A5101424560- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Veterinary medicine and infectious diseases
- T-cell and B-cell Immunology
- Gastroesophageal reflux and treatments
- Chemokine receptors and signaling
- Radiopharmaceutical Chemistry and Applications
- Dietary Effects on Health
- Immune Response and Inflammation
- Microbial Metabolites in Food Biotechnology
- Gastrointestinal disorders and treatments
- Glycosylation and Glycoproteins Research
- Diabetes Treatment and Management
- Peptidase Inhibition and Analysis
- Eosinophilic Esophagitis
- Medical Imaging and Pathology Studies
- Biochemical and Molecular Research
- RNA Interference and Gene Delivery
- Mycobacterium research and diagnosis
- Brucella: diagnosis, epidemiology, treatment
- PI3K/AKT/mTOR signaling in cancer
- Cytokine Signaling Pathways and Interactions
Peking University
2022-2025
Peking University Third Hospital
2022-2025
National Institute for Communicable Disease Control and Prevention
2020-2021
Center for Disease Control
2020-2021
Chinese Center For Disease Control and Prevention
2017-2021
Shanxi Provincial Children's Hospital
2008
Bristol-Myers Squibb (United States)
2007
National Cancer Institute
2007
Abstract Introduction Helicobacter pylori infection is the main risk factor for gastric cancer. Chronic inflammation usually induced by H. and accompanied inherent immune disorders. However, dynamic changes in host response associated with transition from normal to metaplasia, dysplasia, cancer are largely undefined. Method We established mice model. The mucosa of infected were subjected RNA-sequencing analysis at different stages. analyzed systemic disturbances spleen serum inflammatory...
Abstract Background There are geographic variations in the genotypes of Helicobacter pylori ( H. ) cagA , vacA iceA oipA and dupA . The aim study was to investigate distribution these among strains from five regions China their association with clinical outcomes. Materials methods Gastric biopsy specimens were obtained 348 patients different gastrointestinal diseases China. regional 89 Shandong, 91 Guangxi, 57 Hunan, 58 Qinghai 53 Heilongjiang. presence determined by polymerase chain...
Abstract Luteolin (Lut) has been shown to inhibit gastric cancer (GC); however, its efficacy compared other clinical drugs not examined in human samples. This study aimed elucidate the antitumor activity of Lut GC patient‐derived organoids (PDOs). PDOs were established from tissues, and characterization tissues was performed using whole‐exome sequencing. Drug sensitivity tests by treating with Lut, norcantharidin (NCTD), carboplatin (CP). RNA sequencing mechanism which further verified three...
The dysfunction of innate immunity components is one the major drivers for ulcerative colitis (UC), and increasing reports indicate that gut microbiome serves as an intermediate between genetic mutations UC development. Here, we find IL-17 receptor subunit, CMTM4, reduced in patients DSS-induced colitis. deletion CMTM4 (Cmtm4-/-) mice leads to a higher susceptibility comparison wildtype, significantly changes composition. causal role confirmed with co-housing experiment. We further identify...
Helicobacter pylori (H. pylori) adhesion to human gastric epithelial cells is closely linked with fucosylated glycans. Therefore, investigation of fucosylation in the interaction H. critical. In this study we used lectin microarrays detect expression glycans (GES-1) infected strains isolated from patients different diseases including chronic gastritis, duodenal ulcers, and cancer (each containing two strains) at 4 h. addition, investigated time-course fucosyltransferase (FUT) 1–6 genes GES-1...
The noninvasive detection technique using serum for large-scale screening is useful the early diagnosis of gastric cancer (GC). Herein, we employed liquid chromatography mass spectrometry to determine proteome signatures and related pathways in individuals with precancerous (pre-GC) lesions GC explore effect Helicobacter pylori ( H. ) infection. Differentially expressed proteins pre-GC compared non-atrophic gastritis (NAG) group were identified. APOA4, a protein associated metaplastic...
Abstract Background The cytotoxin-associated gene A ( cagA ) is one of the most important virulence factors Helicobacter pylori H. ). There a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in C-terminal CagA protein. This thought to play an role pathogenesis gastrointestinal diseases. aim this study was investigate diversity 3′ variable and amino acid polymorphisms EPIYA segments , their association with gastroduodenal Methods total 515 strains from patients 14 different...
