Chinese Han aged 50–89 with in southwest region China. These had been daily doses 600 mg carbonate 0.5 µg alfacalcidol at least 1 year. employed univariate analysis multivariable...
This study investigated the roles of Rho protein in epidermal growth factor (EGF)-induced trophoblast cell migration and its mechanism. Using choriocarcinoma lines JEG-3 JAR first-trimester human chorionic villus explant cultures on matrigel, we examined EGF-mediated stimulation migration. EGF is shown to have a dose-dependent effect A low concentration (1 ng/ml) has stimulatory migration, whereas high concentrations (100 shows an inhibitory effect. activates RhoA RhoC, but not RhoB, through...
Aims: Previous studies have revealed that the increased shedding of syncytiotrophoblast extracellular vesicles (STBM) may lead to preeclampsia (PE). We aimed identify proteins carried by STBM and their potential pathological roles in early-onset severe PE. Methods: In this study, we performed a differential proteomic analysis from PE patients, using iTRAQ isobaric tags 2D nano LC-MS/MS. were generated vitro explant culture method, then verified electron microscopy western blot analysis....
BackgroundCardiac remodeling is a pathophysiological process that involves various changes in heart, including cardiac hypertrophy and fibrosis. Cardiac following pathological stimuli common trigger leading to maladaptation onset of heart failure, their pathogenesis remains unclear.MethodsHeart specimens tetralogy Fallot (TOF) patients, myocardial infarction (MI) transverse aortic constriction (TAC) mouse models were collected determine microtubule associated protein 4 (MAP4)...
SUMMARY Astragaloside IV (AST‐IV) is purified from a natural plant product. Previous studies have shown that AST‐IV has anti‐oxidant activity. In the present study, we investigated effect and mechanism of action on rat cardiomyocytes subjected to hypoxic conditions (up 12 h). Cardiomyocytes were prepared neonatal rats cultured under normoxic or in absence presence (12.5, 25 50 µg/mL). Cell viability, malondialdehyde (MDA) levels, activity expression superoxide dismutase (SOD)‐1 (mRNA protein...
Background Interactions between stromal cell-derived factor-1α (SDF-1α) and its cognate receptor CXCR4 are crucial for the recruitment of mesenchymal stem cells (MSCs) from bone marrow (BM) reservoirs to damaged tissues repair during alarm situations. MicroRNAs differentially expressed in cell niches, suggesting a specialized role regulation. Here, we gain insight into molecular mechanisms involved regulating SDF-1α. Methods MSCs green fluorescent protein transgenic male mice were transfused...
Abnormal lipid metabolism, renal accumulation and lipotoxicity are associated with the pathological features of glomerulopathy. However, mechanisms by which leads to development or progression this disease have not been fully elucidated. In work, we identified a role for rate-limiting enzyme in lipolysis, adipose triglyceride lipase (ATGL; also called patatin-like phospholipase domain-containing protein 2), metabolism kidney disease. ATGL-deficient (Atgl(-/-)) mice displayed albuminuria,...
Both cell migration and proliferation are indispensable parts of reepithelialization during skin wound healing, which is a complex process for the underlying molecular mechanisms largely unknown.Here, we identify novel role microtubule-associated protein 4 (MAP4), cytosolic microtubule-binding that regulates microtubule dynamics through phosphorylation modification, as critical regulator epidermal repair.We showed MAP4 was induced in wounds.In an aberrant phosphorylated mouse model,...
Mitochondrial membrane permeability has received considerable attention recently because of its key role in apoptosis and necrosis induced by physiological events such as hypoxia. The manner which mitochondria interact with other molecules to regulate mitochondrial cell destiny remains elusive. Previously we verified that hypoxia-induced phosphorylation microtubule-associated protein 4 (MAP4) could lead microtubules (MTs) disruption. In this study, established the hypoxic (1% O(2)) models...
The integrity of the vascular barrier, which is essential to blood vessel homoeostasis, can be disrupted by a variety soluble permeability factors during sepsis. Pigment epithelium-derived factor (PEDF), potent endogenous anti-angiogenic molecule, significantly increased in sepsis, but its role endothelial dysfunction has not been defined. To assess PEDF vasculature, we evaluated effects exogenous vivo using mouse model cecal ligation and puncture (CLP)-induced sepsis vitro human dermal...
Endothelial cells play a critical role in vessels the process of angiogenesis during skin wound healing. The migration and proliferation endothelial could be initiated by hypoxic microenvironment wound, while underlying mechanisms remain largely unknown. Here we identify novel for microtubule-associated protein 4 (MAP4) angiogenesis. We firstly demonstrate that MAP4 phosphorylation is induced cells; increase enhances cells. also find hypoxia (2% O2) activates p38/MAPK signaling as an...
The aim of this study was to test the hypothesis that circulating factors released after a severe burn cause endothelial barrier dysfunction by triggering cell (EC) contraction through p38 mitogen-activated protein (MAP) kinase-dependent mechanism. Human umbilical vein ECs (ECV304 line) were cultured create monolayer cells then with 20% human normal or serum. Monolayer permeability measured influx labeled albumin across cells. Endothelial determined alterations surface area and formation...
Abstract Background Diabetic nephropathy (DN) involves various structural and functional changes because of chronic glycemic assault kidney failure. Proteinuria is an early clinical manifestation DN, but the associated pathogenesis remains elusive. This study aimed to investigate role microtubule protein 4 (MAP4) phosphorylation (p-MAP4) in proteinuria DN its possible mechanisms. Methods In this study, urine samples diabetic patients tissues streptozotocin (STZ)-induced mice were obtained...
The aim of this study was to test the hypothesis that circulating factors released after a severe burn cause endothelial barrier dysfunction by triggering cell (EC) contraction through p38 mitogen-activated protein (MAP) kinase-dependent mechanism. Human umbilical vein ECs (ECV304 line) were cultured create monolayer cells then with 20% human normal or serum. Monolayer permeability measured influx labeled albumin across cells. Endothelial determined alterations surface area and formation...
Abstract Phosphorylation of MAP4 (p-MAP4) causes cardiac remodeling, with the microvascular endothelium being considered a vital mediator this process. In current study, we investigated mechanism underlying p-MAP4 influences on density. We firstly confirmed elevated phosphorylation in myocardium knock-in (KI) mice. When compared corresponding control group, detected decreased expression CD31, CD34, VEGFA, VEGFR2, ANG2, and TIE2 KI mice, accompanied by reduced plasma concentration VEGF....