A5101687900- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- Neuroblastoma Research and Treatments
- Histone Deacetylase Inhibitors Research
- Bladder and Urothelial Cancer Treatments
- Acute Lymphoblastic Leukemia research
- Protein Degradation and Inhibitors
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Renal and related cancers
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- PARP inhibition in cancer therapy
- Telomeres, Telomerase, and Senescence
- Cancer, Hypoxia, and Metabolism
- Machine Learning in Bioinformatics
Northeast Forestry University
2022-2025
Mianyang Normal University
2024
Kunming Medical University
2023-2024
Guiyang Medical University
2019-2024
Affiliated Hospital of Guizhou Medical University
2019-2024
Institute of Zoology
2024
Chinese Academy of Sciences
2024
State Key Laboratory of Stem Cell and Reproductive Biology
2024
University of Chinese Academy of Sciences
2024
Yan'an University
2024
The N-Myc oncoprotein is a critical factor in neuroblastoma tumorigenesis which requires additional mechanisms converting low-level to high-level expression. protein stabilized when phosphorylated at Serine 62 by ERK protein. Here we describe novel positive feedback loop whereby directly induced the transcription of class III histone deacetylase SIRT1, turn increased stability. SIRT1 binds Myc Box I domain form transcriptional repressor complex gene promoter mitogen-activated kinase...
Patients with neuroblastoma due to N-Myc oncogene amplification have a high frequency of tumor metastasis. However, it is not clear how induces cell migration, invasion and The histone demethylase JMJD1A activates gene transcription by demethylating the lysine 9 residue H3 (H3K9) at target promoters. long noncoding RNA MALAT1 lung cancer migration plays pivotal role in Here we demonstrated that up-regulated expression oncogene-amplified human cells directly binding promoter. Affymetrix...
Clinical trials of CD19-specific chimeric antigen receptor (CAR19) T cells have demonstrated remarkable efficacy against relapsed and refractory B cell malignancies. The piggyBac transposon system offers a less complex more economical means for generating CAR19 compared to viral vectors. We previously optimized protocol the generation using system, but we found that had poor in vivo persistence, probably due deleterious FcγR interactions with CAR's IgG1 Fc-containing spacer domain. therefore...
Saline-alkali stress is one of the main abiotic stresses that constrains plant growth. Understanding response mechanism ornamental plants to saline-alkali great significance for improving landscape greening. Chrysanthemum a good with strong resistance stress, rich colors and easy management. Using TMT quantitative proteomics technology, leave root were either untreated or treated 200 mM NaCl 12 h, screened differentially expressed proteins. The results showed 66 452 differential proteins...
TERT gene rearrangement with transcriptional superenhancers leads to overexpression and neuroblastoma. No targeted therapy is available for clinical trials in patients TERT-rearranged neuroblastoma.Anticancer agents exerting the best synergistic anticancer effects BET bromodomain inhibitors were identified by screening an FDA-approved oncology drug library. The of inhibitor OTX015 proteasome carfilzomib examined immunoblot flow cytometry analysis. efficacy combination was investigated mice...
Protein solubility plays a crucial role in various biotechnological, industrial, and biomedical applications. With the reduction sequencing gene synthesis costs, adoption of high-throughput experimental screening coupled with tailored bioinformatic prediction has witnessed rapidly growing trend for development novel functional enzymes interest (EOI). High protein rates are essential this process accurate is challenging task. As deep learning technology continues to evolve, attention-based...
Small-molecule inhibitors of glycogen synthase kinase 3β (GSK3β) have demonstrated strong anti-leukemia effects in preclinical studies. Here, we investigated the effect GSK3β inhibitor 6-Bromoindirubin-3-oxime (BIO) previously shown to inhibit leukemia cell growth vitro and animal models on hematopoietic regeneration recipients stem transplant. BIO administered immunocompromised mice transplanted with human cells inhibited engraftment bone marrow (BM) peripheral blood. reduced CD34(+)...
