A5036642838- Plant-Microbe Interactions and Immunity
- Phytochemicals and Antioxidant Activities
- Folate and B Vitamins Research
- Nutritional Studies and Diet
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Synthesis and Biological Evaluation
- Tendon Structure and Treatment
- Antioxidant Activity and Oxidative Stress
- Polyamine Metabolism and Applications
- Fatty Acid Research and Health
- Cancer therapeutics and mechanisms
- Plant pathogens and resistance mechanisms
- Plant Pathogens and Fungal Diseases
- DNA and Nucleic Acid Chemistry
- Adipokines, Inflammation, and Metabolic Diseases
- African Botany and Ecology Studies
- Porphyrin Metabolism and Disorders
- Trace Elements in Health
- Cancer-related Molecular Pathways
- Veterinary Equine Medical Research
- Vitamin C and Antioxidants Research
- Food composition and properties
- Natural Antidiabetic Agents Studies
- Cancer, Lipids, and Metabolism
University of Aberdeen
2010-2024
James Hutton Institute
2022
Incell Corporation (United States)
2008
Wye College
1994-2000
University of London
1994-2000
Hungarian Academy of Sciences
1994
M.G. Kholodny Institute of Botany
1994
The active oxygen species hydrogen peroxide (H2O2) was detected cytochemically by its reaction with cerium chloride to produce electron-dense deposits of perhydroxides. In uninoculated lettuce leaves, H2O2 typically present within the secondary thickened walls xylem vessels. Inoculation wild-type cells Pseudomonas syringae pv phaseolicola caused a rapid hypersensitive (HR) during which highly localized accumulation found in plant cell adjacent attached bacteria. Quantitative analysis...
Background Health-promoting polyunsaturated fatty acids (PUFA) are abundant in forages grazed by ruminants and vegetable fish oils used as dietary supplements, but only a small proportion of PUFA finds its way into meat milk, because biohydrogenation the rumen. Butyrivibrio fibrisolvens plays major role this activity. The aim study was to investigate mechanisms which affect growth B. , how metabolized metabolic response presence PUFA. Results Linoleic acid (LA; cis -9, -12-18:2) α-linolenic...
Abstract Peroxidase activity was characterized in lettuce (Lactuca sativaL.) leaf tissue. Changes the and distribution of enzyme were examined during development a nonhost hypersensitive reaction (HR) induced by Pseudomonas syringae (P. s.) pv phaseolicola response to anhrp mutant bacterium. Assays tissue extracts revealed pH optima 4.5, 6.0, 5.5 6.0 6.5 for substrates tetramethylbenzidine, guaiacol, caffeic acid, chlorogenic respectively. Inoculation with water or wild-type hrp strains P....
Tendon ruptures are increasingly common, repair can be difficult, and healing is poorly understood. Tissue engineering approaches often require expansion of cell numbers to populate a construct, maintenance phenotype essential for tissue regeneration. Here, we characterize the human Achilles tenocytes assess how this affected by passaging. Tenocytes, isolated from tendon samples 6 patients receiving surgery rupture tendon, were passaged 8 times. Proliferation rates morphology recorded at...
Abstract Purpose Low fruit and vegetable consumption is linked with an increased risk of death from vascular disease cancer. The benefit eating fruits vegetables attributed in part to antioxidants, vitamins phytochemicals. Whether increasing intake impacts on markers remains be established. This study investigates whether daily fruits, juices low (approx. 3 portions), high intakes 8 portions) nutritional clinical biomarkers. Barriers achieving the recommended are also investigated. Method In...
Both wild-type (S21-WT) and hrpD- (S21-533) strains of Pseudomonas syringae pv phaseolicola induced the formation large paramural papillae in lettuce (Lactuca sativa) mesophyll cells adjacent to bacterial colonies. Localized alterations plant cell wall included deposition hydroxyproline-rich glycoproteins, phe-nolics, callose, were associated with proliferation endoplasmic reticulum multivesicular bodies. Tissue collapse during hypersensitive reaction caused by S21-WT was electrolyte leakage...
Dietary strategies for alleviating health complications associated with type 2 diabetes (T2D) are being pursued as alternatives to pharmaceutical interventions. Berries such bilberries (Vaccinium myrtillus L.) that rich in polyphenols may influence carbohydrate digestion and absorption thus postprandial glycaemia. In addition, berries have been reported alter incretins well antioxidant anti-inflammatory properties also affect The present study investigated the acute effect of a standardised...
