A5063937820- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Amino Acid Enzymes and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- Signaling Pathways in Disease
- RNA Research and Splicing
- Protein Kinase Regulation and GTPase Signaling
- Retinal Development and Disorders
- RNA regulation and disease
- Neurobiology and Insect Physiology Research
- Medicinal Plants and Bioactive Compounds
- Cytokine Signaling Pathways and Interactions
- Multiple Sclerosis Research Studies
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Metabolomics and Mass Spectrometry Studies
- Axon Guidance and Neuronal Signaling
- Viral gastroenteritis research and epidemiology
- Melanoma and MAPK Pathways
- Nerve injury and regeneration
Universidad Autónoma de Madrid
2009-2024
Centro de Biología Molecular Severo Ochoa
2008-2024
Consejo Superior de Investigaciones Científicas
2004-2021
Centro Nacional de Biotecnología
2007-2016
Centro de Estudios Andaluces
2007-2008
Spanish National Centre for Cardiovascular Research
2007-2008
In this study, we present evidence that a glycine transporter, GLYT1, is expressed in neurons and it associated with glutamatergic synapses. Despite the presence of GLYT1 mRNA both glial cells neurons, previous studies have mainly localized immunoreactivity to caudal regions nervous system. However, using novel sequence specific antibodies, identified not only glia, but also neurons. The immunostaining neuronal elements could best be appreciated forebrain areas such as neocortex or...
ABSTRACT African swine fever virus (ASFV) A224L is a member of the inhibitor apoptosis protein (IAP) family. We have investigated antiapoptotic function viral IAP both in stably transfected cells and ASFV-infected cells. was able to substantially inhibit caspase activity cell death induced by treatment with tumor necrosis factor alpha cycloheximide or staurosporine when overexpressed Vero gene transfection. also observed that ASFV infection induces activation Furthermore, using deletion...
Whereas neurons of the lower layers (VI–V) cerebral cortex are first born from dividing precursors at ventricular zone, upper layer (II–IV) subsequently arise divisions intermediate neuronal subventricular zone (SVZ). Little is known about mechanisms that control proliferation SVZ precursors. We herein report restricted expression homeodomain transcription factor Cux-2 in regulates and number neurons. In Cux-2-deficient mice (Cux-2−/−), there excessive selective expansion Double-labeling...
A number of recent reports implicate the differential regulation apical and basal dendrites in autism disorders higher functions human brain. They show that are functionally specialized mechanisms regulating their development have important consequences for neuron function. The molecular identity layer II-III neurons cerebral cortex is determined by overlapping expression Cux1 Cux2. We previously showed both Cux2 necessary nonredundant normal dendrite neurons. Loss function either gene...
Abstract The SNAT5 transporter is a neutral amino acid carrier whose function remains unclear. Structural and mechanistically, closely related to the SNAT3 that mediates efflux of glutamine from glial cells participates in glutamate‐glutamine cycle brain. In this study, we have analyzed distribution rat central nervous system using specific antibodies. Through immunoblotting observed ubiquitously but unevenly distributed CNS. It accumulates most intensely neocortex, hippocampus, striatum,...
Homeodomain transcription factors play important roles in the specification and differentiation of neuronal subpopulations. In cerebral cortex, expression patterns Cux-1 Cux-2 medial ganglionic eminence (MGE) suggest a role for these development interneurons, heterogeneous population. this report, we describe proteins Reelin-secreting interneurons cortical plate, but not calretinin or parvalbumin The Cux genes Reelin positive neurons was studied using knockout mice. These experiments...
Abstract Recent evidence indicates that the glycine transporter‐1 (GLYT1) plays a role in regulation of NMDA receptor function through tight control concentration its surrounding medium. Immunohistochemical studies have demonstrated that, as well being found glial cells, GLYT1 is also associated with pre‐ and postsynaptic aspects glutamatergic synapses. In this article, we describe interaction between PSD‐95 rat brain, scaffolding protein participates organization Mutational analysis reveals...
