A5078913881- Fungal and yeast genetics research
- Cellular transport and secretion
- Mycotoxins in Agriculture and Food
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Ion Transport and Channel Regulation
- Plant Parasitism and Resistance
- Lysosomal Storage Disorders Research
- Plant Stress Responses and Tolerance
- Plant Pathogenic Bacteria Studies
- Fermentation and Sensory Analysis
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Toxoplasma gondii Research Studies
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Biochemical and biochemical processes
- Lignin and Wood Chemistry
- Insect and Pesticide Research
- Polyamine Metabolism and Applications
Shandong Agricultural University
2018-2025
State Key Laboratory of Crop Biology
2020-2021
Nanjing Agricultural University
2012-2017
Hangzhou Dianzi University
2016
Modification of target molecules by ubiquitin or ubiquitin-like (Ubl) proteins is generally reversible. Little known, however, about the physiological function reverse reaction, deconjugation. Atg8 a unique Ubl protein whose conjugation lipid phosphatidylethanolamine (PE). functions in formation double-membrane autophagosomes, central step well-conserved intracellular degradation pathway macroautophagy (hereafter autophagy). Here we show that deconjugation Atg8−PE cysteine protease Atg4...
The plant signaling pathway that regulates pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) involves mitogen-activated protein kinase (MAPK) cascades comprise sequential activation of several kinases and the ensuing phosphorylation MAPKs, which activate transcription factors (TFs) to promote downstream defense responses. To identify TFs regulate we investigated TF-defective mutants Arabidopsis thaliana identified MYB44 as an essential constituent PTI pathway. confers...
In autophagy, the double-membrane autophagosome delivers cellular components for their degradation in lysosome. The conserved Ypt/Rab GTPases regulate all trafficking pathways, including autophagy. These function modules that include guanine-nucleotide exchange factor (GEF) activators and downstream effectors. Rab7 its yeast homologue, Ypt7, context of such a module, fusion both late endosomes autophagosomes with yeast, Rab5-related Vps21 is known role early- to late-endosome transport. Here...
In the conserved autophagy pathway, double-membrane autophagosome (AP) engulfs cellular components to be delivered for degradation in lysosome. While only sealed AP can productively fuse with lysosome, molecular mechanism of closure is currently unknown. Rab GTPases, which regulate all intracellular trafficking pathways eukaryotes, also autophagy. Rabs function GTPase modules together their activators and downstream effectors. yeast, an autophagy-specific Ypt1 module, a set autophagy-related...
Fusarium graminearum is recognized as the pathogen responsible for wheat head blight. It produces deoxynivalenol (DON) during infection, which endangers human health. DON biosynthesis occurs within toxisomes in endoplasmic reticulum (ER). In eukaryotes, ER membrane protein complex (EMC) critical ER's normal operation. However, specific role of EMC F. remains poorly understood. this study, six subunits (FgEmc1-6) were identified graminearum, and all them localized to toxisomes. Our results...
Trs130 is a specific component of the transport protein particle II complex, which functions as guanine nucleotide exchange factor (GEF) for Rab GTPases Ypt31/32. Ypt31/32 known to be involved in autophagy, although precise mechanism has not been thoroughly studied. In this study, we investigated potential involvement autophagy and found that both cytoplasm-to-vacuole targeting (Cvt) pathway starvation-induced were defective trs130ts (trs130 temperature-sensitive) mutant. Mutant cells could...
Ypt/Rab are key regulators of intracellular trafficking in all eukaryotic cells. In yeast, Ypt1 is essential for endoplasmic reticulum (ER)-to-Golgi transport, whereas Ypt31/32 regulate Golgi-to-plasma membrane and endosome-to-Golgi transport. TRAPP a multisubunit complex that acts as an activator GTPases. Trs85 Trs130 two subunits specific III II, respectively. Whereas was shown to activator, it still controversial whether II or activator. Here, we use GFP-Snc1 tool study transport Ypt...
Fusarium graminearum is one of the causal agents head blight and produces trichothecene mycotoxin, deoxynivalenol (DON). Thioredoxin reductases (TRRs) play critical roles in recycling oxidized thioredoxin. However, their functions are not well known plant pathogenic fungi. In this study, we characterized a TRR orthologue FgTRR F. graminearum. The FgTRR-GFP fusion protein localized to cytoplasm. gene deletion demonstrated that involved hyphal growth, conidiation, sexual reproduction, DON...
