A5029184531- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Toxin Mechanisms and Immunotoxins
- vaccines and immunoinformatics approaches
- Parasites and Host Interactions
- Hepatitis C virus research
- Synthesis and Biological Evaluation
- Insect and Pesticide Research
- Blood Coagulation and Thrombosis Mechanisms
- Viral gastroenteritis research and epidemiology
- Cervical Cancer and HPV Research
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- 3D Printing in Biomedical Research
- Parasitic Infections and Diagnostics
- RNA and protein synthesis mechanisms
- bioluminescence and chemiluminescence research
- Biochemical and Molecular Research
- Insect Pest Control Strategies
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Cells and Metastasis
- Toxoplasma gondii Research Studies
Pasteur Institute of Iran
2015-2025
Iran University of Medical Sciences
2022
National Institute of Genetic Engineering and Biotechnology
2010
Novel vaccination approaches are needed to prevent leishmaniasis. Live attenuated vaccines the gold standard for protection against intracellular pathogens such as Leishmania and there have been new developments in this field. The nonpathogenic humans lizard protozoan parasite, (L) tarentolae, has used effectively a vaccine platform visceral leishmaniasis experimental animal models. Correspondingly, pre-exposure sand fly saliva or immunization with salivary protein shown protect mice...
Leishmaniasis caused by protozoan parasites of the genus Leishmania. Intracellular infections treatment such as leishmaniasis is frequently hampered limited access drugs to infected cells. Moreover, most current are confined some toxic compounds, and there increasing incidences development drug resistance. Hence, production a new antileishmanial compound crucial. Paromomycin sulphate (PM) promising drug. One strategy improve effectiveness use appropriate delivery systems. Solid lipid...
The crucial oncogenic role of cancer stem cells (CSCs) in tumor maintenance, progression, drug resistance, and relapse has been clarified different cancers, particularly colorectal (CRC). current study was conducted to evaluate the co-expression pattern clinical significance epithelial cell adhesion molecules (EpCAM) activated leukocyte (CD166 or ALCAM) CRC patients.This carried out on 458 paraffin-embedded specimens by immunohistochemistry tissue microarray (TMA) slides.Elevated expression...
Visceral leishmaniasis (VL) is a vector-borne disease affecting humans and domestic animals that constitutes serious public health problem in many countries. Although antigens have been examined so far as protein- or DNA-based vaccines, none of them conferred complete long-term protection. The use the lizard non-pathogenic to Leishmania (L.) tarentolae species live vaccine vector deliver specific recent approach needs be explored further. In this study, we evaluated effectiveness vaccination...
Proliferation of Leishmania (L.) parasites depends on polyamine availability, which can be generated by the L-arginine catabolism and enzymatic activity arginase (ARG) mammalian hosts. In present study, we characterized compared (arg) genes from pathogenic L. major tropica non-pathogenic tarentolae. We quantified level ARG in promastigotes macrophages infected with tarentolae amastigotes. The ARG's amino acid sequences demonstrated virtually 98.6% 88% identities reference Friedlin ARG....
Background The vector-borne disease leishmaniasis is transmitted to humans by infected female sand flies, which transmits Leishmania parasites together with saliva during blood feeding. In Iran, cutaneous (CL) caused (L.) major and L. tropica, their main vectors are Phlebotomus (Ph.) papatasi Ph. sergenti, respectively. Previous studies have demonstrated that mice immunized the salivary gland homogenate (SGH) of or subjected bites from uninfected flies protected against infection. Methods...
Canine Visceral Leishmaniasis (CVL) is a major veterinary and public health problem caused by Leishmania infantum (L. infantum) in many endemic countries. It severe chronic disease with generalized parasite spread to the reticuloendothelial system, such as spleen, liver bone marrow often fatal when left untreated. Control of VL dogs would dramatically decrease infection pressure L. for humans, since are main domestic reservoir. In past decade, various subunits DNA antigens have been...
Leishmaniasis is a vector-borne disease caused by the protozoan parasite of Leishmania genus and complex affecting mostly tropical regions world. Unfortunately, despite extensive effort made, there no vaccine available for human use. Undoubtedly, comprehensive understanding host-vector-parasite interaction substantial developing an effective prophylactic vaccine. Recently role sandfly saliva on progression has been uncovered which can make contribution in design. In this review we try to...
Hypervariability of HCV proteins is an important obstacle to design efficient vaccine for infection. Multi-epitope vaccines containing conserved epitopes the virus could be a promising approach protection against HCV.Cellular and humoral immune responses multi-epitope DNA peptide were evaluated in BALB/c mice.In this experimental study, DNA- peptide-based infection harboring immunodominant CD8+ T cell (HLA-A2 H2-Dd) from Core (132-142), NS3 (1073-1081) NS5B (2727-2735), Th CD4+ epitope...
Background There are several reports demonstrating the role of CD8 T cells against Leishmania species. Therefore peptide vaccine might represent an effective approach to control infection. We developed a rational polytope-DNA construct encoding immunogenic HLA-A2 restricted peptides and validated processing presentation encoded epitopes in preclinical mouse model humanized for MHC-class-I II. Methods Findings HLA-A*0201 from LPG-3, LmSTI-1, CPB CPC along with H-2Kd peptides, were lined-up...
Leishmaniasis is a serious global health problem affecting many people worldwide. While patients with leishmaniasis can be treated several agents, drug toxicicty and the emergence of resistant strains render available treatments ineffective in long run. Inhibitors mammalian target rapamycin (mTOR) have been demonstrated to exert anti-pathogen properties. In this study, we tested therapeutic efficacy mTOR inhibitors controlling infection Leishmania major. Rapamycin, GSK-2126458 KU-0063794...
Background: Cervical cancer, the third most prevalent cause of cancer in women worldwide, is associated with HPVs. The critical role E7 protein HPV-related malignancies has designated it as a strong contender for generating vaccines against HPV. Materials & methods: In this study, we developed novel live vaccine using recombinant Leishmania tarentolae expressing E7-green fluorescent (GFP) fusion protection mice HPV-associated tumors. order to transfect L. E7-GFP construct, pLEXSY-neo2 system...