A5008914465- Gout, Hyperuricemia, Uric Acid
- Inflammasome and immune disorders
- Osteoarthritis Treatment and Mechanisms
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Exercise and Physiological Responses
- Case Reports on Hematomas
- Liver Disease Diagnosis and Treatment
- Occupational Health and Performance
- Bone and Joint Diseases
- Advanced Power Generation Technologies
- Inflammatory mediators and NSAID effects
- S100 Proteins and Annexins
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Venous Thromboembolism Diagnosis and Management
- Cancer-related Molecular Pathways
- Advanced Glycation End Products research
- Alcohol Consumption and Health Effects
- Cardiovascular and exercise physiology
- Heat shock proteins research
- Autoimmune and Inflammatory Disorders Research
- Alzheimer's disease research and treatments
- Spondyloarthritis Studies and Treatments
- Geochemistry and Elemental Analysis
Instituto Nacional de Rehabilitación
2015-2024
Secretaria de Salud
2015-2020
Universidad Autónoma Metropolitana
2012-2015
Ferrovial (United Kingdom)
2014
Gout is the most common inflammatory arthropathy of metabolic origin and it characterized by intense inflammation, underlying mechanisms which are unknown. The aim this study was to evaluate oxidative stress in human fibroblast-like synoviocytes (FLS) exposed monosodium urate (MSU) crystals, trigger an process.Human FLS isolated from synovial tissue explants were stimulated with MSU crystals (75 μg/mL) for 24 h. Cellular viability evaluated crystal violet staining, apoptosis assessed using...
Background/Objectives: Aminoglycosides (AG) have been extensively used to treat bacterial diseases for decades. Nevertheless, despite their effectiveness, several secondary adverse effects (auditory, renal, and neurological) evident. Although the mechanisms of these side AG are still unclear, there is a consensus that megalin drug receptor participates in endocytosis. We aimed explore interaction between AGs explain ototoxicity effect by combining silico studies cell functionality assays....
Abstract Mutations in p53 are strongly associated with several highly malignant cancer phenotypes but its role regulating energy metabolism has not been completely elucidated. The effect on glycolysis and oxidative phosphorylation (OxPhos) of mutant R248Q overexpression HeLa cells (HeLa‐M) was analyzed compared overexpressing wild‐type (HeLa‐H) nontransfected containing negligible levels (HeLa‐L). induced early cell detachment during vitro growth; however, detached HeLa‐M showed high...
Osteoarthritis (OA) is a multifactorial degenerative condition of the whole joint with complex pathogenesis whose development and progression significantly mediated by interactions between cartilage articular tissues, particularly, proinflammatory mediators oxidative stress, which results in deterioration subchondral bone destruction. HIF-1 alpha regulates oxygen homeostasis hypoxic tissues such as cartilage; efficiency transcriptional activity HIF1A gene strongly influenced presence...
Even though smoking has been scarcely studied in osteoarthritis (OA) etiology, it is considered a controversial risk factor for the disease. Exposure to tobacco smoke reported promote oxidative stress (OS) as part of damage mechanism. The aim this study was assess whether increases cartilage through generation OS. Peripheral blood (PB) and synovial fluid (SF) samples from patients with OA were analyzed. stratified according habit, Kellgren–Lawrence score, pain, cotinine concentrations PB....
The receptor for advanced glycation end products (RAGE) is a pattern-recognition involved in neurodegenerative and inflammatory disorders. RAGE induces cellular signaling upon binding to variety of ligands. Evidence suggests that up-regulation quinolinate (QUIN)-induced toxicity. We investigated the QUIN-induced toxic events associated with early noxious responses, which might be linked cascades leading cell death. extent damage caused by this rat striatum was characterized image processing...
Background and Objectives: Healthcare workers (HCWs) play important roles in mitigating the COVID-19 pandemic are more likely to become infected with COVID-19. Mexico, among other countries, had a high incidence prevalence of cases deaths from this disease. Material Methods: This retrospective study evaluated clinical characteristics as well geographical distribution cases, deaths, active HCWs non-HCWs using official information Ministry Health Mexico. Results: A total 235,343 were reported...