Abstract Objectives Umbilical cord blood ( UCB ) is a source of stem cells used for allogeneic transplantation, in addition to bone marrow and peripheral blood. Limited numbers single unit associated with slow haematopoietic recovery high risk graft failure, particularly adult patients. can be expanded ex vivo ; however, rapid differentiation reduces their regenerative potential. We have recently shown that Wnt/β‐catenin signalling down‐regulated ‐expanded cells; therefore, we propose...
Despite the success of chimeric antigen receptor (CAR) T cells in clinical studies, a significant proportion responding patients eventually relapsed, with latter correlating low CAR cell expansion and persistence.Using patient-derived xenograft (PDX) mouse models CD19+ B acute lymphoblastic leukemia (B-ALL), we show that priming leukemia-bearing mice 5-azacytidine (AZA) enhances therapy. AZA given 1 day prior to infusion delayed growth promoted effector function. Priming increased...
Osteoarthritis (OA) is a highly prevalent age-related musculoskeletal disorder that typically results in chronic pain and disability. OA multifactorial disease, with increased oxidative stress, dysregulated inflammatory response, impaired matrix metabolism contributing to its onset progression. The neurohormone melatonin, primarily synthesized by the pineal gland, has emerged as promising therapeutic agent for due potential alleviate inflammation, chondrocyte death minimal adverse effects....
Abstract Protein solubility plays a crucial role in various biotechnological, industrial and biomedical applications. With the reduction sequencing gene synthesis costs, adoption of high-throughput experimental screening coupled with tailored bioinformatic prediction has witnessed rapidly growing trend for development novel functional enzymes interest (EOI). High protein rates are essential this process accurate is challenging task. As deep learning technology continues to evolve,...
Ecological stoichiometry is essential for investigating biogeochemical cycling in an ecosystem. Thinning, a management practice that closely mimics natural processes, significantly influences stand structure and microclimate, thereby affecting nutrient cycling. Nonetheless, seasonal variations ecological across the leaf-litter-soil continuum under different thinning regimes remain inadequately understood. In this study, we evaluated three methods (strip filling (SF), (ET), forest gap (FG))...
Chimeric antigen receptor (CAR) T cells targeting CD19 have demonstrated remarkable efficacy in the treatment of B cell malignancies. Current CAR manufacturing protocols are complex and costly due to their reliance on viral vectors. Non-viral systems genetic modification, such as with transposase transposon systems, offer a potential streamlined alternative for manufacture currently being evaluated clinical trials. In this study, we utilized previously described from little brown bat,...
Objective: To study the incidence of bloodstream infections, pathogen distribution, and antibiotic resistance profile in patients with hematological malignancies. Methods: From January 2018 to December 2021, we retrospectively analyzed clinical characteristics, profiles malignant diseases infections Department Hematology, Nanfang Hospital, Southern Medical University. Results: A total 582 incidences occurred 22,717 inpatients. rates were 2.79%, 2.99%, 2.02%, respectively. Five hundred...
Survivin is an essential component of the chromosomal passenger complex and a member inhibitor apoptosis family. It expressed at high levels in large variety malignancies, where it has been implicated drug resistance. was also shown previously that survivin up-regulated during telomerase-mediated immortalization, which occurs relatively early stage carcinogenesis. This study shows up-regulation immortalization human myofibroblasts indirect consequence repression p16INK4a. p16INK4a were...
Umbilical cord blood (UCB) transplantation can provide a successful therapeutic option for patients that have no suitable related donor. UCB is often limited by the relatively small hematopoietic stem cell (HSC) numbers in especially adult recipients. Early neutrophil and platelet engraftment correlates with transplant. Compared to other HSC sources, immune reconstitution following transplant slower complicated increased frequency of opportunistic infections. The effect on was not thoroughly...
<title>Abstract</title> <bold>Background:</bold> Previous research has indicated that the administration of short-acting sedatives prior to weaning from mechanical ventilation is linked a more rapid recovery and extubation process, as well lower intensive care unit (ICU) treatment expenses. The present study endeavors assess efficacy safety sequential application remimazolam besylate compared with midazolam before ventilation. <bold>Methods:</bold> This multicenter, randomized controlled...