A5064244051- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- Suicide and Self-Harm Studies
- Epigenetics and DNA Methylation
- Pneumonia and Respiratory Infections
- Antibiotic Use and Resistance
- Mental Health Treatment and Access
- Neurotransmitter Receptor Influence on Behavior
- Employment and Welfare Studies
- Antibiotics Pharmacokinetics and Efficacy
- Neurogenetic and Muscular Disorders Research
- Substance Abuse Treatment and Outcomes
- Amyotrophic Lateral Sclerosis Research
- Parkinson's Disease Mechanisms and Treatments
- Bipolar Disorder and Treatment
- Prenatal Substance Exposure Effects
- Ion channel regulation and function
- Personality Disorders and Psychopathology
- Biosimilars and Bioanalytical Methods
- Biomedical Ethics and Regulation
- GABA and Rice Research
- Microbial Natural Products and Biosynthesis
- Cell Image Analysis Techniques
Institute of Biomedical Sciences, Academia Sinica
1997-2021
Johns Hopkins University
2020-2021
Johns Hopkins Medicine
2021
China Medical University
2020
University of California, Santa Cruz
2013-2018
Monash University
2017
The Alfred Hospital
2017
Academia Sinica
1999-2009
Takming University of Science and Technology
2002
National Taiwan University
2002
Importance Whether β-lactam antibiotics administered by continuous compared with intermittent infusion reduces the risk of death in patients sepsis is uncertain. Objective To evaluate whether vs a antibiotic (piperacillin-tazobactam or meropenem) results decreased all-cause mortality at 90 days critically ill sepsis. Design, Setting, and Participants An international, open-label, randomized clinical trial conducted 104 intensive care units (ICUs) Australia, Belgium, France, Malaysia, New...
The impact of media reporting suicides entertainment celebrities may affect suicide rates due to an imitation effect. We investigated the on a male television celebrity.All during 2003-2005 in Taiwan (n = 10,945) were included this study. A Poisson time series autoregression analysis was conducted examine whether there increase 4-week period after extensive celebrity suicide.After controlling for seasonal variation, calendar year, temperature, humidity and unemployment rate, marked number...
Two-component signal transduction systems (TCS) are used by bacteria to sense and respond their environment. TCS typically composed of a sensor histidine kinase (HK) response regulator (RR). The Vibrio cholerae genome encodes 52 RR, but the role these RRs in V. pathogenesis is largely unknown. To identify that control colonization, in-frame deletions each RR were generated resulting mutants analyzed using an infant mouse intestine colonization assay. We found 12 involved intestinal...
ABSTRACT Two-component systems play important roles in the physiology of many bacterial pathogens. Vibrio cholerae 's CarRS two-component regulatory system negatively regulates expression vps ( polysaccharide) genes and biofilm formation. In this study, we report that CarR confers polymyxin B resistance by positively regulating almEFG genes, whose products are required for glycine diglycine modification lipid A. We determined directly binds to region operon. Similarly a carR mutant, strains...
To date, most antibiotics have primarily been developed to target bacteria in the planktonic state. However, biofilm formation allows develop tolerance and provides a mechanism evade innate immune systems. Therefore, there is significant need identify small molecules prevent and, more importantly, disperse or eradicate preattached biofilms, which are major source of bacterial persistence nosocomial infections. We now present modular high-throughput 384-well image-based screening platform...
Neurodegenerative diseases are challenging for systems biology because of the lack reliable animal models or patient samples at early disease stages. Induced pluripotent stem cells (iPSCs) could address these challenges. We investigated DNA, RNA, epigenetics, and proteins in iPSC-derived motor neurons from patients with ALS carrying hexanucleotide expansions
Two-component signal transduction systems (TCSs), typically composed of a sensor histidine kinase (HK) and response regulator (RR), are the primary mechanism by which pathogenic bacteria sense respond to extracellular signals. The bacterium Vibrio cholerae is no exception harbors 52 RR genes. Using in-frame deletion mutants each gene, we performed systematic analysis their role in V. biofilm formation. We determined that 7 RRs impacted expression an essential gene found recently...
