A5100730150- Neonatal Respiratory Health Research
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- RNA Research and Splicing
- Respiratory viral infections research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Diverse Music Education Insights
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- Education, Safety, and Science Studies
- Animal Disease Management and Epidemiology
- Advanced Polymer Synthesis and Characterization
- Diverse Approaches in Healthcare and Education Studies
- Pickering emulsions and particle stabilization
- Surfactants and Colloidal Systems
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Inhalation and Respiratory Drug Delivery
- Mosquito-borne diseases and control
- Cancer-related molecular mechanisms research
- HIV Research and Treatment
- Ovarian cancer diagnosis and treatment
- Viral Infections and Immunology Research
- Safe Handling of Antineoplastic Drugs
Chungnam National University
2021-2024
Korea Institute of Toxicology
2021-2024
Korea Disease Control and Prevention Agency
2024
Korea National Institute of Health
2024
Pusan National University
2024
Dankook University
2022-2023
Ajou University
2023
Chonnam National University
2021
Seoul National University
2018
Korea Advanced Institute of Science and Technology
2012
Polyhexamethylene guanidine phosphate (PHMG-p), a humidifier disinfectant, is known to cause lung toxicity, including inflammation and pulmonary fibrosis. In this study, we aimed investigate the effect of PHMG-p on human tissue models (2D epithelial cells 3D organoids) under conditions oxidative stress viral infection. The was studied by evaluating formation granules (SGs), which play pivotal role in cellular adaptation various conditions. Under respiratory syncytial virus (RSV) infection,...
Hand-foot-and-mouth disease (HFMD) is a viral infectious that occurs in children under 5 years of age. Its main causes are coxsackievirus (CV) and enterovirus (EV). Since there no efficient therapeutics for HFMD, vaccines effective preventing the disease. To develop broad coverage against CV EV, development bivalent vaccine form needed. The Mongolian gerbil an suitable animal model EV71 C4a CVA16 infection used to investigate efficacy following direct immunization. In this study, gerbils...
Abstract The micellization properties and phase behaviour of model ternary surfactant—oil—water systems were studied by grand canonical Monte Carlo simulations assisted histogram reweighting techniques. Larson's lattice was used for a symmetrical H4T4 surfactant, where H represents hydrophilic T hydrophobic groups. In contrast to earlier studies, oil chains variable length, from T4, the effect added on critical micelle concentration (cmc) determined quantitatively. It found that cmc...
Aim The role of feedback processing in decision‐making has been assessed psychiatric patients using the I owa G ambling T ask ( IGT ). Although impaired performance on documented extensively schizophrenia patients, neuropsychological mechanisms underlying deficits have not yet elucidated. Therefore, aim this study was to investigate origins various versions . Methods Thirty chronic and 33 healthy subjects underwent computerized , V ariant VGT ), S huffled SGT ) assess contributions...
The emergence of drug-resistant Mycobacterium tuberculosis ( M . tb ) has led to the development novel anti-tuberculosis (anti-TB) drugs. Common methods for testing efficacy new drugs, including two-dimensional cell culture models or animal models, have several limitations. Therefore, an appropriate model representative human organism is required. Here, we developed infection using lung organoids (hLOs) and demonstrated that H37Rv can infect epithelial cells macrophages (hMφs) in hLOs. This...
Hepatitis B virus (HBV), particularly through the HBx protein, induces oxidative stress during liver infections. This study reveals that increases reactive oxygen species (ROS) via two distinct mechanisms. The first mechanism is p53-independent, likely involving mitochondrial dysfunction, as demonstrated by elevated ROS levels in p53-deficient Hep3B cells and p53-knocked-down HepG2 after expression or HBV infection. increase persisted even when p53 transcriptional activity was inhibited...
Under various cellular stress conditions, including exposure to toxic chemicals, RNA-binding proteins (RBPs), Ras GTPase-activating protein-binding protein 1 (G3BP1), aggregate and form granule complexes, which serve as hallmarks of stress. The existing methods for analyzing assembly have limitations in the rapid detection dynamic ignore effects constitutively overexpressed RBP on stress-related processes. Therefore, overcome these limitations, we established a G3BP1-GFP reporter human lung...
Ovarian granulosa cell tumor (OGCT) is a rare ovarian that accounts for about 2-5% of all tumors. Despite the low grade tumors, high and late recurrences are common in OGCT patients. Even though this usually occurs adult women with estrogen levels, cause still unknown. To screen genetic variants associated OGCT, we collected normal matched-tumor formalin-fixed paraffin-embedded (FFPE) from 11 patients performed whole-exome sequencing (WES) using Illumina NovaSeq 6000. A total 1,067,219...
Efficient gene editing technology is critical for successful knock-in in domestic animals. RAD51 recombinase (RAD51) plays an important role strand invasion during homologous recombination (HR) mammals, and regulated by checkpoint kinase 1 (CHK1) CHK2 genes, which are upstream elements of (RAD51). In addition, mismatch repair (MMR) system inextricably linked to HR-related pathways regulates HR via heteroduplex rejection. Thus, the aim this study was investigate whether clustered regularly...
Nipah virus (NiV), of the Paramyxoviridae family, causes highly fatal infections in humans and is associated with severe neurological respiratory diseases. Currently, no commercial vaccine available for human use. Here, eight structure-based mammalian-expressed recombinant proteins harboring NiV surface proteins, fusion glycoprotein (F), major attachment (G) were produced. Specifically, prefusion NiV-F and/or NiV-G glycoproteins expressed monomeric, multimeric (trimeric F tetra G), or...
Chikungunya fever is a mosquito-borne infectious disease caused by the chikungunya virus (CHIKV). Recently, CHIKV has spread rapidly worldwide, raising global concerns. However, there only one approved vaccine available to prevent infection; therefore, different platform vaccines development public health priority. The genome encodes four non-structural polyproteins (nsP1-4) and structural polyprotein (capsid, envelope 3, 2, 6 K, 1). Previous studies have shown that N-linked glycans in viral...
Cells regulate protein synthesis under stressful circumstances by forming cytoplasmic RNA granules, termed stress granules (SGs). SGs are membrane-less organelles that function as a protective mechanism in response to stress. They through liquid-liquid phase separation, which is vital process comprising 2 distinct de-mixed liquid phases. The components of SGs, such G3BP1, can serve biomarkers cell toxicity. Respiratory diseases among the leading causes death globally. After humidifier...
Exposure to atmospheric particulate matter (PM) increases morbidity and mortality in respiratory diseases by causing various adverse health effects; however, the effects on cellular stress caused PM exposure virus infected conditions remain unclear. The of under 10 microns (PM ) diesel (DPM) syncytial (RSV) infection were investigated human two-dimensional lung epithelial cells three-dimensional organoids (hLOs) mimicking tissue evaluating formation granules (SGs), which play a pivotal role...
Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in.Rad51 mainly involved in homologous recombination and associated with strand invasion.The HR-related mismatch repair system maintains fidelity by heteroduplex rejection repair.Therefore, purpose this study to control Rad51, which plays critical role HR, through UV-induced DNA damage.It also confirm effect on expression MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) closely treatment inhibitor...