Rapid and effective neural transmission of information requires correct axonal myelination. Modifications in myelination alter capacity to transmit electric impulses enable pathological conditions. In the CNS, oligodendrocytes (OLs) myelinate axons, a complex process involving various cellular interactions. However, we know little about mechanisms that orchestrate Here, demonstrate OLs express R-Ras1 R-Ras2. Using female male mutant mice delete these proteins, found activation PI3K/Akt...
The glutamate transporter (GLT1) regulates concentrations in glutamatergic synapses and it is expressed at least two isoforms, GLT1a GLT1b. In this work, we show that the C-terminus of GLT1b able to interact with PDZ domains a number proteins. Notably, one them might be scaffold protein post-synaptic density (PSD-95). formed co-immunoprecipitable complexes PSD-95 solubilizated rat brain extracts, also contained NMDA receptors. Co-transfection GLT1b, PSD-95, receptor subunits heterologous...
Abstract Malignant tumors of the central nervous system (CNS) are 10 th most frequent cause cancer mortality. Despite strong malignancy some such tumors, oncogenic mutations rarely found in classic members RAS family small GTPases. This raises question as to whether other may be affected CNS excessively activating pathways. The -related subfamily GTPases is that which closely related classical Ras and it currently contains 3 members: RRAS , RRAS2 RRAS3 . While R-RAS R-RAS2 expressed...
A unique synaptic activity-responsive element (SARE) sequence, composed of the consensus binding sites for SRF, MEF2 and CREB, is necessary control transcriptional upregulation Arc gene in response to activity. We hypothesize that this sequence a broad mechanism regulates expression activation during plasticity; analysis SARE-containing genes could identify molecular mechanisms involved brain disorders. To search conserved SARE sequences mammalian genome, we used SynoR silico tool, found...
The GLYT1 (glycine transporter-1) regulates both glycinergic and glutamatergic neurotransmission by controlling the reuptake of glycine at synapses. Trafficking to cell surface is critical for its function. In present paper, using mutational analysis C-terminal domain, we identified evolutionarily conserved motif R575L576(X8)D585 as being necessary ER (endoplasmic reticulum) export. This probably due capacity bind Sec24D, a component COPII (coatomer coat protein II) complex. export was...
Brain ischemia, also known as ischemic stroke, occurs when there is a lack of blood supply into the brain. When an insult appears, both neurons and glial cells can react in several ways that will determine severity prognosis. This high heterogeneity responses has been major obstacle developing effective treatments or preventive methods for stroke. Although white matter pathophysiology not deeply assessed its remodelling greatly influence clinical outcome disability degree. Oligodendrocytes,...
Dendrites and spines are key regulators of neuronal function often affected in cognitive disorders. Neuronal subclasses characterized by a wide range dendritic morphologies that aid their specific functions. However, how subclass-specific trees arise during vertebrate development remains largely unknown. We have recently reported the restricted expression Cux1 Cux2 genes upper layers cerebral cortex determines morphology dendrites these neurons. Since Cux homologues Drosophila Cut, which...
Fast synaptic transmission in vertebrates is critically dependent on myelin for insulation and metabolic support. Myelin produced by oligodendrocytes (OLs) that maintain multilayered membrane compartments wrap around axonal fibers. Alterations myelination can therefore lead to severe pathologies such as multiple sclerosis. Given hypomyelination disorders have complex etiologies, reproducing clinical symptoms of diseases from a neurological perspective animal models has been difficult. We...
In the mammalian central nervous system, axonal myelination, executed by mature oligodendrocytes (MOLs), enables rapid neural transmission. Conversely, myelin deficiencies are hallmark features of multiple sclerosis, optic neuromyelitis, and some leukodystrophies. Recent studies have highlighted that MOLs heterogeneous; however, how MOL subpopulations specified balanced in physiological settings is poorly understood. Previous works demonstrated an essential role small GTPases R-Ras1 R-Ras2...