Maerl beds are sensitive biogenic habitats built by an accumulation of loose-lying, non-geniculate coralline algae. While these considered hot-spots marine biodiversity, the number and distribution maerl-forming species is uncertain because homoplasy plasticity morphological characters common. As a result, discrimination based on features notoriously challenging, making algae ideal candidates for DNA barcoding study. Here, mitochondrial (COI-5P barcode fragment) plastidial (psbA gene)...

For the first time, morpho-anatomical characters that were congruent with DNA sequence data used to characterize several genera in Hapalidiaceae-the major eco-engineers of Subarctic carbonate ecosystems. sequencing three genes (SSU, rbcL, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit gene and psbA, photosystem II D1 protein gene), along patterns cell division, elongation, calcification supported a monophyletic Clathromorphum. Two diagnostic for this genus: (i) primary...

Lithophyllum species in the Mediterranean Sea function as algal bioconstructors, contributing to formation of biogenic habitats such coralligenous concretions. In habitats, thalli , consisting crusts or lamellae with entire lobed margins, have been variously referred either one species, L. stictiforme two and cabiochiae recent literature. We investigated diversity phylogenetic relationships these algae by sequencing three markers ( psb A rbc L genes, cox 2,3 spacer), conjunction methods for...

DNA sequences from type material in the nongeniculate coralline genus Lithophyllum were used to unambiguously link some European species names field‐collected specimens, thus providing a great advance over morpho‐anatomical identifi‐cation. In particular, sequence comparisons of rbc L, COI and psb A genes specimens allowed following conclusion: generitype species, L. incrustans , occurs mostly as subtidal rhodoliths crusts on both Atlantic Mediterranean coasts, not common, NE Atlantic,...

:Plastid-encoded rbcL and psbA sequences from branched, Caribbean Sea Lithophyllum specimens indicate that four species are present, not one. Short (263 base pairs) an isolectotype of L. kaiseri (Gulf Suez) the holotypes congestum, daedaleum platyphyllum (Caribbean Sea) show congestum conspecific with kaiseri, last having nomenclatural priority. platyphyllum, currently considered a synonym is recognised as valid species. stictaeforme, originally described Mediterranean Sea, (as congestum)...

Background: External volume expansion by suction is used to prepare the recipient site for fat grafting increasing its compliance and vascularity. The authors previously developed a mouse model external demonstrated pro-proliferative angiogenic effects. Increased thickness of subcutaneous tissue was also observed. This study thus designed assess adipogenic potential stimulation. Methods: A miniaturized device consisting rubber dome connected −25 mmHg source applied dorsum mice single 2-hour...

Phymatolithon lusitanicum is a new maerl species described based on an integrative systematic approach including molecular (COI-5P, psbA) and morphological data obtained from recent collections, as well comparison of type material the morphologically ecologically alike NE Atlantic P. lamii laevigatum. Molecular analyses laevigatum were congruent in delimiting independent lineage these crustose species. The three shared common external morphology multiporate asexual conceptacles, but has been...

Although the ecological importance of rhodolith (maerl, free-living coralline algae) beds is well-known, rhodolith-forming species have been neglected in molecular phylogenetic studies. This first systematic study aimed at understanding whether habit a fixed feature lineages and determining relationship (phylogenetic vs. environmental) between crustose habits. Phylogenetic relationships encrusting algae generic levels were analysed using SSU rDNA psbA sequences. Extensive sampling European...

Due to the high plasticity of coralline algae, identification based on morphology alone can be extremely difficult, so studies increasingly use a combination and genetics in species delimitation. A DNA barcoding study was carried out maerl-forming algae using mitochondrial cytochrome oxidase 1 gene, CO1, plastid psbA, field specimens from Falmouth Oban together with herbarium Natural History Museum, UK, Smithsonian Institution, Washington, USA. Results revealed presence north Britain new...

Background: Intermittent external volume expansion using suction enhances the vascular network of soft tissues, possibly increasing fat graft survival. However, optimal kinetics application have not been determined. Based on their previous experience, authors hypothesized that moderate-intensity intermittent may further enhance both angiogenic and adipogenic potential. Methods: Fifty 12-week-old wild-type mice were assigned to five experimental groups ( n = 10 per group) underwent different...

