A5051340310- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Ocular Diseases and Behçet’s Syndrome
- Ocular Oncology and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Chemokine receptors and signaling
- Vasculitis and related conditions
- Reproductive System and Pregnancy
- Intraocular Surgery and Lenses
- Atherosclerosis and Cardiovascular Diseases
- Complement system in diseases
- Xenotransplantation and immune response
- Otitis Media and Relapsing Polychondritis
- Immunotherapy and Immune Responses
- Ocular Infections and Treatments
- interferon and immune responses
- Glaucoma and retinal disorders
- Platelet Disorders and Treatments
- Cutaneous Melanoma Detection and Management
- Retinal Imaging and Analysis
- Endometriosis Research and Treatment
- Immune cells in cancer
- Retinal and Macular Surgery
- Retinal Development and Disorders
- Glioma Diagnosis and Treatment
Copenhagen University Hospital
2016-2025
Rigshospitalet
2016-2025
University of Copenhagen
2016-2025
Glostrup Hospital
2014-2022
Purpose.: To investigate the effects of T-cell–derived cytokines on gene and protein expression chemokines in a human RPE cell line (ARPE-19). Methods.: We used an vitro coculture system which CD3/CD28–activated T-cells were separated by membrane. chemokine genes was quantified using three different types microarrays. Protein determined single multiplex ELISA immunoblotting. Results.: Coculture with activated increased mRNA CCL2 (MCP-1); CCL5 (RANTES); CCL7 (MCP-3); CCL8 (MCP-2); CXCL1...
Giant cell arteritis (GCA) is a medical and ophthalmological emergency due to risk of stroke sudden irreversible loss vision. Fast accurate diagnosis important prevent complications long-term high dose glucocorticoids toxicity. Temporal artery biopsy gold standard for diagnosing GCA. However, temporal ultrasound fast non-invasive procedure which may provide supplement or an alternative biopsy. This study assesses the diagnostic performance in Examination results patients suspected having GCA...
Abstract The prognostic value of metabolic tumor burden parameters obtained from 18 F-FDG PET/CT imaging was evaluated in this retrospective national multicenter study patients with metastatic posterior uveal melanoma (PUM) and compared to the largest diameter lesion (LDLM) American Joint Committee on Cancer (AJCC) staging system. Maximal Standard Uptake Value (SUV max ), Metabolic Tumor Volume (MTV), Total Lesion Glycolysis (TLG) were 106 patients. Higher values SUV ( p = 0.007, log-rank),...
Immune cells in primary uveal melanoma (PUM) have mostly been studied enucleated tissue, thereby precluding material from thinner, low-risk PUM tumors for research purposes. Here, we investigated the feasibility of using tumor tissue acquired by transvitreal retinochoroidal (TVRC) biopsies to study immune microenvironment and relation genetic risk class. Collected 41 patients were tested aberrations determine high-risk (n = 19), medium-risk 12), 9) digested flow cytometry analysis cell...
The chemokine receptor CXCR3 has been strongly related to inhibition of angiogenesis. purpose this study was investigate the association between expression on peripheral blood leukocytes and age-related wet macular degeneration. Furthermore, we measured plasma concentration chemokines CXCL9 -11.The group consisted patients with degeneration (AMD) attending our department. Patients referred for reasons other than AMD were enrolled as control subjects. T cells -11 using flow cytometry.We...
Purpose To investigate the effects of inflammatory factors and oxidative stress on cell survival human retinal pigment epithelial (RPE) line, ARPE-19. Methods Confluent RPE cells were treated with peripheral blood mononuclear cells-conditioned medium (PCM), H2O2, NaIO3, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, or combinations these. Cell viability was determined by assays light microscopy. Effector molecules death investigated immunofluorescence microscopy flow cytometry....