Biofilms are a ubiquitous feature of microbial community structure in both natural and host environments; they enhance transmission infectivity pathogens provide protection from human defense mechanisms antibiotics. However, few products known that impact biofilm formation or persistence for either environmental pathogenic bacteria. Using the combination novel library fish microbiome an image-based screen inhibition, we describe identification taurine-conjugated bile acids as inhibitors...
A bstract : Background: Nonselective opioid antagonists reduce alcohol consumption under various experimental situations, and several association studies have examined possible roles of receptor mu ( OPRM ), delta OPRD kappa OPRK ) genes in the development dependence. Methods: We 20 single nucleotide polymorphisms (SNPs) across , 158 alcohol‐dependent subjects 149 controls. Differences allele frequency genotype distribution between case controls, as well deviation from Hardy‐Weinberg...
Vibrio cholerae biofilms contain exopolysaccharide and three matrix proteins RbmA, RbmC Bap1. While much is known about regulation, little the mechanisms by which protein components of are regulated. VrrA a conserved, 140-nt sRNA V. cholerae, whose expression controlled sigma factor σE. In this study, we demonstrate that negatively regulates rbmC translation pairing to 5′ untranslated region transcript regulation not stringently dependent on RNA chaperone Hfq. These results point as...
Abstract Background We sought to establish a nonhuman primate model of vaginal Lactobacillus colonization suitable for evaluating live microbial microbicide candidates. Methods Vaginal and rectal microflora in Chinese rhesus macaques ( Macaca mulatta ) were analyzed, with cultivable bacteria identified by 16S rRNA gene sequencing. Live lactobacilli intravaginally administered evaluate bacterial colonization. Results harbored abundant , johnsonii as the predominant species. Like humans, most...
Biofilms play an important role in the Vibrio cholerae life cycle, contributing to both environmental survival and transmission a human host. Identifying key regulators of V. biofilm formation is necessary fully understand how this growth mode modulated response various signals encountered environment In study, we characterized RRs that function as coactivators RpoN regulating identified new components regulatory circuitry.
Abstract Bacterial biofilms pose a significant challenge in clinical environments due to their inherent lack of susceptibility antibiotic treatment. It is widely recognized that most pathogenic bacterial strains the setting persist biofilm state, and are root cause many recrudescent infections. The discovery development compounds capable either inhibiting formation or initiating dispersal might provide new therapeutic avenues for reducing number hospital‐acquired, biofilm‐mediated We detail...
Bacterial biofilms are estimated to be associated with over 65 percent of all nosocomial infections. However, no therapeutics have been approved by the FDA which directly mediate biofilm formation or persistence. Herein we report oxazine as a highly potent inhibitor, disperser and in presence appropriate antibiotic eradicator V. cholerae biofilms.
This study established the psychometric properties of Chinese version Suicide Intent Scale (SIS) in a clinic‐ and community‐based sample 36 patients 592 respondents, respectively. Results showed that SIS demonstrated good inter‐rater test‐retest reliability. Factor analysis generated three factors (Precautions, Planning, Seriousness) explaining 92.9% total variance with high internal consistency. It was moderately correlated depressive symptoms. suggest is reliable valid instrument for use...
Neuroblastoma is a solid tumor occurring usually in children less than 5 years old. It has been difficult to distinguish neuroblastoma from other childhood tumors through morphological diagnosis. Urine homovanillic acid (HVA), which metabolite of dopamine, proposed as diagnostic index. Although increased levels serotonin metabolite, 5-hydroxyindole-3-acetic (HIAA), have also observed urine samples the patients, they were largely attributed dietary amines. By using an HPLC system with...
Background Suicide rates are higher in men than women most countries, although the gender ratios vary markedly worldwide. We investigated long-term trends suicide and male-to-female relation to age, method economic factors Taiwan during Japanese colonial (1905–1940) postwar (1959–2012) periods. Methods data were from Statistical Reports of Governor’s Office (1905–1940), Vital Statistics (1959–1970) cause-of-death mortality files (1971–2012). Annual age-standardised...