Background: Differentiating between superficial and deep-dermal (DD) burns remains challenging. Superficial-dermal heal with conservative treatment; DD often require excision skin grafting. Decision of surgical treatment is delayed until burn depth definitively identified. This study's aim to assess the ability hyperspectral imaging (HSI) differentiate depth. Methods: Thermal injury graded severity was generated on dorsum hairless mice a heated brass rod. Perfusion oxygenation parameters...

Background: Necrosis remains a significant complication in cutaneous flap procedures. Monitoring, and ideally prediction, of vascular compromise the early postoperative period may allow surgeons to limit impact complications by prompt intervention. Hyperspectral imaging could be reliable, effective, noninvasive method for predicting survival postoperatively. In this preclinical study, authors demonstrate that hyperspectral is able correlate skin perfusion changes ultimate model. Methods:...

Fertile gametangial plants of Phymatolithon calcareum, which are seldom reported in the Atlantic European coasts, were collected as encrusting, epilithic a subtidal maerl bed Brittany (France). Based on their morphological features, identified P. calcareum. This identification was further confirmed by DNA barcodes using reference COI-5P sequences obtained from neotype together with recent collections beds. The reproductive structures empty but they regarded mature female conceptacles....

Lithophyllum byssoides is a common coralline alga in the intertidal zone of Mediterranean coasts, where it produces biogenic concretions housing high algal and invertebrate biodiversity. This species an ecosystem engineer considered target for conservation efforts, but designing effective strategies currently impossible due to lack information about its population structure. The morphological molecular variation L. was investigated using morphoanatomy DNA sequences ( psb A cox 2,3) obtained...

The Abrolhos Continental Shelf (ACS) encompasses the largest and richest coral reefs in southern Atlantic Ocean. A taxonomic study of non-geniculate coralline algae (NGCA) from region was undertaken using both morpho-anatomical molecular data. Specimens NGCA were collected 2012 2014 shallow ACS. Phylogenetic analysis performed dataset psbA DNA sequences 16 specimens ACS additional GenBank related species. Nine common tropical reef-building species identified described: Hydrolithon...

Background: External volume expansion prepares recipient sites to improve outcomes of fat grafting. For patients receiving radiotherapy after mastectomy, results with external vary, and the relationship between remains unexplored. Thus, authors developed a new translational model investigate effects in chronic skin fibrosis radiation exposure. Methods: Twenty-four SKH1-E mice received 50 Gy β-radiation each flank were monitored until (8 weeks). was then applied at −25 mmHg one side for 6...

Adipose tissue (AT) is used extensively in reconstructive and regenerative therapies, but transplanted fat often undergoes cell death, leading to inflammation, calcification, requirement for further revision surgery. Previously, we have found that mesenchymal progenitor cells within human AT can proliferate three-dimensional culture under proangiogenic conditions. These (primed ADipose cells, PADS) robustly differentiate into adipocytes vitro (ad-PADS). The goal of this study determine...

Velopharyngeal insufficiency (VPI) results from incomplete closure of the velopharyngeal (VP) sphincter with oral pressure consonants during speech. Maxillary hypoplasia is common among cleft children and often requires LeFort I advancement. This in anterior movement soft palate bony maxillary segment. Consequently, size VP increased may result postoperative VPI or worsening prior VPI. To better counsel our patients their families risk after advancement, we chose to evaluate own cohort.We...

Plastic surgery continues to be one of the most competitive programs into which medical students attempt match. The reasons for this include complexity and diversity cases, opportunity interact with multiple specialties, patient surgeon satisfaction, potential higher compensation compared other specialties. disparity between relatively small number plastic residency positions large highly candidates has created challenges both applicant program director. This challenge is even greater...

Several species in the red algal genus Halymenia from Indo-Pacific have been described with branched thalli, toothed margins, spinose proliferations on blade, and a firm gelatinous texture. Previous works synonymized many of these morphologically similar H. durvillei. Our increased taxon sampling molecular data indicate that taxonomy is need revision, several aspects taxonomies proposed by previous authors now seem unlikely. Thus, aim present work was to analyse delimitation species....

A constant effort in sequencing an extensive number of specimens originating from comprehensive sampling had return unprecedented amount information fostering our understanding diversity, evolution and distribution coralline algae; however, many sequences lack reliable assignation a taxonomic name, specially at the species level. Recently, type material allowed to bridge this gap by providing direct link between DNA sequence bearing name. For instance, genus Lithophyllum, identity three...