A5101645747- Virus-based gene therapy research
- MicroRNA in disease regulation
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Circular RNAs in diseases
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- Polysaccharides and Plant Cell Walls
- interferon and immune responses
- Advanced Biosensing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Sulfur-Based Synthesis Techniques
- Hepatitis C virus research
- PI3K/AKT/mTOR signaling in cancer
- Electrospun Nanofibers in Biomedical Applications
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Immune Response and Inflammation
- Natural product bioactivities and synthesis
- Psoriasis: Treatment and Pathogenesis
- Mesenchymal stem cell research
- Adenosine and Purinergic Signaling
- NF-κB Signaling Pathways
Second Hospital of Hebei Medical University
2023
Luoyang Institute of Science and Technology
2022
Henan University Huaihe Hospital and Huaihe Clinical Institute
2017-2021
Dalian Medical University
2018-2019
Ono Pharmaceutical (United States)
2016-2019
Chongqing Medical University
2018-2019
Second Affiliated Hospital of Chongqing Medical University
2018-2019
Liaocheng People's Hospital
2018
Taishan Medical University
2018
Research & Development Institute
2017
Poria cocos polysaccharide (PCP) is the major active ingredients of P. and possesses various pharmacological effects, including anti-oxidative anti-apoptosis effects activity against cancer. This study investigated immunomodulatory mechanism by which PCP acts on RAW 264.7 macrophages LLC tumors in mice.The concentrations nitric oxide, Th1, Th2, Th17 cytokines were examined Griess reaction using a bead-based cytokine assessment kit. qRT-PCR western blotting used to investigate relevant...
Recombinant Newcastle disease virus (rNDV) have shown oncolytic therapeutic efficacy in preclinical studies and are currently clinical trials. In this study, we evaluated the possibility to enhance cancer potential of NDV by means inserting both interleukin-2 (IL-2) tumor necrosis factor-related apoptosis inducing ligand (TRAIL) delivered rNDV. We demonstrated that rNDV expressing TRAIL (rNDV-TRAIL) or human IL-2 (rNDV-IL-2-TRAIL) significantly enhanced inherent anti-neoplastic apoptosis....
Bruton's tyrosine kinase (Btk) is expressed in a variety of immune cells and previous work has demonstrated that blocking Btk promising strategy for treating autoimmune diseases. Herein, we utilized tool inhibitor, M7583, to determine the therapeutic efficacy inhibition two mouse lupus models driven by TLR7 activation type I interferon. In BXSB-Yaa mice, reduced autoantibodies, nephritis, mortality. pristane-induced DBA/1 model, suppressed arthritis, but autoantibodies IFN gene signature...
Recombinant Newcastle Disease Virus (rNDV) has shown oncolytic therapeutic effect in preclinical studies. Previous data indicate that rNDV carrying IL2 promise cancer therapy. Due to the significant side effects of IL2, IL15 been introduced into A number studies have suggested efficiently enhances activities CTL and NK cells inhibits tumor recurrence metastasis. Furthermore, is less toxic than IL2. Therefore, we hypothesize a recombinant NDV expressing would be promising agent for treatment...
Purpose: Hepatitis C virus (HCV) infection is a major cause of liver disease.Several miRNAs have been found to be associated with HCV infection.This study aimed investigate the functional roles and possible molecular mechanisms miR-215 in replication. Materials Methods:The expression levels TRIM22 were detected by quantitative real-time PCR (qRT-PCR) western blot analysis Con1b subgenomic genotype 1b replicon cells (Con1b cells) JFH1 full genome infecting Huh7.5.1 (Huh7.5.1 cells).HCV RNA...
Rheumatoid arthritis (RA) is an autoimmune disease, primarily manifesting as inflammatory arthritis. It associated with chronic inflammation of the synovial joints, mostly in hands and feet, well systemic extra-articular inflammation. The chimeric anti-interleukin (IL)-17 full-length monoclonal antibody (CMa17Aab) targets IL-17A, which important cytokine pathogenesis RA other disorders. In this study, we investigated whether CMa17Aab exerts therapeutic effects a mouse model type II...
Abstract: Circular RNAs act as vital regulators in diverse diseases. However, the investigation of circular sepsis-engendered acute kidney injury remains dismal. We aimed to explore effects RNA protein kinase C iota (circ-PRKCI) lipopolysaccharide (LPS)-mediated HK2 cell injury. Sepsis vitro model was established by LPS treatment. Quantitative real-time polymerase chain reaction assay conducted for determining levels circ-PRKCI, microRNA-106b-5p (miR-106b-5p), and growth factor receptor...
ABSTRACT Sepsis‐induced skeletal muscle wasting may lead to various severe clinical consequences. Understanding molecular mechanisms of the regulation loss mass in septic patients remains a significant challenge. The current study was conducted establish mouse models explore relationship between microRNA (miR)‐351 and transcription element apical (TEA) domain factor (Tead)‐4 gene investigate its effects on through mediating Hippo signaling pathway mice with acute sepsis. A total 60 were...
Intravenous transplantation has been regarded as a most safe method in stem cell therapies. There is evidence showing the homing of bone marrow cells (BMSCs) into injured sites, and thus these can be used treatment acute myocardial infarction (MI). This study aimed to investigate effect intravenous epicardial transplantion BMSCs on size rabbit model.A total 60 New Zealand rabbits were randomly divided three groups: control group, epicardium group (group I) ear vein II). The collected from...
Taccalonolide A has been reported to have anti-tumour efficiency. However, the underlying mechanism for taccalonolides therapy of hepatocellular carcinoma (HCC) is still obscure. Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. Apoptosis determined flow cytometry. Protein expression B lymphoma (Bcl-2), Bcl-2 associated X (Bax), sonic hedgehog (Shh), Smoothened (Smo) and Gli family zinc finger 1 (Gli1) analyzed western blot. The Shh, Smo Gli1 mRNA using quantitative...
Objective To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method.Methods A was developed conjugation of the antigen gp41 and gp36 200nm super paramagnetic particles carbodiimide (EDC) coating sp41 nitrocellulose membrane.Then evaluated with serials experiments.Results The qualified examination national reference panel anti-HIV colloidal gold diagnostic kit.The sensitivity 100% tested 20 positive sera,the specificity 98.5% 600 negative sera,respectively.The...
Introduction Emerging evidence identifies that microRNAs (miRNAs) are associated with hepatitis B virus (HBV) infection. In the current study, we mainly focus on functions and underlying mechanisms of miR-212-3p in HBV replication hepatocellular carcinoma (HCC). Material methods The levels miR-212-3p, nuclear factor I A (NFIA) DNA copies were measured by qRT-PCR. level core particle-associated DNA, productions surface antigen (HBsAg) e-antigen (HBeAg), expression NFIA detected via southern...
Abstract Post-translational palmitoylation of intracellular proteins is mediated by protein palmitoyltransferases (PAT) belonging to the DHHC family, which share a common catalytic Asp-His-His-Cys (DHHC) motif. According genome-wide siRNA screen scoring for growth suppression in over 50 non-small cell lung cancer (NSCLC) lines, family members are strikingly over-represented. Among tested palmitoyltransferases, knockdown DHHC5 showed inhibition many lines initial screen. To further...