Abstract Photosynthetic microbes are of emerging interest as production organisms in biotechnology because they can grow autotrophically using sunlight, an abundant energy source and CO 2 , a greenhouse gas. Important traits for such fast growth amenability to genetic manipulation. Here we describe Synechococcus elongatus UTEX 2973, unicellular cyanobacterium capable rapid autotrophic growth, comparable heterotrophic industrial hosts yeast. 2973 be readily transformed facile generation...

The development and utilization of collision reaction cells in atomic mass spectrometry is reviewed. These devices have been used for decades fundamental studies ion–molecule chemistry only recently applied the GD-MS ICP-MS fields. Such are to promote reactive non-reactive collisions, with resultant benefits interference reduction, isobar separation, thermalization/focusing ions ICP-MS. Novel schemes, using a variety gas reagents selected on basis thermodynamic kinetic principles data, now...

We provide the initial performance evaluation of a 21 Tesla Fourier transform ion cyclotron resonance mass spectrometer operating at Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory. The constructed for 21T system employs commercial dual linear trap coupled to FTICR designed and built in-house. Performance gains from moving higher magnetic field strength are exemplified by measurement peptide isotopic fine structure, complex natural organic matter mixtures,...

Reversible protein thiol oxidation is an essential regulatory mechanism of photosynthesis, metabolism, and gene expression in photosynthetic organisms. Herein, we present proteome-wide quantitative site-specific profiling vivo modulated by light/dark the cyanobacterium Synechocystis sp. PCC 6803, oxygenic prokaryote, using a resin-assisted enrichment approach. Our proteomic approach integrates with isobaric tandem mass tag labeling to enable measurements reversibly oxidized thiols. The redox...

Cyanobacteria, blue-green algae, are the most abundant autotrophs in aquatic environments and form base of food chain by fixing carbon nitrogen into cellular biomass. To compensate for low selectivity Rubisco CO2 over O2, cyanobacteria have developed highly efficient CO2-concentrating machinery which ABC transport system CmpABCD from Synechocystis PCC 6803 is one component. Here, we described structure bicarbonate-binding protein CmpA absence presence bicarbonate carbonic acid. homologous to...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInductively coupled helium plasma as an ion source for mass spectrometryAkbar. Montaser, Shi Kit. Chan, and David W. KoppenaalCite this: Anal. Chem. 1987, 59, 8, 1240–1242Publication Date (Print):April 15, 1987Publication History Published online1 May 2002Published inissue 15 April 1987https://pubs.acs.org/doi/10.1021/ac00135a038https://doi.org/10.1021/ac00135a038research-articleACS PublicationsRequest reuse permissionsArticle...

In this study, the three-dimensional spatial distributions of a number metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many exhibited distinct compartmentalization, some asymmetrically distributed throughout nodule (e.g., S-adenosylmethionine). These results establish more complex metabolic view plant-bacteria (and BNF)...

Technologies enabling in situ metabolic profiling of living plant systems are invaluable for understanding physiological processes and could be used rapid phenotypic screening (e.g., to produce plants with superior biological nitrogen-fixing ability). The symbiotic interaction between legumes soil bacteria results a specialized organ (i.e., root nodule) where the exchange nutrients host endosymbiont occurs. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is method that...

Mass spectrometry (MS) is an indispensable analytical tool to capture the array of metabolites within complex biological systems. However, conventional MS-based metabolomic workflows require extensive sample processing and separation resulting in limited throughput potential alteration native molecular states these Ambient ionization methods, capable sampling directly from tissues, circumvent some issues but high-performance MS resolve complexity samples. Here, we demonstrate a unique...

A plasma source ion trap (PSIT) mass spectrometer has been modified to incorporate a radiofrequency octopole guide/collision cell between the and spectrometer. This modification allows ions sampled from undergo reactions prior spectrometric analysis. capability can obviate need for chemical or chromatographic separation of sample, remove spectral interferences enable formation analytically useful molecular ions. Distinct reaction chemistries be utilized in and/or trap. Performance instrument...

We report a new method for selective removal of argon ions and other plasma matrix in source MS. The consists sampling the reacting sampled analyte with hydrogen gas. Reactions have been studied three instruments: ion trap (PSIT) mass spectrometer post-skimmer region both conventional ICP second PSIT. In trap, reaction between Ar+ H2 proceeds at nearly collisional rate whereas most atomic is four to five orders magnitude slower. For modest pressures times [10–4 Torr (1 = 133.322 Pa) 10 ms],...

ADVERTISEMENT RETURN TO ISSUEPREVFeaturesNEXTMS DetectorsDavid W. Koppenaal, Charles J. Barinaga, M. Bonner Denton, Roger P. Sperline, Gary Hieftje, Gregory D. Schilling, Francisco Andrade, James H. Barnes, and IV IVCite this: Anal. Chem. 2005, 77, 21, 418 A–427 APublication Date (Web):November 1, 2005Publication History Published online1 November 2005Published inissue 1 2005https://pubs.acs.org/doi/10.1021/ac053495phttps://doi.org/10.1021/ac053495pnewsACS Publications. This publication is...

Cyanothece sp. ATCC 51142 is a diazotrophic cyanobacterium notable for its ability to perform oxygenic photosynthesis and dinitrogen fixation in the same single cell. Previous transcriptional analysis revealed that existence of these incompatible cellular processes largely depends on tightly synchronized expression programs involving ∼30% genes genome. To expand upon current knowledge, we have utilized sensitive proteomic approaches examine impact diurnal rhythms protein complement 51142. We...

In order to meet a growing need for fieldable mass spectrometer systems precise elemental and isotopic analyses, the liquid sampling-atmospheric pressure glow discharge (LS-APGD) has number of very promising characteristics. One key set attributes that await validation deals with performance characteristics relative isotope ratio precision accuracy. Owing its availability prior experience this research team, initial evaluation (IR) was performed on Thermo Scientific Exactive Orbitrap...

In mass spectrometry, several advantages can be derived when multiple mass-to-charge values are detected simultaneously and continuously. One such advantage is an improved duty cycle, which leads to superior limits of detection, better precision, shorter analysis times, reduced sample sizes. A second the ability reduce correlated noise by taking ratio two or more collected signals, enabling greatly enhanced isotope data. final elimination spectral skew, leading accurate transient signal...

Abstract Background Unicellular cyanobacteria of the genus Cyanothece are recognized for their ability to execute nitrogen (N 2 )-fixation in dark and photosynthesis light. An understanding these mechanistic processes an integrated systems context should provide insights into how might be optimized specialized environments and/or industrial purposes. Systems-wide dynamic proteomic profiling with mass spectrometry (MS) analysis reveal fundamental control regulation functions. Results To...

Cyanobacteria are photosynthetic microbes with highly differentiated membrane systems. These organisms contain an outer membrane, plasma and internal system of thylakoid membranes where the respiratory machinery found. This existence compartmentalization differentiation systems poses a number challenges for cyanobacterial cells in terms organization distribution proteins to correct system. Proteomics studies have long sought identify components different cyanobacteria, date about 450 been...

The coupling of an LS-APGD microplasma with Orbitrap analyzer is applied to the isotope ratio analysis uranium.

A Mattauch-Herzog geometry mass spectrograph (MHMS) has been equipped with a novel array detector, the focal plane camera (FPC). The FPC consists of an gold Faraday cups, each coupled to its own integrator, interrogation integrators performed by multiplexer. initial coupling this instrument pin-type glow discharge source provided limits detection in single hundreds nanograms per gram regime; isotope ratio accuracy and precision better than 5% error 0.2% RSD, respectively; linear dynamic...