Uveal melanoma (UM) is the most common primary intraocular tumor in adults and presence of infiltrating leucocytes associated with a poor prognosis. Little known how influence cells. The purpose this study was to investigate effect activated T cells on expression chemotactic cytokines UM Furthermore, we examined ability stimulated attract monocytes.We used an vitro coculture system which cell lines were cultured together, but separated by membrane. gene quantified using microarray. Protein...
Purpose The chemokine receptors CX3CR1 and CCR2 have been implicated in the development of age-related macular degeneration (AMD). evidence is mainly derived from experimental cell studies murine models AMD. purpose this study was to investigate association between expression on different leukocyte subsets Furthermore we measured plasma levels ligands CX3CL1 CCL2. Methods Patients attending our department were asked participate study. diagnosis AMD based clinical examination multimodal...
Since bilateral simultaneous postoperative endophthalmitis (BSPOE) after immediate sequential cataract surgery (ISBCS) can be devastating for the patient, evaluating such cases in depth is important to maintaining patient safety.
Abstract Purpose We have recently identified homeostatic alterations in the circulating T cells of patients with age‐related macular degeneration ( AMD ). In cultures retinal pigment epithelial cells, we demonstrated that T‐cell‐derived cytokines induced upregulation complement, chemokines and other proteins implicated pathogenesis. The purpose this study was to test whether increased plasma levels were present . Methods conducted a case–control study. Age‐related status assessed using...
Purpose: To determine to which extent inflammatory cytokines affect chemokine secretion by primary human choroidal melanocytes (HCMs), their capacity attract monocytes, and whether HCMs are able influence the proliferation of activated T cells. Methods: Primary cultures were established from eyes 13 donors. Human stimulated with IFN-γ TNF-α or supernatant cells (T-cell–conditioned media [TCM]). Gene expression analysis was performed using microarrays. Protein levels quantified ELISA...
The aim of this study was to compare the functional and anatomical effectiveness photodynamic therapy (PDT) versus proton beam (PBT) in a real-life setting for treatment circumscribed choroidal hemangioma.A total 191 patients with diagnosis hemangioma treated by PBT or PDT were included analyses.The 119 (62.3%) compared 72 PBT. final best-corrected visual acuity did not differ significantly between two groups (P = 0.932) thickness lower group 0.001). None needed second-line therapy. In...
The complement system is closely linked to the pathogenesis of AMD. Several genes are expressed in RPE, and proteins accumulate drusen. Further, a common variant factor H (CFH) confers increased risk developing Because mechanisms by which changes function CFH influence development AMD unclear, we examined ocular expression as consequence age control null mutant mice.Gene neuroretinas RPE/choroid from young aged WT Cfh(-/-) C57BL/6J mice was analyzed microarrays. Expression wide range...
Age-related macular degeneration (AMD) has been associated with both systemic and ocular alterations of the immune system. In particular dysfunction complement factor H (CFH), a soluble regulator alternative pathway system, implicated in AMD pathogenesis. One ligands for CFH is long pentraxin 3 (PTX3), which produced locally retinal pigment epithelium (RPE). To test hypothesis that PTX3 relevant to immunohomeostasis may be pathogenesis, we measured plasma protein concentration analyzed...
Dysregulation of the CCR3/CCL11 pathway has been implicated in pathogenesis choroidal neovascularisation, a common feature late age-related macular degeneration (AMD). The aim this study was to investigate expression CCR3 and its ligand CCL11 peripheral blood patients with neovascular AMD.Patients AMD healthy controls were included. Blood samples obtained prepared for flow cytometry CCR3. Levels measured plasma using Cytometric Bead Array. Differences between groups tested Kruskal-Wallis...
The purpose of this study was to investigate seasonal variation in cases biopsy-proven GCA eastern Denmark a 29-year period.Pathology records all temporal artery biopsies between 1990 and 2018 were reviewed. For each patient, data collected which included age, sex, date birth biopsy result. Seasonality evaluated using logistic regression Poisson analysis. Lastly, an explorative pilot conducted possible association three weather parameters (average temperature, amount rain hours sunshine) the...