A5078259046- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Virus-based gene therapy research
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- TGF-β signaling in diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Fault Detection and Control Systems
- Animal Genetics and Reproduction
- Immune cells in cancer
- Polysaccharides and Plant Cell Walls
- Angiogenesis and VEGF in Cancer
- Gene expression and cancer classification
- Immune Response and Inflammation
- Mast cells and histamine
- CAR-T cell therapy research
- Protease and Inhibitor Mechanisms
- Bone Metabolism and Diseases
National Research Council Canada
2003-2025
University of California, San Diego
2022
Universität Hamburg
2021
Hamburg University of Technology
2021
University of Chicago
2021
Biotechnology Research Institute
2003-2007
Université de Montréal
2006
National Academies of Sciences, Engineering, and Medicine
2004
Agriculture and Agri-Food Canada
2000
Centre National de la Recherche Scientifique
1998
The growing biopharmaceutical industry has reached a level of maturity that allows for the monitoring numerous key variables both process characterization and outcome predictions. Sensors were historically used in order to maintain an optimal environment within reactor optimize performance. However, technological innovation pushed towards on-line situ continuous quality attributes could previously only be estimated off-line. These new sensing technologies when coupled with software models...
ABSTRACT The potential of Lactobacillus rhamnosus R for producing exopolysaccharide (EPS) when grown on basal minimum medium supplemented with glucose or lactose was investigated. EPS production by L. is partially growth associated and about 500 mg per liter synthesized both sugars. product yield coefficient ( Y EPS/S ) 3.15 (0.0315 g [g lactose] −1 2.88 (0.0288 glucose] ). It clearly shown that the amount produced declined upon prolonged fermentation. Degradation in fermentation processes...
Abstract Protein expression from stably transfected Chinese hamster ovary (CHO) clones is an established but time‐consuming method for manufacturing therapeutic recombinant proteins. The use of faster, alternative approaches, such as non‐clonal stable pools, has been restricted due to lower productivity and longstanding regulatory guidelines. Recently, the performance pools improved dramatically, making them a viable option quickly producing drug substance GLP‐toxicology early‐phase clinical...
Shake flasks are a foundational tool in early process development by allowing high throughput exploration of the design space. However, lack online data at this scale can hamper rapid decision making. Oxygen transfer rate (OTR) monitoring has been readily applied as an characterization benchtop bioreactor scale. Recent advances modern sensing technology have allowed OTR to be available shake flask level. It is now possible multiplex time-of-action (e.g., Induction, temperature shift, pH...
Abstract Large‐scale transient transfection of mammalian cells is a recent and powerful technology for the fast production milligram amounts recombinant proteins (r‐proteins). As many r‐proteins used therapeutic structural studies are naturally secreted or engineered to be secreted, cost‐effective serum‐free culture medium that allows their efficient expression purification required. In an attempt design such medium, effect nine protein hydrolysates on cell proliferation, efficiency,...
Abstract Gene expression by large‐scale transfection of mammalian cells is becoming an established technology for the fast production milligram and even gram amounts recombinant proteins (r‐proteins). However, efforts are still needed to optimize parameters in order maximize volumetric productivities while maintaining product quality. In this study, efficiency productivity following transient gene HEK293 were evaluated using green fluorescent protein (GFP) human placental secreted alkaline...
Abstract Interleukin (IL) 4 induces IL 6 production by human umbilical vein endothelial cells (HUVEC) in a dose‐dependent manner, as shown bioassay and immunoprecipitation. Interferon (IFN)‐γ, which antagonizes effects on leukocytes, synergized with the induction of HUVEC. Contamination endotoxin was excluded heat‐inactivated 4, preincubating anti‐IL polyclonal antibody use polymyxin B. The presence receptors HUVEC affinity cross‐linking 125 I‐IL revealing 110‐kDa binding protein. However,...
One strategy to enhance the production of biological therapeutics is using transient perfusion in preculture (N-1 stage) seed culture (N at ultra-high cell densities (>10 x 106 viable cells/mL). This very high seeding density improves performance by shortening timeline and/or achieving higher final product concentrations. Typically, an N-1 train employs bioreactors with alternating tangential flow filtration (ATF) or (TFF) systems Wave bag bioreactor integrated membrane, which have costs and...
Transforming growth factor-β (TGF-β) plays a causal role in several human pathologies including fibrotic diseases and metastasis. TGF-β signaling is mediated through its interaction with three types of cell surface receptors, RI, RII, RIII. The soluble ectodomains RII RIII bind to TGF-β, making them attractive candidates sequester inhibit activity. To optimize the activity ectodomains, we studied effect artificially dimerizing upon their kinetics binding using an optical biosensor...
Abstract The recent COVID‐19 pandemic revealed an urgent need to develop robust cell culture platforms which can react rapidly respond this kind of global health issue. Chinese hamster ovary (CHO) stable pools be a vital alternative quickly provide gram amounts recombinant proteins required for early‐phase clinical assays. In study, we analyze early process development data trimeric spike protein Cumate‐inducible manufacturing platform utilizing CHO pool as preferred production host across...
Abstract Technology scale‐up and transfer are a fundamental critical part of process development in biomanufacturing. Important bioreactor hydrodynamic characteristics such as working volume, overhead gas flow rate, volumetric power input (P/V), impeller type, agitation regimen, sparging aeration strategy, sparger k L must be selected based on key performance indicators (KPI) to ensure smooth seamless transfer. Finding suitable operational setpoints developing an efficient feeding regimen...
Background: Chinese hamster ovary (CHO) cells are extremely important host for recombinant DNA technology with their utility requiring optimization of growth. The ability to test conditions using in silico models growing CHO can help advance the bioreactor towards higher viable cell concentration and increased mAb production. Methods: A new kinetic model metabolism is presented, tested provided this publication. RNASeq data from was used guide selection major metabolic pathways that were...