A5055434986- Neurobiology and Insect Physiology Research
- Cancer therapeutics and mechanisms
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Animal Behavior and Reproduction
- Cellular transport and secretion
- DNA Repair Mechanisms
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Invertebrate Immune Response Mechanisms
- Genomics and Chromatin Dynamics
- Bioactive Compounds and Antitumor Agents
- Toxin Mechanisms and Immunotoxins
- PI3K/AKT/mTOR signaling in cancer
- Photoreceptor and optogenetics research
- Axon Guidance and Neuronal Signaling
- Chromosomal and Genetic Variations
- Synthesis and Biological Evaluation
- Neuroscience and Neuropharmacology Research
- Insect and Arachnid Ecology and Behavior
- Wnt/β-catenin signaling in development and cancer
- Olfactory and Sensory Function Studies
- Bacillus and Francisella bacterial research
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2012-2024
National Institutes of Health
2012-2024
Health and Human Development (2HD) Research Network
2018-2019
Eunice Kennedy Shriver Center
2014
TE Connectivity (Switzerland)
2014
Institute of Molecular Biology, Academia Sinica
2000-2006
National Defense University
2005
National Yang Ming Chiao Tung University
2003
Academia Sinica
1997-2002
DNA topoisomerase II (TOP2) cleavable complexes represent an unusual type of damage characterized by reversible TOP2-DNA cross-links and double strand breaks. Many antitumor drugs physiological stresses are known to induce TOP2 leading apoptotic cell death genomic instability. However, the molecular mechanism(s) for repair remains unclear. In current studies, we show that induced prototypic poison VM-26 proteolytically degraded ubiquitin/26 S proteasome pathway. Surprisingly TOP2β isozyme is...
Color and motion information are thought to be channeled through separate neural pathways, but it remains unclear whether how these pathways interact improve perception. In insects, such as Drosophila, has long been believed that is fed exclusively by one spectral class of photoreceptor, so-called R1 R6 cells; whereas R7 R8 photoreceptors, which exist in multiple classes, subserve color vision. Here, we report also contribute the pathway. By using electrophysiological, optical, behavioral...
The receptor mechanism for color vision has been extensively studied. In contrast, the circuit(s) that transform(s) photoreceptor signals into percepts to guide behavior remain(s) poorly characterized. Using intersectional genetics inactivate identified subsets of neurons, we have uncovered first-order interneurons are functionally required hue discrimination in Drosophila. We developed a novel aversive operant conditioning assay intensity-independent (true vision) Single flying flies...
Visual information received from the three types of photoreceptor neurons(R1-R6, R7 and R8) in fly compound eyes converges to external part medulla neuropil (M1-M6 layers) a layer-specific fashion: R8 axons terminate at M6 M3 layers, respectively, whereas lamina neurons(L1-L5) relay R1-R6 multiple layers (M1-M5). Here, we show that during development, neurons establish projections two separate stages: first stage, sequentially target R7- R8-temporary respectively; second growth cones...
Here we report the development of a ternary version LexA::VP16/LexAop system in which DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA can be concatenated with Gal4/upstream activating sequence refine patterns existing Gal4 lines minimal genetic manipulations.
ABSTRACT In Drosophila , color vision and wavelength‐selective behaviors are mediated by the compound eye's narrow‐spectrum photoreceptors R7 R8 their downstream medulla projection (Tm) neurons Tm5a, Tm5b, Tm5c, Tm20 in second optic neuropil or medulla. These chromatic Tm project axons to a deeper neuropil, lobula, which insects has been implicated processing relaying information central brain. The synaptic targets of lobula not known, however. Using modified GFP reconstitution across...
It has been proposed that the topoisomerase II (TOP2)β–DNA covalent complex arrests transcription and triggers 26S proteasome-mediated degradation of TOP2β. is unclear whether initial trigger for proteasomal due to DNA damage or transcriptional arrest. In current study we show TOP2 catalytic inhibitor 4,4-(2,3-butanediyl)-bis(2,6-piperazinedione) (ICRF-193), which traps into a circular clamp rather than TOP2–DNA complex, can also arrest transcription. Arrest transcription, TOP2β-dependent,...
Human (h) DNA topoisomerase I has been identified as a major SUMO1 target in camptothecin-treated cells. In response to TOP1-mediated damage induced by camptothecin, multiple molecules are conjugated the N-terminal domain of single TOP1 molecule. To investigate molecular mechanism conjugation TOP1, an vitro system using purified SAE1/2, Ubc9, SUMO1, and peptides was developed. Consistent with results from vivo studies, were found be Systematic analysis site located between amino acid...
Establishing appropriate sizes and shapes of dendritic arbors is critical for proper wiring the central nervous system. Here we report that Insulin-like Peptide 2 (DILP2) locally activates transiently expressed insulin receptors in dendrites Drosophila Dm8 amacrine neurons to positively regulate field elaboration. We found DILP2 was L5 lamina neurons, which have axonal terminals abutting dendrites. Proper dendrite morphogenesis synapse formation required signaling through TOR (target...
The TORC1 regulator GATOR1/SEACIT controls meiotic entry and early events in yeast. However, how metabolic pathways influence progression metazoans remains poorly understood. Here we examine the role of regulators GATOR1 GATOR2 response to double-stranded breaks (DSB) during