A5057049435- Single-cell and spatial transcriptomics
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Erythrocyte Function and Pathophysiology
- Acute Lymphoblastic Leukemia research
- Digital Imaging for Blood Diseases
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- vaccines and immunoinformatics approaches
- HER2/EGFR in Cancer Research
- Epigenetics and DNA Methylation
- Congenital Diaphragmatic Hernia Studies
- Pancreatic function and diabetes
- Eosinophilic Disorders and Syndromes
- Immune cells in cancer
Lund University
2016-2024
Abstract Cancer-associated fibroblasts (CAFs) are a major constituent of the tumor microenvironment, although their origin and roles in shaping disease initiation, progression treatment response remain unclear due to significant heterogeneity. Here, following negative selection strategy combined with single-cell RNA sequencing 768 transcriptomes mesenchymal cells from genetically engineered mouse model breast cancer, we define three distinct subpopulations CAFs. Validation at transcriptional...
Erythroid cell commitment and differentiation proceed through activation of a lineage-restricted transcriptional network orchestrated by group well characterized genes. However, the minimal set factors necessary for instructing red blood (RBC) development remains undefined. We employed screen transcription allowing direct lineage reprograming from fibroblasts to induced erythroid progenitors/precursors (iEPs). show that Gata1, Tal1, Lmo2, c-Myc (GTLM) can rapidly convert murine human...
Airway basal cells are crucial for regeneration of the human lung airway epithelium and believed to be important contributors chronic obstructive pulmonary disease (COPD) other disorders. To reveal how contribute discover novel therapeutic targets, these need further characterized. In this study, we optimized a flow cytometry-based cell sorting protocol primary dependent on size NGFR (nerve-growth factor receptor) expression. The population was found molecularly functionally heterogeneous,...
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link marker expression that of lineage-associated transcription factors (TFs) and identify GFRA2 BST1 as B cells. Functional analyses vitro vivo well single-cell gene supported these proteins defined distinct subpopulations include cells from both...
Knowledge of human fetal blood development and how it differs from adult is highly relevant to our understanding congenital immune disorders childhood leukemia, which the latter can originate in utero. Blood formation occurs waves that overlap time space, adding heterogeneity, necessitates single-cell approaches. Here, a combined immunophenotypic transcriptional map first trimester primitive presented. Using CITE-seq (cellular indexing transcriptomes epitopes by sequencing), molecular...
Abstract The advent of single cell (Sc) genomics has challenged the dogma haematopoiesis as a tree-like structure stepwise lineage commitment through distinct and increasingly restricted progenitor populations. Instead, analysis ScRNA-seq proposed that earliest events in human hematopoietic stem (HSC) differentiation are characterized by only subtle molecular changes, with cells (HSPCs) existing continuum low-primed cell-states gradually transition into specific (CLOUD-HSPCs). Here, we...
The emerging notion of hematopoietic stem and progenitor cells (HSPCs) as a low-primed cloud without sharply demarcated gene expression programs raises the question on how cellular-fate options emerge at which stem-like stage lineage priming is initiated. Here, we investigate single-cell chromatin accessibility Lineage-, cKit+, Sca1+ (LSK) HSPCs spanning early differentiation landscape. Application signal-processing algorithm to detect transition points corresponding massive alterations in...
The advent of tyrosine kinase inhibitors (TKIs) as treatment chronic myeloid leukemia (CML) is a paradigm in molecularly targeted cancer therapy. Nonetheless, TKI-insensitive stem cells (LSCs) persist most patients even after years and are imperative for disease progression well recurrence during treatment-free remission (TFR). Here, we have generated high-resolution single-cell multiomics maps from CML at diagnosis, retrospectively stratified by BCR::ABL1 IS (%) following 12 months TKI...
The advent of tyrosine kinase inhibitors (TKIs) as treatment chronic myeloid leukemia (CML) is a paradigm in molecularly targeted cancer therapy. Nonetheless, TKI-insensitive stem cells (LSCs) persist most patients even after years and are imperative for disease progression well recurrence during treatment-free remission (TFR). Here, we have generated high-resolution single-cell multiomics maps from CML at diagnosis, retrospectively stratified by BCR::ABL1 IS (%) following 12 months TKI...
The advent of tyrosine kinase inhibitors (TKIs) as treatment chronic myeloid leukemia (CML) is a paradigm in molecularly targeted cancer therapy. Nonetheless, TKI insensitive stem cells (LSCs) persist most patients even after years treatment. sustained presence, heterogeneity and evolvability LSCs are imperative for disease progression well recurrence during treatment-free remission (TFR). However, dynamic changes among LSC sub-populations upon therapy impede their measurement targeting....
The advent of tyrosine kinase inhibitors (TKIs) as treatment chronic myeloid leukemia (CML) is a paradigm in molecularly targeted cancer therapy. Nonetheless, TKI insensitive stem cells (LSCs) persist most patients even after years treatment. sustained presence, heterogeneity and evolvability LSCs are imperative for disease progression well recurrence during treatment-free remission (TFR). However, dynamic changes among LSC sub-populations upon therapy impede their measurement targeting....
In the adult murine brain, neural stem cells (NSCs) can be found in two main niches: dentate gyrus (DG) and subventricular zone (SVZ). DG, NSCs produce intermediate progenitors (IPs) that differentiate into excitatory neurons, while SVZ migrate to olfactory bulb (OB), where they mainly inhibitory interneurons. Neurogenesis, process of generating new persists throughout life but decreases dramatically with aging, concomitantly increased inflammation. Although many cell types, including...
Immunophenotypic characterization and molecular analysis have long been used to delineate heterogeneity define distinct cell populations. FACS is inherently a single-cell assay, however prior analysis, the target cells are often prospectively isolated in bulk, thereby losing resolution. Single-cell gene expression provides means understand differences between individual heterogeneous In bulk an overrepresentation of type results biases occlusions signals from rare with biological importance....
Abstract The advent of tyrosine kinase inhibitors (TKIs) as treatment chronic myeloid leukemia (CML) is a paradigm in molecularly targeted cancer therapy. Nonetheless, TKI insensitive stem cells (LSCs) persist most patients even after years treatment. sustained presence, heterogeneity and evolvability LSCs are imperative for disease progression well recurrence during treatment-free remission (TFR). However, dynamic changes among LSC sub-populations upon therapy impede their measurement...
Abstract Rare hematopoietic stem cells make up an infrequent but critical population in the bone marrow (BM), maintaining and replenishing entire system. Importantly, despite sharing unique cell properties of multilineage differentiation self-renewal, individual HSCs are functionally heterogeneous, this heterogeneity increases during aging. While young mice qualitatively more similar, ageing is marked by increased size HSC pool substantial functional variation HSCs. CD9 a surface marker that...