Abstract Astragalus polysaccharides (APS), which is widely used as a remedy to promote immunity of breast cancer patients, can enhance immune responses and exert anti-tumor effects. In this study, we investigated the effects mechanisms APS on macrophage RAW 264.7 EAC tumor-bearing mice. Griess reaction ELISA assays revealed that concentrations nitric oxide, TNF-α, IL-1β IL-6 were increased by APS. However, effect was diminished in presence TAK-242 (TLR4 inhibitor) or ST-2825(MyD88...

Poria cocos polysaccharide (PCP) is the major active ingredients of P. and possesses various pharmacological effects, including anti-oxidative anti-apoptosis effects activity against cancer. This study investigated immunomodulatory mechanism by which PCP acts on RAW 264.7 macrophages LLC tumors in mice.The concentrations nitric oxide, Th1, Th2, Th17 cytokines were examined Griess reaction using a bead-based cytokine assessment kit. qRT-PCR western blotting used to investigate relevant...

Retinoblastoma (RB) is a malignant intraocular neoplasm that occurs in children. Diagnosis and therapy are frequently delayed, often leading to metastasis, which necessitates effective imaging treatment. In recent years, the use of nanoplatforms allowing both targeted treatment has attracted much attention. Herein, we report novel nanoplatform folate-receptor (FR) laser-activatable liposome termed FA-DOX-ICG-PFP@Lip, loaded with doxorubicin (DOX)/indocyanine green (ICG) liquid...

AbstractThe aim of this study was to determine whether polysaccharopeptide (PSP) and Astragalus polysaccharides (APS) can be combined together as a new complex prescription (PSP + APS) for aiding adriamycin (AMD) chemotherapy. Ehrlich's ascites carcinoma (EAC) used establish solid tumor model in Kunming mice. Immunocytochemical immunohistochemical analysis were employed detect the immunoregulatory anti-tumor effects EAC bearing mice after 30 days administration with PSP APS. APS could...

Background Upper respiratory tract infection (URTI) is a major reason for hospitalization in childhood. More than 80% of URTIs are viral. Etiological diagnosis important to make correct clinical decisions on treatment methods. However, data viral spectrum very limited Shanghai children. Methods Nasopharyngeal swabs were collected from group 164 children aged below 3 years who hospitalized due acute May 2009 July 2010 Shanghai. A VRDAL multiplex PCR 10 common viruses was performed specimens...

A novel gold nanoparticle (Do-AuNP) was successfully synthesized from water extracts of traditional Chinese medicine Dendrobium officinale (DO) without using any extra chemicals regents. The physicochemical properties Do-AuNPs were analyzed by transmission electron microscopy, dynamic light scattering, energy-dispersive X-ray spectroscopy, Fourier transform infrared and atomic force microscopy. amount DO extract on the AuNPs about 13%. In order to evaluate anti-tumor efficiency biosafety,...

Breast cancer is the most common among women worldwide. Discomfort and fatigue are usually arisen from anticancer therapy such as surgery, radiotherapy, chemotherapy, hormonal therapy, or combination because of suppressed immunological functions. Yunzhi (Coriolus versicolor) can modulate various functions in vitro, vivo, human clinical trials. Danshen (Salvia miltiorrhiza) has been shown to benefit circulatory system by its vasodilating anti-dementia activity. The purpose this trial was...

Polysaccharopeptide (PSP), isolated from Coriolus versicolor COV-1 strain, is a protein-bound polysaccharide widely used as immunoadjuvant for cancer immunotherapy. Although the immunomodulatory activity of PSP has been well established, precise molecule mechanisms its biological have yet to be fully elucidated.In present study, we first investigated in peritoneal macrophages C57BL/10J (TLR4(+/+)) and C57BL/10ScCr (TLR4(-/-)) mice carrying defective toll-like receptor-4 (TLR4) gene then...

Lung cancer is the leading cause of cancer‑associated mortality worldwide. The aim present study was to identify differentially expressed genes (DEGs) and enriched pathways in lung by bioinformatics analysis, provide potential targets for diagnosis treatment. Valid microarray data 31 pairs tissues matched normal samples (GSE19804) were obtained from Gene Expression Omnibus database. Significance analysis gene expression profile used DEGs between tissues, a total 1,970 DEGs, which...

Alpha-fetoprotein (AFP) is the most important diagnostic and prognostic index of hepatocellular carcinoma (HCC). AFP-positive HCC can be easily diagnosed based on serum AFP level typical imaging features, but a number patients are negative (AFP < 20 ng/mL) for AFP. Therefore, it necessary to develop novel biomarkers AFP-negative HCC. RNA data from TCGA differential expression lncRNAs, miRNAs, mRNAs were downloaded analyze patterns between tissues normal tissues. A lncRNA-miRNA-mRNA ceRNA...

Gold nanoparticles (AuNPs) were successfully fabricated by Pholiota adiposa polysaccharide (PAP-1a) without employing any other chemicals. The physical and chemical properties of PAP-AuNPs determined using transmission electron microscopy (TEM), dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDXR), Fourier-transform infrared (FT-IR), atomic force (AFM). In an attempt to analyze the immune regulation, antitumor effect, biological safety, production NO TNF-α, IL-12p70,...

Context: Polysaccharopeptide (PSP), isolated from the Coriolus versicolor COV-1 strain, has been widely used as an immunoadjuvant for cancer immunotherapy.Objective: The present study was undertaken to examine role of PSP on TLR4-TIRAP/MAL-MyD88 signaling pathway in peripheral blood mononuclear cells (PBMCs) breast patients.Methods: For blockade TLR4, were cultured with or without and anti-TLR4 24 h, then mRNA proteins (IL-12, IL-6, TNF-α) levels each group detected by Q-PCR ELISA....