A5101731262- Bone Metabolism and Diseases
- TGF-β signaling in diseases
- Bone health and treatments
- Extracellular vesicles in disease
- Sirtuins and Resveratrol in Medicine
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- CAR-T cell therapy research
- Kruppel-like factors research
- PI3K/AKT/mTOR signaling in cancer
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- NF-κB Signaling Pathways
- RNA modifications and cancer
- Estrogen and related hormone effects
- Nanoplatforms for cancer theranostics
- Peroxisome Proliferator-Activated Receptors
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
- Bone and Dental Protein Studies
- Epigenetics and DNA Methylation
Chonnam National University
2011-2022
Gwangju Institute of Science and Technology
2018-2022
Ministry of Food and Drug Safety
2022
Korea Photonics Technology Institute
2019
Abstract Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to II CRISPR–Cas9 for genome editing. Previous studies have revealed that has unique features distinct from Cas9, but the detailed mechanisms of target searching and cleavage by are still unclear. Here, we directly observe this entire process using single-molecule fluorescence assays study Acidaminococcus sp. (AsCas12a). We determine AsCas12a...
Macrophages (MΦs) have the capability to sense chemotactic cues and home tumors, therefore presenting a great approach engineer these cells deliver therapeutic agents treat diseases. However, current cell-based drug delivery systems usually use commercial cell lines that may elicit an immune response when injected into host animal. Furthermore, premature off-target release also remains enormous challenge. Here, we isolated differentiated MΦs from spleens of BALB/c mice developed...
Exosomes are nanosized extracellular vesicles secreted by various cell types, including those of the immune system, such as natural killer (NK) cells. They play a role in intercellular communication transporting signal molecules between Recent studies have reported that NK cell-derived exosomes (NK-exo) contain cytotoxic proteins-induced death. However, characteristics and potential functions NK-exo, especially for liver cancer poorly understood. In this study, we investigated anti-tumor...
Runx2 plays essential roles in bone formation and chondrocyte maturation. Akt promotes osteoblast differentiation induced by the morphogenetic proteins BMP2 enhances function transcriptional activity of Runx2. However, precise molecular mechanism underlying relationship between is not well understood. In this study, we examined role regulating function. We found that increases stability protein. level mRNA was affected Akt, did find any evidence for direct modification Akt. Instead, induces...
Exosomes are nanosized extracellular vesicles secreted by various cells, including natural killer (NK) and known for their low toxicity, high permeability, biocompatibility, strong targeting ability. NK cell-derived exosomes (NK-exos) contain cytotoxic proteins that enhance tumor-targeting efficiency, making them suitable treating solid tumors such as hepatocellular carcinoma (HCC). Despite potential in drug delivery, the mechanisms of drug-loaded NK-exos, particularly those loaded with...
Exosomes are released by various cells, including natural killer (NK) cells and transport signaling molecules for the intercellular communication. Hepatocellular carcinoma (HCC), also known as primary liver cancer, is often inoperable difficult to accurate diagnosis. Notably, prognosis underlying mechanisms of HCC not fully understood. Exosomes-derived NK (NK-exos) express unique cytotoxic proteins with a killing ability in tumors can easily penetrate tumor tissues improve their targeting...
Colorectal cancer remains one of the main causes cancer-related deaths worldwide. Although numerous nanomedicine formulations have been developed to tackle disease, their low selectivity still limits effective therapeutic outcomes. In this study, we isolated extracellular vesicles (EVs) from CT26 colorectal cells and 4T1 murine mammary carcinoma cells, loaded them with chemotherapeutic agent (doxorubicin, DOX). Then evaluated cellular uptake both in 2D monolayer 3D tumor spheroid setups...
Abstract Pseudoshikonin I (PSI), a novel biomaterial isolated from Lithospermi radix, has been recognized as an herbal medicine for the treatment of infectious and inflammatory diseases. Bone remodeling maintains balance through bone resorption (osteoclastogenesis) formation (osteoblastogenesis). is generally attributed to osteoblasts. However, effects PSI on are not well known. In this study, we found that ethanol extracts induced osteoblast differentiation by increasing expression...
Osterix belongs to the SP gene family and is a core transcription factor responsible for osteoblast differentiation bone formation. Activation of protein kinase A (PKA), serine/threonine kinase, essential controlling formation BMP-induced differentiation. However, relationship between PKA still unclear. In this report, we investigated precise role pathway in regulating during We found that increased level Osterix; phosphorylated Osterix, stability, enhanced transcriptional activity Osterix....
Bone remodeling and homeostasis are largely the result of coordinated action osteoblasts osteoclasts. Osteoblasts responsible for bone formation. The differentiation is regulated by transcription factors, Runx2 Osterix. Natural products plant origin still a major part traditional medicinal systems in Korea. root Lithospermum erythrorhizon Sieb. et Zucc. (LR), purple gromwell, an herbal medicine used inflammatory infectious diseases. LR anti-inflammatory exerts anticancer effects inducing...
Various cell therapy strategies, including chimeric antigen receptor-expressing T or natural killer (NK) cells and cell-mediated drug delivery, have been developed for tumor eradication. However, the efficiency of these strategies against solid tumors remains unclear. We hypothesized that real-time control visualization therapeutic cells, such as NK would improve their efficacy tumors. In this study, we engineered Sonazoid microbubble-conjugated (NK_Sona) demonstrated they were detectable by...
Peptidyl-prolyl isomerase 1 (Pin1) is the only enzyme known to catalyze isomerization of pSer/Thr-Pro peptide bond. Pin1 induces conformational change substrates and subsequently regulates diverse cellular processes. However, its role in osteoblast differentiation not well understood. Here we show that enhances differentiation. interacts affects protein stability transcriptional activity an important osteogenic factor Runx2. Our results indicate this regulation likely due suppression...
Estrogen receptor α (ER-α), which is involved in bone metabolism and breast cancer, has been shown to have transcriptional targets. Dlx3 essential for the skeletal development plays an important role osteoblast differentiation. Various osteogenic stimulators transcription factors can induce protein expression of Dlx3. However, regulatory function ER-α mediated process remains unknown. Therefore, we investigated regulation found that a positive regulator BMP2-induced We also interacts with...
Protein kinase A (PKA), a serine/threonine kinase, regulates bone formation, and enhances Bone morphogenetic protein (BMP)-induced osteoblast differentiation. However, the mechanisms of how PKA controls cellular response to BMP are not well known. We investigated effects modulating activity during BMP2-induced differentiation, found that function Dlx3. Dlx3 plays crucial roles in differentiation it is expressed most skeletal elements development. activation increases expression protein,...