A5067374817- Genomics and Chromatin Dynamics
- Bioinformatics and Genomic Networks
- Genomics and Phylogenetic Studies
- Contact Dermatitis and Allergies
- Skin and Cellular Biology Research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Pregnancy and preeclampsia studies
- Genomic variations and chromosomal abnormalities
- Prostate Cancer Treatment and Research
- Genetics, Bioinformatics, and Biomedical Research
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Cancer Genomics and Diagnostics
- Salivary Gland Disorders and Functions
- Gene expression and cancer classification
- Traditional Chinese Medicine Analysis
- Mast cells and histamine
- Customer churn and segmentation
- Complex Network Analysis Techniques
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- Cancer, Lipids, and Metabolism
Shenyang University of Technology
2024-2025
Shenzhen Maternity and Child Healthcare Hospital
2008-2024
Southern Medical University
2018-2024
ShanghaiTech University
2024
Weill Cornell Medicine
2019-2024
Cornell University
2019-2024
Southwest Medical University
2021-2023
Tongji Hospital
2023
Huazhong University of Science and Technology
2023
Kanagawa Dental University
2021
In castration-resistant prostate cancer (CRPC), the loss of androgen receptor (AR) dependence leads to clinically aggressive tumors with few therapeutic options. We used ATAC-seq (assay for transposase-accessible chromatin sequencing), RNA-seq, and DNA sequencing investigate 22 organoids, six patient-derived xenografts, 12 cell lines. identified well-characterized AR-dependent neuroendocrine subtypes, as well two AR-negative/low groups: a Wnt-dependent subtype, stem cell-like (SCL) subtype...
One of the most abundant proteins in human saliva, mucin-7, is encoded by MUC7 gene, which harbors copy number variable subexonic repeats (PTS-repeats) that affect size and glycosylation potential this protein. We recently documented adaptive evolution variation among primates. Yet, genetic humans remained unexplored. Here, we found PTS-repeat has evolved recurrently lineage, thereby generating multiple haplotypic backgrounds carrying five or six alleles. Contrary to previous studies, no...
The polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder. MicroRNAs negatively regulate the expression of target genes at posttranscriptional level by binding to 3′ untranslated region genes. Our previous study showed that miR‐141‐3p was dramatically decreased in ovaries rat PCOS models. In this study, we aimed characterize ovarian granulosa cells. 3‐(4,5‐Dimethylthiazol‐2‐Yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) assay cell viability increased when...
Human skin has evolved rapidly, leaving evolutionary signatures in the genome. The filaggrin (FLG) gene is widely studied for its skin-barrier function humans. extensive genetic variation this gene, especially common loss-of-function (LoF) mutations, been established as primary risk factors atopic dermatitis. To investigate evolution of we analyzed 2,504 human genomes and genotyped copy number repeats within FLG 126 individuals from diverse ancestral backgrounds. We were unable to replicate...
A common, 32kb deletion of LCE3B and LCE3C genes is strongly associated with psoriasis. We recently found that this ancient, predating Human-Denisovan divergence. However, it was not clear why negative selection has removed from the population.Here, we show haplotype block harbors (i) retains high allele frequency among extant ancient human populations; (ii) unusually nucleotide variation (π, P < 4.1 × 10-3); (iii) contains an excess intermediate variants (Tajima's D, 3.9 (iv) long time to...
Dioscin is a natural steroid saponin derived from several plants that shows potent anticancer effects against variety of cancer cells. Here, we investigated the antitumor effect dioscin human colon cells and evaluated molecular mechanism involved in this process. The cell cytotoxicity was studied by MTT assay BrdU incorporation. proapoptotic characterized flow cytometry analysis. A western blot an immunofluorescence staining were used to investigate how induces apoptosis vitro. In our study,...
Genomic structural variants constitute the majority of variable base pairs in primate genomes and affect gene function multiple ways. While whole duplications deletions are relatively well-studied, biology subexonic (i.e., within coding exon sequences), copy number variation remains elusive. The salivary MUC7 provides an opportunity for studying such variation, as it harbors repeat sequences that encode densely O-glycosylated domains (PTS-repeats) with microbe-binding properties. To...
Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder. To understand the pathogenesis of PCOS, we established rat models PCOS induced by letrozole employed deep sequencing to screen differential expression microRNAs (miRNAs) in rats control rats. We observed vaginal smear detected ovarian pathological alteration hormone level changes Deep showed that total 129 miRNAs were differentially expressed ovaries from letrozole-induced model compared with control,...
