A5050718021- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Silk-based biomaterials and applications
- Silkworms and Sericulture Research
- Insect Resistance and Genetics
- Aquaculture Nutrition and Growth
- Insect and Arachnid Ecology and Behavior
- Physiological and biochemical adaptations
- Invertebrate Immune Response Mechanisms
- MicroRNA in disease regulation
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive biology and impacts on aquatic species
- GABA and Rice Research
- Plant responses to water stress
- Wound Healing and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Power Systems Fault Detection
- Catalytic Processes in Materials Science
- 14-3-3 protein interactions
- CRISPR and Genetic Engineering
- Electrical Fault Detection and Protection
- Insect-Plant Interactions and Control
- Microbial Natural Products and Biosynthesis
- Genetics, Aging, and Longevity in Model Organisms
Southwest University
2007-2025
State Key Laboratory of Silkworm Genomic Biology
2012-2020
China West Normal University
2020
Abstract Background MicroRNA (miRNA) and other small regulatory RNAs contribute to the modulation of a large number cellular processes. We sequenced three RNA libraries prepared from whole body, anterior-middle posterior silk glands Bombyx mori , with view expanding repertoire silkworm miRNAs exploring transcriptional differences in between segments gland. Results With aid large-scale Solexa sequencing technology, we validated 257 unique miRNA genes, including 202 novel 55 previously...
Significance Females of several mosquito species have evolved obligatory blood-feeding to supply amino acids and other nutrients necessary for egg development, providing a mechanism the spread many devastating diseases in humans. The mosquito-specific microRNA (miRNA), miR-1174, is significantly upregulated post blood meal specific female midgut, suggesting role meal-associated events. We found that miR-1174 targets serine hydroxymethyltransferase required key functions Aedes aegypti...
Abstract Background MicroRNAs (miRNAs) repress target genes at the post-transcriptional level, and function in development cell-lineage pathways of host species. Tissue-specific expression miRNAs is highly relevant to their physiological roles corresponding tissues. However, date, few have been spatially identified silkworm. Results We establish for first time spatial patterns nearly 100 multiple normal tissues (organs) Bombyx mori females males using microarray Northern-blotting analyses....
Abstract Background lin-4 and let-7 , the two founding members of heterochronic microRNA genes, are firstly confirmed in Caenorhabditis elegans to control proper timing developmental programs a pathway. has been thought trigger onset adulthood across animal phyla. Ecdysone Broad-Complex required for temporal expression Drosophila melanogaster . For better understanding conservation functions we seek explore how it is expressed silkworm ( Bombyx mori ). Results One member family identified...
Vitellogenin receptors (VgRs) play crucial roles in oogenesis by mediating endocytosis of vitellogenin and other nutrients ovipara. We conducted small RNA sequencing screening with a luciferase reporter system, found that bmo-miR-2739 novel miRNA (novel-miR-167) coordinately regulate the expression VgR Bombyx mori (BmVgR). Further analyses suggested these two miRNAs direct target repression binding directly to BmVgR 3' untranslated region. Forced either using piggyBac system blocked (Vg)...
20-Hydroxyecdysone (20-HE) plays essential roles in coordinating developmental transitions of insects through responsive protein-coding genes and microRNAs (miRNAs). The involvement single miRNAs the ecdysone-signalling pathways has been extensively explored, but interplay between ecdysone majority still remains largely unknown. Here, by small RNA sequencing, we systematically investigated genome-wide responses to 20-HE embryogenic cell lines Bombyx mori Drosophila melanogaster. Over 60 70...
Despite more than 40 years of intense study, essential features the silkmoth chorion (eggshell) are still not fully understood. To determine precise structure locus, we performed extensive EST analysis, constructed a bacterial artificial chromosome (BAC) contig, and obtained continuous genomic sequence 871,711 base pairs. We annotated 127 genes in two segments interrupted by 164 kb region with 5 non-chorion genes, orthologs which were on bearing scaffolds 4 ditrysian families. Detailed...
Mannosylerythritol lipids (MELs) are a class of glycolipids that have been receiving increasing attention in recent years due to their diverse biological activities. MELs produced by certain fungi and display range bioactivities, making them attractive candidates for various applications medicine, agriculture, biotechnology. Despite remarkable qualities, industrial-scale production remains challenge fungal strains. Excellent strains fermentation processes essential the efficient MELs, so...
The silk gland is characterized by high protein synthesis. However, the molecular mechanisms controlling growth and synthesis remain undetermined. Here we demonstrated that CRISPR/Cas9-based knockdown of let-7 or whole cluster promoted endoreduplication enlargement gland, accompanied changing yield, whereas transgenic overexpression led to atrophy degeneration gland. Mechanistically, controls cell in through coordinating nutrient metabolism processes energy signalling pathways. Transgenic...
Pathogenic viruses can be transmitted by female Aedes aegypti (Ae. aegypti) mosquitoes during blood-meal acquisition from vertebrates. Silencing of mosquito- and midgut-specific microRNA (miRNA) 1174 (miR-1174) impairs blood intake increases mortality. Determining the identity proteins metabolites that respond to miR-1174 depletion will increase our understanding molecular mechanisms this miRNA in controlling blood-feeding nutrient metabolism mosquitoes. Antisense oligonucleotides...
Abstract Background Female Aedes aegypti mosquitoes are vectors of arboviruses that cause diverse diseases public health significance. Blood protein digestion by midgut proteases provides anautogenous with the nutrients essential for oocyte maturation and egg production. Midgut-specific miR-1174 affects functions through its target gene serine hydroxymethyltransferase ( SHMT ). However, less is known about -regulated processes in blood mosquitoes. Methods RNAi was realized injection...
MicroRNAs (miRNAs) are a class of non-coding RNAs with important post-transcriptional regulatory functions. To reveal the function miRNAs in vivo, critical step is to change their expression levels tissues or organs. In this work, we explored application several genetic techniques altering silk gland-specific miR-274 silkworm (Bombyx mori).Injection synthesized microRNA mimics and antagomirs exerted no effect on gland, sponge specifically absorbed down-regulated its expression, transgenic...
The silk gland of the silkworm Bombyx mori serves as a valuable model for investigating morphological structure and physiological functions organs. Previous studies have demonstrated notable regulatory role let-7 microRNA in gland, but its specific molecular mechanism remains to be elucidated across different segments this organ. In study, we further investigated functional middle (MSG). MSG knockout strain was analyzed using combined proteomic metabolomic technique, revealing enrichment...
The heterochronic genes regulate cell proliferation and switch development stage transitions. Heterochronic might also play important roles in regulating the of silkworm, but very few their expression profiles, functions relationship with microRNAs are available so far. Firstly, this work, primers for cloning Bmlin-41 were designed based on homologous sequence known Drosophila melanogaster lin-41, which was used as query to blast against SilkDB. obtained full CDS (2 166 bp) encodes 721 amino...