A5102992777- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- PI3K/AKT/mTOR signaling in cancer
- Metabolism, Diabetes, and Cancer
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Protein Degradation and Inhibitors
- Extracellular vesicles in disease
- Sarcoma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Cell Adhesion Molecules Research
- Virus-based gene therapy research
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
Jiangyin People's Hospital
2016-2022
Xuzhou Medical College
2022
Southeast University
2016-2021
HBO1 (KAT7 or MYST2) is a histone acetyltransferase that acetylates H3 and H4 histones. Methods: expression was tested in human OS tissues cells. Genetic strategies, including shRNA, CRISPR/Cas9 overexpression constructs, were applied to exogenously alter The inhibitor WM-3835 utilized block activation. Results:HBO1 mRNA protein significantly elevated In established (MG63/U2OS lines) primary cells, shRNA-mediated silencing CRISPR/Cas9-induced knockout able potently inhibit cell viability,...
Sustained activation of multiple receptor tyrosine kinases (RTKs) simultaneously is vital for tumorigenesis and progression osteosarcoma (OS). Gαi proteins recruitment to various RTKs mediates downstream oncogenic signaling activation. The expression, functions underlying mechanisms Gαi3 in human OS were examined. Expression robustly elevated tissues correlated with a poor overall survival. In patient-derived primary cells immortalized lines (MG63 U2OS), depletion, by shRNA CRISPR/Cas9...
Malignant osteosarcoma (OS) is still a deadly disease for many affected patients. The search the novel anti-OS agent extremely urgent and important. Our previous study has proposed that salinomycin agent. Here we characterized DNA-dependent protein kinase catalytic subunit (DNA-PKcs) as primary resistance factor in OS cells. DNA-PKcs inhibitors (NU7026, NU7441 LY294002) or shRNA knockdown dramatically potentiated salinomycin-induced death apoptosis of cells (U2OS MG-63 lines). Further,...
Mammalian target of rapamycin (mTOR) complex 1 (mTORC1) and mTORC2 are frequently dysregulated in human colon cancers. In the present study, we evaluated potential anti-colon cancer cell activity by a novel mTORC1/2 dual inhibitor WYE-354. We showed that WYE-354 was anti-survival anti-proliferative when adding to primary (patient-derived) established (HCT-116, HT-29, Caco-2, LoVo, DLD-1 lines) cells. addition, treatment activated caspase-dependent apoptosis Mechanistically, blocked mTORC1...
POLRMT (RNA polymerase mitochondrial) is essential for transcription of mitochondrial genome encoding components oxidative phosphorylation process. The current study tested expression and its potential function in osteosarcoma (OS). Cancer Genome Atlas (TCGA) cohorts Gene Expression Profiling Interactive Analysis (GEPIA) database both show that transcripts are elevated OS tissues. In addition, mRNA protein levels were upregulated local tissues as well established primary human cells....
Sphingosine kinase 1 (SphK1) is a potential therapeutic target for human osteosarcoma (OS).SphK1-targeting microRNAs (miRNAs) could have important value OS.We discovered that micorRNA-3677 (miR-3677) SphK1-targeting miRNA, inhibiting OS cell progression.The results of RNA-Pull down assay confirmed direct binding between biotinylated-miR-3677 and SphK1 mRNA in primary cells.In established cells forced overexpression miR-3677, by lentiviral construct, decreased 3'-UTR (untranslated region)...
Osteosarcoma (OS) is a common primary bone malignancy. We here investigated the potential activity of PF-06409577, novel, potent, and direct activator AMP-activated protein kinase (AMPK), against human OS cells. In established (U2OS, MG-63, SaOs-2 lines) cells, PF-06409577 inhibited cell viability proliferation, while inducing apoptosis cycle arrest. induced AMPK activation, mTORC1 inhibition, autophagy induction, downregulation multiple receptor tyrosine inOS inactivation by α 1 shRNA,...
// Zheng-Wei Li 1, * , Yun-Rong Zhu 2, Xiao-Zhong Zhou 3, 4, Bao-Biao Zhuo 1 Xiao-Dong Wang The Center of Diagnosis and Treatment for Children's Bone Diseases, Hospital Affiliated to Soochow University, Suzhou, China 2 Department Orthopedics, Jiangyin Medical College Southeast City, 3 Second 4 First People's SuQian, Co-first authors Correspondence to: Wang, email: xiaodongwangsz@163.com Keywords: microRNA-135b, Ppm1e, AMPK, osteoblastoma, cell proliferation Received: January 20, 2017...