Complete placenta previa is associated with a higher percentage of adverse clinical outcomes and magnetic resonance imaging (MRI) widely used in the preoperative examination patients previa.To evaluate effectiveness placental area lower uterine segment cervical length identifying maternal-fetal women complete previa.Retrospective.A total 141 pregnant (median age, 32; age range, 24-40 years) were examined by MRI to uteroplacental condition.A 3 T T1 -weighted (T1 WI), T2 (T2 half-Fourier...
Regulatory networks containing enhancer-gene edges define cellular states. Multiple efforts have revealed these for reference tissues and cell lines by integrating multi-omics data. However, the methods developed cannot be applied large patient cohorts due to infeasibility of chromatin immunoprecipitation sequencing (ChIP-seq) limited biopsy material. We trained machine-learning models using interaction analysis with paired-end tag (ChIA-PET) high-throughput chromosome conformation capture...
Water is a vital resource, and its quality has direct impact on human health. Groundwater, as one of the primary water sources, requires careful monitoring to ensure safety. Although manual methods for testing are accurate, they often time-consuming, costly, inefficient when dealing with large complex data sets. In recent years, machine learning become an effective alternative assessment. However, current approaches still face challenges, such limited performance individual models, minimal...
Constructing alignments and phylogenies for a given locus from large genome sequencing studies with relevant outgroups allow novel evolutionary anthropological insights. However, no user-friendly tool has been developed to integrate thousands of recently available anthropologically sequences construct complete sequence phylogenies.Here, we provide VCFtoTree, user friendly graphical interface that directly accesses online databases download, parse analyze variation data regions interest. Our...
The third most often diagnosed disease globally and the second prevalent cause of cancer-related death is colorectal cancer (CRC). Numerous human malignancies have been identified to high expression ADORA2A. However, it still ambiguous about its function in CRC. RNA-seq with stable transfected SETDB1 knockdown cells was used identify differentially expressed genes. Further, ADORA2A CRC cell lines SW620 HCT116 performed siRNA over SW480 conducted plasmids. CCK8, colony formation, wound...
Plants have evolved a large number of transcription factors (TF), which are enriched among duplicate genes, highlighting their roles in complex regulatory networks. The APETALA2/EREBP-like genes constitute plant TF family and participate development stress responses. To probe the conservation divergence AP2/EREBP we analyzed duplication patterns this Brassicaceae identified interacting proteins representative Arabidopsis proteins. We found that many duplicates generated early history were...
Colorectal cancer (CRC) is one of the most common malignant cancers in human, and its incidence increases gradually every year. Metastasis an important factor leading to tumor development. The epithelial-mesenchymal transition (EMT) has been proved be closely related metastasis, yet mechanism CRC remains explored.We obtained differentially expressed gene C5aR1 with SETDB1 stable overexpression knockdown cells by RNA-seq. Cell proliferation was tested CCK8 colony formation assay. Migration...
Abstract Background Chromatin regulators (CRs) are critical epigenetic modifiers and have been reported to play roles during the progression of various tumors, but their role in lung adenocarcinoma (LUAD) has not comprehensively studied. Methods Differential expression univariate Cox regression analyses were conducted identify prognostic CRs. Consensus clustering was applied classify subtypes LUAD based on LASSO-multivariate method used for construction a signature development chromatin...
ABSTRACT Bipolar disorder is a highly heritable mental illness, but the relevant genetic variants and molecular mechanisms are largely unknown. Recent GWASs have identified an intergenic region associated with both cognitive performance bipolar disorder. This contains dozens of putative fetal brain-specific enhancers located ∼0.7 Mb upstream neuronal transcription factor POU3F2 . We candidate causal variant, rs77910749, that falls within conserved enhancer, LC1. human-specific variant...
Previous studies have surveyed the potential impact of loss-of-function (LoF) variants and identified LoF-tolerant protein-coding genes. However, tolerance human genomes to losing enhancers has not yet been evaluated. Here we present catalog using structural from whole-genome sequences. Using a conservative approach, estimate that individual possess at least 28 on average. We assessed properties in unified regulatory network constructed by integrating tissue-specific gene-gene interactions....
This study aimed to determine expressions of methyltransferase-like 3 (METTL3) and METTL14, two enzymes essential for mRNA methylation at the adenosine (m6 A), in oral squamous cell carcinoma (OSCC) investigate vitro aggressiveness their aberrant expressions.METTL3 METTL14 50 OSCC 11 normal tissues were examined by immunohistochemistry. METTL3 m6 A amounts determined three lines, including HN5, HN6, HN15. Cell proliferation, migration, invasion studied BrdU, wound healing, Transwell chamber...