A5035951730- Effects of Radiation Exposure
- Radiation Therapy and Dosimetry
- Advanced Radiotherapy Techniques
- DNA Repair Mechanisms
- Chemotherapy-induced cardiotoxicity and mitigation
- Advanced X-ray and CT Imaging
- Lung Cancer Diagnosis and Treatment
- Cardiac Imaging and Diagnostics
- Nanoparticle-Based Drug Delivery
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- PARP inhibition in cancer therapy
- Ultrasound and Hyperthermia Applications
- Nanoplatforms for cancer theranostics
- Radiation Dose and Imaging
- Radiopharmaceutical Chemistry and Applications
- Cell death mechanisms and regulation
- Advances in Oncology and Radiotherapy
- Cardiovascular Function and Risk Factors
- Digital Radiography and Breast Imaging
- Carcinogens and Genotoxicity Assessment
- PI3K/AKT/mTOR signaling in cancer
- Advanced Surface Polishing Techniques
- MRI in cancer diagnosis
- DNA and Nucleic Acid Chemistry
Queen's University Belfast
2016-2025
University of Manchester
2024
National Physical Laboratory
2020
University of Surrey
2020
The Northern Ireland Cancer Centre
2020
Nestlé (France)
2020
Paul Scherrer Institute
2016
Virginia Commonwealth University Medical Center
2016
Radiation therapy is one of the most common and effective strategies used to treat cancer. The irradiation usually performed with a fractionated scheme, where dose required kill tumour cells given in several sessions, spaced by specific time intervals, allow healthy tissue recovery. In this work, we examined DNA repair dynamics exposed radiation delivered fractions, assessing response histone-2AX (H2AX) phosphorylation (γ-H2AX), marker double strand breaks. γ-H2AX foci induction...
Irradiation with ultra-high dose rate (FLASH) has been shown to spare normal tissue without hampering tumor control in several vivo studies. Few cell lines have investigated vitro, and previous results are inconsistent. Assuming that oxygen depletion accounts for the FLASH sparing effect, no should appear cells irradiated low doses normoxia.Seven cancer (MDA-MB-231, MCF7, WiDr, LU-HNSCC4, HeLa [early passage subclone]) lung fibroblasts (MRC-5) were ranging from 0 12 Gy using (≥800 Gy/s) or...
hSSB1 is a newly discovered single-stranded DNA (ssDNA)-binding protein that essential for efficient double-strand break signalling through ATM. However, the mechanism by which functions to allow unknown. Here, we show recruited rapidly sites of breaks (DSBs) in all interphase cells (G1, S and G2) independently of, CtIP, MDC1 MRN complex (Rad50, Mre11, NBS1). However expansion from DSB site requires function MRN. Strikingly, silencing prevents foci formation as well recruitment DSBs leads...
Abstract Gold nanoparticles (GNPs) have been demonstrated as effective radiosensitizing agents in a range of preclinical models using broad field sources various energies. This study aimed to distinguish between these mechanisms by applying subcellular targeting soft X-ray microbeam combination with GNPs. DNA damage and repair kinetics were determined following nuclear cytoplasmic irradiation (carbon K-shell, 278 eV) MDA-MB-231 breast cancer AG01522 fibroblast cells without To investigate...
The recent implementation of MR-Linacs has highlighted theranostic opportunities contrast agents in both imaging and radiotherapy. There is a lack data exploring the potential superparamagnetic iron oxide nanoparticles (SPIONs) as radiosensitisers. Through preclinical 225 kVp exposures, this study aimed to characterise uptake radiobiological effects SPIONs tumour cell models vitro provide proof-of-principle application xenograft model.
Gold nanoparticles have attracted significant interest in cancer diagnosis and treatment. Herein, we evaluated the theranostic potential of dithiolated diethylenetriamine pentaacetic acid (DTDTPA) conjugated AuNPs (Au@DTDTPA) for CT-contrast enhancement radiosensitization prostate cancer.In vitro assays determined Au@DTDTPA uptake, cytotoxicity, radiosensitizing DNA damage profiles. Human PC3 xenograft tumor models were used to determine CT radiation modulating effects vivo.Cells exposed...
To fully exploit the prospects presented by increasing focus on biological approaches for enhancing radiotherapy outcomes, improvements in repeatability and translatability of radiobiological preclinical studies are required. This requires development adoption appropriate dosimetric standards reproducible to increase confidence studies, enabling inter-laboratory validation facilitating clinical translation. An IPEM Working-Party reviewed current status challenges associated with dosimetry...
Abstract Introduction: Improvements in preclinical radiation research have been made to better mimic the equipment and techniques implemented clinic. The development of dedicated small animal units facilitates such advances by combining treatment planning, image guidance conformal delivery. One area significantly behind its clinical equivalent are standardised dosimetry quality assurance (QA) protocols, hampering translatability results into interventions. 
Approach: aim study...
Preclinical radiation biology has become increasingly sophisticated due to the implementation of advanced small animal image guided platforms into laboratory investigation. These radiotherapy devices enable state-of-the-art therapy (IGRT) research be performed by combining high-resolution cone beam computed tomography (CBCT) imaging with an isocentric irradiation system. Such are capable replicating modern clinical systems similar those that integrate a linear accelerator on-board CBCT...
Background and purposeRadiation induced cardiotoxicity (RICT) is as an important sequela of radiotherapy to the thorax for patients. In this study, we aim investigate dose fractionation response RICT. We propose global longitudinal strain (GLS) early indicator RICT myocardial deformation following irradiation.MethodsRICT was investigated in female C57BL/6J mice which base heart irradiated under image-guidance using a small animal radiation research platform (SARRP). Mice were randomly...
Abstract In advanced radiotherapy, intensity modulated radiation fields and complex dose-delivery are utilized to prescribe higher doses tumours. Here, we investigated the impact of on radio-sensitivity cell recovery during dose delivery. We generated experimental survival data after single-dose, split-dose fractionated irradiation in normal human skin fibroblast cells (AGO1522) prostate cancer (DU145). The was delivered either 50% area a T25 flask containing (half-field) or 100%...
DNA damage (caused by direct cellular exposure and bystander signaling) the complex pathways involved in its repair are critical events underpinning tissue response following radiation exposures. There limited data addressing dynamics of induction skin particularly areas not directly exposed. Here we investigate mechanisms regulating damage, repair, intracellular signalling their impact on premature differentiation development inflammatory-like irradiated surrounding a 3D organotypic model....
Radiomics image analysis has the potential to uncover disease characteristics for development of predictive signatures and personalised radiotherapy treatment. Inter-observer inter-software delineation variabilities are known have downstream effects on radiomics features, reducing reliability analysis. The purpose this study was investigate impact these outputs from preclinical cone-beam computed tomography (CBCT) scans. were assessed using manual semi-automated contours mouse lungs (n =...
Radiotherapy is planned to achieve the optimal physical dose distribution target tumour volume whilst minimizing surrounding normal tissue. Recent in vitro experimental evidence has demonstrated an important role for intercellular communication radiobiological responses following non-uniform exposures. This study aimed model impact of these effects context techniques involving highly modulated radiation fields or spatially fractionated treatments such as radiotherapy (GRID).Using...
Advanced radiotherapy techniques such as intensity-modulated radiation therapy (IMRT) achieve high levels of conformity to the target volume through sequential delivery highly spatially and temporally modulated fields, which have been shown impact radiobiological response. This study aimed characterize time cell type dependency survival responses fields using single (SCT) mixed (MCT) co-culture models transformed fibroblast (AG0-1522b) cells, prostate (DU-145) lung (H460) cancer cells. In...