A5035796877- Helicobacter pylori-related gastroenterology studies
- Bone Metabolism and Diseases
- Heat shock proteins research
- Toxin Mechanisms and Immunotoxins
- Galectins and Cancer Biology
- Plant biochemistry and biosynthesis
- Bone health and treatments
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Veterinary medicine and infectious diseases
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme function and inhibition
- Photosynthetic Processes and Mechanisms
- Gastric Cancer Management and Outcomes
- NF-κB Signaling Pathways
- S100 Proteins and Annexins
- Bone and Dental Protein Studies
- Signaling Pathways in Disease
- Catalysis for Biomass Conversion
- melanin and skin pigmentation
- Mycobacterium research and diagnosis
- ATP Synthase and ATPases Research
- Skin Protection and Aging
- Beetle Biology and Toxicology Studies
National Institute for Communicable Disease Control and Prevention
2016-2025
Chinese Center For Disease Control and Prevention
2012-2020
Center for Disease Control
2012-2020
Harbin Institute of Technology
2016-2018
State Key Laboratory of Plant Genomics
2015
Institute of Genetics and Developmental Biology
2015
Chinese Academy of Sciences
2007-2015
Institute of Biophysics
2007-2010
Emory University
1997-2001
Veterans Health Administration
1999
The mechanisms by which glucocorticosteroids promote osteoclastogenesis in vitro are uncertain. As macrophage colony-stimulating factor (MCSF) is critical for osteoclastogenesis, we hypothesized that might regulate membrane-bound MCSF (mMCSF) and soluble (sMCSF) production stromal cells or osteoblasts. ST2 murine calvarial osteoblasts (MOBs) were treated with dexamethasone (Dex; 100 nm) and/or 1,25-dihydroxyvitamin D[ 1,25(OH)2D; 10 nm] 3 days. Control values mMCSF sMCSF as units per 100,000...
Lung cancer (LC) remains the leading cause of death globally. Recent reports have suggested that circulating microbial nucleic acids potential as promising biomarkers for liquid biopsies. However, profiles and their clinical value in LC patients remained unexplored. In this study, plasma samples from 76 patients, 9 liver 11 pancreatic 53 healthy controls (HCs) were collected underwent metagenomic analyses by whole genome sequencing. The composition relative abundance significantly different...
Abstract Background There are geographic variations in the genotypes of Helicobacter pylori ( H. ) cagA , vacA iceA oipA and dupA . The aim study was to investigate distribution these among strains from five regions China their association with clinical outcomes. Materials methods Gastric biopsy specimens were obtained 348 patients different gastrointestinal diseases China. regional 89 Shandong, 91 Guangxi, 57 Hunan, 58 Qinghai 53 Heilongjiang. presence determined by polymerase chain...
Abstract When assessing a correlation between two exposure or biological marker variables, one sometimes encounters the problem of indeterminate values for variables due to an assay detection limit. In this event, investigators often report coefficients computed after removing pairs involving non‐detectable values, substituting some small constant those values. These ad hoc practices can lead bias in both point and confidence interval estimates true coefficient. To address issue, we consider...
Macrophage colony-stimulating factor (MCSF), although necessary for entry of precursors into the early preosteoclast pathway, inhibits osteoclastogenesis at high doses. To clarify relationship between MCSF and osteoclast formation, we investigated effect exogenous in murine bone marrow culture. Precursor proliferation expression MCSF-receptor were examined after 4 days culture presence or absence accessory stromal cells. In both mixed destromalized cell cultures, dose-dependently decreased...
ABSTRACT Background Metronidazole is a first‐line antibiotic to treat Helicobacter pylori infections. However, the Clinical Laboratory Standards Institute guidelines recommend against using antimicrobial susceptibility test (AST) metronidazole resistance, due unreliable predictive power which can result in treatment failure. Objectives The aim of this study was establish an 8‐h, metabolic‐phenotype based AST for H . D 2 O‐probed Raman microspectroscopy. Methods Minimal inhibitory...
Helicobacter pylori (H. pylori) adhesion to human gastric epithelial cells is closely linked with fucosylated glycans. Therefore, investigation of fucosylation in the interaction H. critical. In this study we used lectin microarrays detect expression glycans (GES-1) infected strains isolated from patients different diseases including chronic gastritis, duodenal ulcers, and cancer (each containing two strains) at 4 h. addition, investigated time-course fucosyltransferase (FUT) 1–6 genes GES-1...
Trigger factor (TF) is the first chaperone to interact with nascent chains and facilitate their folding in bacteria. Escherichia coli TF 432 residues length contains three domains distinct structural functional properties. The N-terminal domain of important for ribosome binding, M-domain carries PPIase activity. However, function C-terminal remains unclear, or regions directly involved substrate binding have not yet been identified. Here, a hydrophobic probe, bis-ANS, was used characterize...
Abstract Carbonic anhydrase II (CA II) expression is vital to normal osteoclast function. We and others have previously reported induction of CA messenger RNA (mRNA) by 1,25(OH)2D3 in myelomonocytic cells marrow culture. However, since stimulates differentiation as well, we wished separate direct effects on the gene from differentiating hormone. Using primary murine mixed cultures, measured mRNA RT-PCR. 10 nm dose dependently induced (4.12 ± 0.68-fold) at day 4 culture compared with control...
Expression of MCSF in bone is important to the regulation osteoclastogenesis. We show here that tumor necrosis factor-α (TNFα) increases production both soluble (sMCSF) and membrane-bound (mMCSF) macrophage colony stimulating factor by ST2 stromal cells. Treatment cells with TNFα caused sMCSF levels increase 394 ± 5% from basal; mMCSF rose 316 66% 30 10 per 100,000 same time. These were consistent increased expression mRNAs encoding isoforms. Increases mRNA are also seen after stimulation...
Macrophage colony-stimulating factor (MCSF) and osteoprotegerin ligand (OPGL), both produced by osteoblasts/stromal cells, are essential factors for osteoclastogenesis. Whether local MCSF levels regulate the amount of osteoclast formation is unclear. Two culture systems, ST-2 Chinese hamster ovary-membrane-bound (CHO-mMCSF)-Tet-OFF were used to study role mMCSF in formation. Cells from bone marrow (BMM) or spleen cultured with soluble OPGL on glutaraldehyde-fixed cell layers; osteoclasts...
Trigger factor (TF) is a key component of prokaryotic chaperone network, which involved various basic cellular processes such as nascent peptide folding, protein trafficking, ribosome assembly. To better understanding the physiological roles TF, global transcriptome profiles variety TF deletion mutant strains Escherichia coli were determined. We found that tig gene, encoding led to dramatic alteration profile, not only affecting gene expression members but also changing levels quite few RNAs...