A5087878927- Asthma and respiratory diseases
- Cytokine Signaling Pathways and Interactions
- Protein Tyrosine Phosphatases
- Neonatal Respiratory Health Research
- NF-κB Signaling Pathways
- Genomics, phytochemicals, and oxidative stress
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Ubiquitin and proteasome pathways
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Respiratory Support and Mechanisms
- Drug Transport and Resistance Mechanisms
- Medical Imaging and Pathology Studies
- Hormonal Regulation and Hypertension
- RNA modifications and cancer
- Helicobacter pylori-related gastroenterology studies
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- Phosphodiesterase function and regulation
- Histone Deacetylase Inhibitors Research
- IL-33, ST2, and ILC Pathways
- Synthesis and Catalytic Reactions
- Inhalation and Respiratory Drug Delivery
- Cancer Cells and Metastasis
- Inflammatory mediators and NSAID effects
Lund University
2020-2024
Gene Therapy Laboratory
2021
University of Groningen
2017-2019
University Medical Center Groningen
2019
The University of Sydney
2014-2016
Migration Institute of Australia
2015
The mast cell-specific metalloprotease CPA3 has been given important roles in lung tissue homeostasis and disease pathogenesis. However, the dynamics spatial distribution of cell expression diseases remain unknown. Using a histology-based approach for quantitative decoding mRNA protein single cell, this study investigates across cells residing lungs from control subjects patients with severe chronic obstructive pulmonary (COPD) or idiopathic fibrosis (IPF). Mast COPD had an anatomically...
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Cytokines drive many inflammatory diseases, including asthma. Understanding the molecular mechanisms responsible for cytokine secretion will allow us to develop novel strategies repress inflammation in future. Harnessing power of endogenous anti-inflammatory proteins is one such strategy. In this study, we investigate p38 MAPK-mediated regulatory interaction two proteins, mitogen-activated protein kinase phosphatase 1 (MKP-1) and tristetraprolin (TTP), context asthmatic inflammation. Using...
Airway basal cells are crucial for regeneration of the human lung airway epithelium and believed to be important contributors chronic obstructive pulmonary disease (COPD) other disorders. To reveal how contribute discover novel therapeutic targets, these need further characterized. In this study, we optimized a flow cytometry-based cell sorting protocol primary dependent on size NGFR (nerve-growth factor receptor) expression. The population was found molecularly functionally heterogeneous,...
Exaggerated cytokine secretion drives pathogenesis of a number chronic inflammatory diseases, including asthma. Anti-inflammatory pharmacotherapies, corticosteroids, are front-line therapies and although they have proven clinical utility, the molecular mechanisms responsible for their actions not fully understood. The corticosteroid-inducible gene, mitogen-activated protein kinase (MAPK) phosphatase 1 (MKP-1, DUSP1) has emerged as key molecule repressive effects steroids. MKP-1 is known to...
IL-17A plays an important role in respiratory disease and is a known regulator of pulmonary inflammation immunity. Recent studies have linked with exacerbation asthma COPD. We shown that the enzyme cyclooxygenase-2 (COX-2) its prostanoid products, prostaglandin E2 (PGE2 ) particular, are key contributors vitro models infectious exacerbation; however, impact was not known.We address this herein show induces robust sustained upregulation COX-2 protein PGE2 secretion from airway smooth muscle...
Airway inflammation and remodeling are characteristic features of asthma, with both contributing to airway hyperresponsiveness (AHR) lung function limitation. smooth muscle (ASM) accumulation extracellular matrix deposition remodeling, which may contribute persistent AHR. Laminins containing the α2-chain characteristics ASM in vitro AHR animal models asthma. The role other laminin chains, including α4 α5 leukocyte migration diseases, is currently unknown. aim current study was investigate...
Mitogen activated protein kinase phosphatase-1 (MKP-1) has emerged as an important mediating breast cancer oncogenesis and chemoresistance to chemotherapies, especially proteasome inhibitors. In this in vitro study, we utilized the epithelial cell lines MCF-7 MDA-MB-231, comparison MCF-10A control cells, examine impact of MKP-1 on growth repression by We confirm that inhibitors MG-132 bortezomib induce upregulation show one ways which increases addition inhibition ubiquitin-proteasome...
Cell-based therapies hold great promise in re-establishing organ function for many diseases, including untreatable lung diseases such as idiopathic pulmonary fibrosis (IPF). However, hurdles still remain, part due to our lack of knowledge about the disease-driving mechanisms that may affect cellular niche and thereby possibly hinder any transplanted cells by imposing disease phenotype onto newly generated progeny. Recent findings have demonstrated increased ciliation from IPF patients, but...
Combination treatment has proven effective for patients with acute promyelocytic leukemia, exemplifying the importance of therapy targeting multiple components oncogenic regulation a successful outcome. However, recent studies have shown that mutational complexity myeloid leukemia (AML) precludes translation molecular into clinical success. Here, as complement to genetic profiling, we used unbiased, combinatorial in vitro drug screening identify pathways drive AML and develop personalized...
Abstract Purpose The aim of this study was to set up reliable and reproducible culture conditions for 3D tumoroids derived from non-small cell lung cancer (NSCLC) lines enable greater opportunity successful cultivation patient-derived samples. Methods Four NSCLC lines, two adenocarcinomas (A549, NCI-H1975) squamous carcinomas (HCC-95, HCC-1588), were first cultured in traditional 2D settings. Their expected expression profiles concerning TTF-1, CK7, CK5, p40 status confirmed by...
From a previously performed proteomics screen, GPP130, or Golgi phosphoprotein of 130 kDa, was identified as potential substrate the proprotein convertase 7 (PC7; PCSK7). GPP130 is type-II transmembrane protein with luminal domain containing endosomal and Golgi-retrieval determinants, enabling unique trafficking route. Most previous work on relates to its binding retrograde Shiga toxin. Recently, reported be implicated in cell cycle progression proliferation head neck cancer cells. This led...
Summary The maternal body helps in providing nutrients and degrading toxic metabolites instead of the fetal body; disruptions these mechanisms affect normal development. Sterol 27-hydroxylase ( Cyp27a1 ) is involved alternative pathway bile acid synthesis, which enhanced during pregnancy. However, its role development remains unclear. Here, we demonstrate that activity essential for progression pregnancy organ formation. Depletion reduced rate litter size. Newborn mice died respiratory...
Aging attenuates the ability of lung to regenerate lost or damaged tissue and could be responsible for decline in airway stem cell function. In addition, other naturally occurring aging phenomena such as cellular senescence epigenetic alterations can impact progression many chronic diseases (such COPD Cancer). Still, how affects human on a molecular level remains unknown. Therefore, it is important delineate changes that occur within "healthy" order understand provide context development...
Aging attenuates the ability of lung to regenerate lost or damaged tissue and could be responsible for decline in airway stem cell function. In addition, other naturally occurring aging phenomena such as cellular senescence epigenetic alterations can impact progression many chronic diseases (such COPD Cancer). Still, how affects human on a molecular level remains unknown. Therefore, it is important delineate changes that occur within "healthy" understand provide context development disease....