ABSTRACT Oxidative stress has been recognized as a potential mediator of cell death. Astrocytes play an active role in brain physiology responding to harmful stimuli by activating astrogliosis, which turn associated either with survival or degenerative events. The characterization the mechanistic actions exerted different toxins astrocytes is essential understand function and pathology. As age plays critical processes, aim this study was determine whether administration equimolar...
The ABCG2, SLC22A12, and ALPK1 genes have been strongly associated with dysfunction of urate metabolism in patients gout, but it is unknown how these transporters are expressed acute or chronic gout. Our objectives were to: (a) analyze the gene expression inflammation peripheral blood from gout controls; (b) determine whether metabolic profile can influence transporters, ABCG2 molecules, IL-1β, leukocytes patients; (c) compare them their people without hyperuricemia.A total 36 52 controls...
Among NSAIDs acetyl salicylic acid remains as a valuable tool because of the variety benefic prophylactic and therapeutic effects. Nevertheless, molecular bases for these responses have not been complete understood. We explored effect on heat shock response.Peripheral blood mononuclear cells from rats challenged with presented faster kinetics expression HSP-72 messenger RNA protein in response to vitro shock. This reaches its maximum 2 h after treatment disappeared 5 h. On isolated...
<h3>Background</h3> The presence of subclinical inflammation promotes the progression morphostructural joint damage in RA patients. Currently, clinical remission is based on count swollen and painful joints associated with acute phase reactants. Adding an image technique like grey scale (GS) US power Doppler (PD) increases capability to detect active synovitis. There are no studies association between synovitis as defined by PD more specific markers cytokines patients <h3>Objectives</h3> To...
<h3>Background</h3> Gout is a common type of arthritis that has incresed in the world due to environmental and genetic factors associate it with an altered metabolic state. In Mexico, overweight, obesisty, diabetes syndrome have children adult population, These alterations are associated development gout adults. The characterized by inflammatory chronic process caused deposition monosodium urate crystals (CUMs) joints. recognition these macrophages neutrophils joint activates innate...
The use of nanometric systems to deliver biologically active substances is a successful tool in different fields. In this study, we investigated with antioxidant capacity modulate events associated the redox state human chondrocytes. We used nanoparticles (NPs) prepared chitosan and glutathione (GSH) an vitro model: primary cultures chondrocytes were extracted from hyaline cartilage. cells exposed CdCl₂ presence or absence NPs. widely known oxidizing agent. Fluorescence confocal microscopy...
<h3>Background:</h3> Gout is a multifactorial metabolic disease, characterized by intra articular pain attacks due to inflammatory activation induced monosodium urate (MSU) crystals. These crystals accumulate the patient's frequent hyperuricemia. ABCG2 membrane transporter protein that clears uric acid mainly in intestine and kidney. <i>ABCG2</i> gene has been related hyperuricemia gout polymorphism studies different populations, however its role development progression of disease not clear,...
Background Diagnosing periprosthetic joint infection (PJI) remains a significant challenge for healthcare professionals. Commonly utilized inflammatory markers include erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and white blood cells (WBC). Human β -defensin 1 ( -defensin) is an antimicrobial peptide elevated in infection, yet its diagnostic value PJI has not been explored. The purpose of this study was to evaluate the efficacy synovial as marker compare performance with...
<h3>Background:</h3> Calcium pyrophosphate crystals (CPP) are associated with cartilage lesions and increased severity of osteoarthritis (OA) can induce an inflammatory process. Oxidative stress (OS) is joint inflammation destruction, reactive oxygen species (ROS) mediators damage in OA. <h3>Objectives:</h3> To identify the oxidative characteristics intra-articular CPP deposits their association degree knee <h3>Methods:</h3> Synovial fluid (SF) without were collected analyzed from patients...