Lipid and protein oxidation decreases the shelf-life of foods may result in formation end-products potentially detrimental for health. Consumer pressure to decrease use synthetic phenolic antioxidants has encouraged identification alternative compounds or extracts from natural sources. We have assessed whether inclusion dried vegetable powders improves oxidative stability turkey meat patties. Such are not only potentially-rich sources antioxidants, but also impart additional health benefits,...
Abstract The rise of food security up international political, societal and academic agendas has led to increasing interest in novel means improving primary production reducing waste. There are however, also many ‘post-farm gate’ activities that critical security, including processing, packaging, distributing, retailing, cooking consuming. These all affect a range important elements, notably availability, affordability other aspects access, nutrition safety. Addressing the challenge...
Summary The expression of resistance to Bremia lactucae determined by the genes Dm5/8 and Dm7 in lettuce was examined; incompatibility involved hypersensitive reaction (HR) which occurred only within penetrated cells at early late stages fungal development, respectively. Autofluorescence observed under UV blue light excitation undergoing HR associated with accumulation ester‐linked syringaldehyde caffeic acid on plant cell walls. Two phases phenolic deposition were identified. first highly...
The present article describes the second part of a study investigating effect adding vegetables on nutritional, physico-chemical, and oxidative properties wheat bread, specifically focuses bread that does not contain oil as an added ingredient. Wheat flour breads fortified with freeze-dried carrot, tomato, beetroot or broccoli were developed assessed for their nutritional composition, antioxidant potential, stability, storage properties. Using simulated in vitro model, also examined impact...
Summary The role of salicylic acid (SA) in events occurring before cell death during the hypersensitive reaction (HR) was investigated leaves wild‐type tobacco Samsun NN and transgenic lines expressing salicylate hydroxylase (35S‐SH‐L). Challenge 35S‐SH‐L with avirulent strains Pseudomonas syringae gave rise to symptoms resembling those normally associated a compatible response virulent terms visible phenotype, kinetics bacterial multiplication, escape from infection site. Compared responses...
This study investigated the effect of a three week supplementation bilberry extract on glucose metabolism. Overweight volunteers (n = 16; BMI 31) were given capsules either 0.47 g (36% (w/w) anthocyanins) or placebo per day for weeks in double blinded cross over intervention. The ingestion decreased OGTT AUCi (20%; p 0.008) but not insulin compared with placebo. There was no change anti-inflammatory markers (hsCRP, MCP-1, leptin), vascular health (Apo A1, sVCAM-1, sICAM-1), reducing...
The polyacetylene falcarinol, isolated from carrots, has been shown to be protective against chemically induced colon cancer development in rats, but the mechanisms are not fully understood. In this study CaCo-2 cells were exposed falcarinol (0.5−100 μM) and effects on proliferation, DNA damage, apoptosis investigated. Low-dose exposure (0.5−10 decreased expression of indicator caspase-3 concomitantly with basal strand breakage. Cell proliferation was increased (1−10 μM), whereas cellular...
There is increasing emphasis on reformulating processed foods to make them healthier. This study for the first time comprehensively investigated effects of fortifying bread (containing oil as an ingredient) with freeze-dried vegetables its nutritional and physico-chemical attributes. Breads fortified carrot, tomato, beetroot or broccoli were assessed nutrition, antioxidant potential, storage life, shelf stability, textural changes macronutrient oxidation. Furthermore, using in vitro model...
Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins pathways affected by deficiency in human colonocytes. Folate differentially altered activity expression of involved proliferation [e.g., PCNA], DNA repair XRCC5, MSH2], apoptosis BAG family chaperone protein, DIABLO porin], cytoskeletal organization actin, ezrin, elfin], implicated malignant transformation [COMT, Nit2].
Abstract Abstract: Prostate cancer is the second most common in men. The disease etiology poorly understood, but diet and lifestyle are contributory factors. Conjugated linoleic acids (CLAs), naturally occurring fatty ruminant food products, have antitumor properties animal models of antiproliferative effects on cells vitro. cellular mechanisms by which CLAs elicit these unclear, particularly for prostate cells. We previously identified protein kinase C (PKC) isoforms α, δ, ι, μ, ζ LNCaP...