A5063716094- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Amyotrophic Lateral Sclerosis Research
- PI3K/AKT/mTOR signaling in cancer
- CRISPR and Genetic Engineering
- Mast cells and histamine
- Cannabis and Cannabinoid Research
- Blood Pressure and Hypertension Studies
- Osteoarthritis Treatment and Mechanisms
- Neuroscience and Neuropharmacology Research
- Studies on Chitinases and Chitosanases
- Heart Rate Variability and Autonomic Control
- RNA regulation and disease
- Parkinson's Disease Mechanisms and Treatments
- Microtubule and mitosis dynamics
- Biofuel production and bioconversion
- Dendrimers and Hyperbranched Polymers
- Bone Tumor Diagnosis and Treatments
- Statistical Methods and Inference
- Plant Gene Expression Analysis
- Bone and Joint Diseases
- Neurogenesis and neuroplasticity mechanisms
- Polyamine Metabolism and Applications
North Carolina State University
2024
Macquarie University
2021-2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2020-2024
National Institutes of Health
2020-2024
Hunan Provincial People's Hospital
2024
Hunan Normal University
2024
National Center for Advancing Translational Sciences
2022
Nanjing Foreign Language School
2021
Georgetown University
2014-2017
Soochow University
2017
Macroautophagy/autophagy is a cellular degradation process that sequesters organelles or proteins into double-membrane structure called the phagophore; this transient compartment matures an autophagosome, which then fuses with lysosome vacuole to allow hydrolysis of cargo. Factors control membrane traffic are also essential for each step autophagy. Here we demonstrate 2 monomeric GTP-binding in Saccharomyces cerevisiae, Arl1 and Ypt6, belong Arf/Arl/Sar protein family Rab family,...
Amyotrophic Lateral Sclerosis (ALS) is a severe neurodegenerative disease affecting motor neurons. Pathological forms of Tar-DNA binding protein-43 (TDP-43), involving its mislocalisation to the cytoplasm and formation misfolded inclusions, are present in almost all ALS cases (97%), ~ 50% related condition, frontotemporal dementia (FTD), highlighting importance neurodegeneration. Previous studies have shown that endoplasmic reticulum protein 57 (ERp57), member disulphide isomerase (PDI)...
Ypt/Rab are key regulators of intracellular trafficking in all eukaryotic cells. In yeast, Ypt1 is essential for endoplasmic reticulum (ER)-to-Golgi transport, whereas Ypt31/32 regulate Golgi-to-plasma membrane and endosome-to-Golgi transport. TRAPP a multisubunit complex that acts as an activator GTPases. Trs85 Trs130 two subunits specific III II, respectively. Whereas was shown to activator, it still controversial whether II or activator. Here, we use GFP-Snc1 tool study transport Ypt...
Abstract Treatment for anterior cruciate ligament (ACL) tears depends on the condition of ligament. We aimed to identify different tear statuses from preoperative MRI using deep learning-based radiomics with sex and age. reviewed 862 patients scans reflecting ACL status Hunan Provincial People’s Hospital. Based sagittal proton density-weighted images, a fully automated approach was developed that consisted learning model segmenting tissue (ACL-DNet) recognizer classification (ACL-SNet). The...
Target of Rapamycin Complex I (TORC1) is a central regulator metabolism in eukaryotes that responds to wide array negative and positive inputs. The GTPase-activating protein toward Rags (GATOR) signaling pathway acts upstream TORC1 comprised two subcomplexes. trimeric GATOR1 complex inhibits activity response amino acid limitation by serving as (GAP) for the activator RagA/B, component lysosomally located Rag GTPase. multi-protein GATOR2 thus promotes activation. Here we report Wdr59,...
Abstract In Saccharomyces cerevisiae, Arl1 and Ypt6, two small GTP-binding proteins that regulate membrane traffic in the secretory endocytic pathways, are also necessary for autophagy. To gain information about potential partners of Ypt6 specifically autophagy, we carried out a high copy number suppressor screen to identify genes when overexpressed suppress rapamycin sensitivity phenotype arl1Δ ypt6Δ strains at 37°. From results, selected COG4, SNX4, TAX4, IVY1, PEP3, SLT2, ATG5, either or...
When evaluating the effectiveness of a drug, Randomized Controlled Trial (RCT) is often considered gold standard due to its perfect randomization. While RCT assures strong internal validity, restricted external validity poses challenges in extending treatment effects broader real-world population possible heterogeneity covariates. In this study, we employed augmented inverse probability sampling weighted (AIPSW) estimator generalize findings from an comparing efficacy Songling Xuemaikang...
To estimate the average treatment effect in real-world populations, observational studies are typically designed around cohorts. However, even when study samples from these designs represent population, unmeasured confounders can introduce bias. Sensitivity analysis is often used to bounds for without relying on strict mathematical assumptions of other existing methods. This article introduces a new approach that improves sensitivity by incorporating randomized clinical trial data, with...
Abstract Background Mutations in the CCNF gene encoding cyclin F are associated with sporadic and familial amyotrophic lateral sclerosis (ALS) frontotemporal dementia, but underlying pathophysiological mechanisms unknown. Proper functioning of endoplasmic reticulum (ER) is essential for physiological cellular function. Methods We used human neuroblastoma SH-SY5Y embryonic kidney HEK293T cell lines mouse primary neurons-overexpressing two ALS mutants to examine whether mutant...
Neurons originate from stem cells. Adult neurogenesis continuously occurs in specific areas of the rostral lateral ventricles and hippocampus. Microglia is an essential components normal hippocampus as it can remove kill damaged cells infections through phagocytosis to help hippocampal neurons stay homeostasis. Internal external stimuli trigger different responses microglia. Inhibition neuron firing would disrupt function like synapse formation synaptic function, which increase microglia...