Fusarium graminearum, the main pathogenic fungus causing head blight (FHB), produces deoxynivalenol (DON), a key virulence factor, which is synthesized in endoplasmic reticulum (ER). Sey1/atlastin, dynamin-like GTPase protein, known to be required for homotypic fusion of ER membranes, but functions this protein are unknown fungi. Here, we characterized Sey1/atlastin homologue FgSey1 F. graminearum Like located ER. The FgSEY1 deletion mutant exhibited significantly reduced vegetative growth,...
Plant aquaporins are a recently noted biological resource with great potential to improve crop growth and defense traits. Here, we report the functional modulation of rice (Oryza sativa) aquaporin OsPIP1;3 enhance photosynthesis grain production control bacterial blight leaf streak, most devastating worldwide diseases in crop. We characterize as physiologically relevant CO2 -transporting facilitator, which supports 30% on average. This role is nullified by interaction protein Hpa1, an...
Plants employ aquaporins (AQPs) of the plasma membrane intrinsic protein (PIP) family to import environmental substrates, thereby affecting various processes, such as cellular responses regulated by signaling molecule hydrogen peroxide (H2O2). Common wheat (Triticum aestivum) contains 24 candidate members PIP family, designated TaPIP1;1 TaPIP1;12 and TaPIP2;1 TaPIP2;12. None these TaPIP candidates have been characterized for substrate selectivity or defense in their source plant. Here, we...
Autophagy is a degradation pathway in eukaryotic cells which aging proteins and organelles are sequestered into double-membrane vesicles, termed autophagosomes, fuse with vacuoles to hydrolyze cargo.The key step autophagy the formation of requires different kinds including COPII vesicles Atg9containing transport lipid double-membranes phagophore assembly site (PAS).In yeast, cis-Golgi localized t-SNARE protein Sed5 plays role endoplasmic reticulum (ER)-Golgi intra-Golgi vesicular...
Ypt/Rab GTPases coordinately regulate vesicle trafficking in yeasts. Previously, Ypt1 was shown to suppress growth defects of Ypt6 and its related mutants (ypt6ts, ric1∆, rgp1∆, ric1∆rgp1∆), but the physiological role this suppression has not been well studied. We have investigated effects on two major pathways, autophagy, mutants. restores Snc1 transport plasma membrane via Golgi exocytic pathway under nutrient rich conditions. Overexpression suppresses autophagic starvation conditions with...
Golgins are coiled-coil proteins that play prominent roles in maintaining the structure and function of Golgi complex. However, role golgin phytopathogenic fungi remains poorly understood. In this study, we functionally characterized Fusarium graminearum protein RUD3, a homolog ScRUD3/GMAP-210 Saccharomyces cerevisiae mammalian cells. Cellular localization observation revealed RUD3 is located cis-Golgi. Deletion caused defects vegetative growth, ascospore discharge, deoxynivalenol (DON)...
Abstract The environmentally friendly antibiotic phenazine-1-carboxylic acid (PCA) protects plants, mammals and humans effectively against various fungal pathogens. However, the mechanism by which PCA inhibits or kills pathogens is not fully understood. We analyzed effects of on growth two model organisms, Saccharomyces cerevisiae Candida albicans , found that inhibited yeast in a dose-dependent manner was inversely dependent pH. In contrast, commonly used hygromycin B acted as pH increased....
Fusarium graminearum is a plant filamentous pathogenic fungi and the predominant causal agent of head blight (FHB) in cereals worldwide. The regulators secretory pathway contribute significantly to fungal mycotoxin synthesis, development, virulence. However, their roles these processes F. remain poorly understood. Here, we identified functionally characterized endoplasmic reticulum (ER) cargo receptor FgErv14 graminearum. Firstly, it was observed that mainly localized ER. Then, constructed...
Mitochondrial porin, the voltage-dependent anion-selective channel (VDAC), is most abundant protein in outer membrane, and critical for exchange of metabolites phospholipids yeast mammals. However, functions porin phytopathogenic fungi are not known. In this study, we characterized a orthologue, Fgporin, Fusarium graminearum. The deletion Fgporin resulted defects hyphal growth, conidiation, perithecia development. mutant showed reduced virulence, deoxynivalenol production, lipid droplet...