HpaA as an outer membrane protein of Helicobacter pylori ( H. ) plays a significant role in the adhesion to human stomach, but functional relation between and gastric epithelial cells is still not clear. To screen interaction cellular proteins cells, from 26695 fused with tag (6× His) was expressed purified successfully, secondary structure estimated by Circular Dichroism (CD) spectrum, recombinant used perform pull-down assays cancer cell lines (AGS SGC-7901) lysates, respectively. The were...
3506 Background: Insulin-like growth factor-1 receptor (IGF-1R) signaling is an important regulator of mitogenesis, transformation to the oncogenic phenotype and anti-apoptotic effects in malignant cells. Over-expression IGF-1R, seen many tumors, may confer a advantage or drug resistance. A potent small-molecule inhibitor (BMS-536924) IGF-1R tyrosine kinase showed anti-tumor activity sarcoma, prostate, colon pancreatic tumor models. One integral goals development BMS-536924 as cancer...
Some amoxicillin-resistant strains of H. pylori show a sharp decrease in amoxicillin resistance after freezing. In China, most clinical gastric mucosal specimens are frozen and transported for isolation drug susceptibility testing pylori, which may lead to an underestimation the resistance. The objective this study is investigated reasons decreased cryopreservation. A high-level clone (NX24r) was obtained through pressure screening. After cryopreservation at −80 °C 3 months, minimum...
CKLF-like MARVEL transmembrane domain-containing 4 (CMTM4) is involved in immune regulation and tumor progression; however, its role gastric cancer (GC) remains unclear. This study explored the mechanism of CMTM4 GC.
Abstract Background: There were geographical differences in the distribution of Helicobacter pylori ( H. ) genotypes cagA , vacA iceA oipA and dupA et al). The population different regions China have grant patterns gastroduodenal diseases which are associated with these genotypes, but characteristics still unknown. Materials Methods: Gastric biopsy specimens obtained from 348 patients five China. regional was 89 Shandong, 91 Guangxi, 57 Hunan, 58 Qinghai 53 Heilongjiang. DNA extracted...
Introduction Refractory peptic ulcers lead to perforation and hemorrhage, which are fatal. However, these remain a therapeutic challenge. Gastric mucosal blood flow is crucial in maintaining gastric health. It’s reported that Glucagon-like peptide-2 (GLP-2), gastrointestinal hormone, stimulated intestinal flow. the direct role of GLP-2 metabolites unclear. Here, we speculated might protect mucosa by increasing regulating metabolites. This study was conducted evaluate lesions its underlying...
Abstract Background: CagA is one of the most important virulence factors Helicobacter pylori ( H. ). There a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in 3’ variable region. This thought to play an role pathogenesis gastrointestinal diseases. The aim this study was investigate diversity and amino acid polymorphisms EPIYA segments, their association with gastroduodenal Methods: A total 515 isolates from patients 14 different geographical regions China were collected genomic...
Objective The survival and replication of two Brucella melitensis (B.melitensis) with different virulence in Raw 264.7 cell their ability inducing cytokine were compared to provide basis for establishing a method identify Brucella. Methods B.melitensis 16M M5 used infect cells the amount interleukin (IL)-1β, IL-6 IL-12 culture supernatants detected via enzyme-linked immunosorbent assay at 4, 8 24 h after infection. We also evaluated presence colony-forming units (CFU) 2, 8, 48...
Inflammatory bowel diseases (IBD) are chronic inflammatory affecting 1 in every 150 Canadians. In contrast to healthy individuals, IBD patients develop exaggerated responses against their resident microbiota, at the level of single layer epithelial cells (IEC) lining intestinal tract. Typically, innate immune hypo-responsiveness IEC prevents such maladaptive responses, part due strong expression Single Ig IL-1 related receptor (SIGIRR), a negative regulator interleukin (IL)-1 and toll-like...
Abstract Background: The cytotoxin-associated gene A ( cagA ) is one of the most important virulence factors Helicobacter pylori H. ). There a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in C-terminal CagA protein. This thought to play an role pathogenesis gastrointestinal diseases. aim this study was investigate diversity 3’ variable and amino acid polymorphisms EPIYA segments , their association with gastroduodenal Methods: total 515 strains from patients 14 different...