A5053205946- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Stress Responses and Cortisol
- Ion channel regulation and function
- Sleep and Wakefulness Research
- Spinal Cord Injury Research
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Amyotrophic Lateral Sclerosis Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Child and Adolescent Psychosocial and Emotional Development
- Pain Mechanisms and Treatments
- Neurogenetic and Muscular Disorders Research
- Neonatal and fetal brain pathology
- Menstrual Health and Disorders
- Memory and Neural Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Adipose Tissue and Metabolism
- Pharmacological Effects and Toxicity Studies
- Neural dynamics and brain function
Université de Bordeaux
2015-2025
Institut de Neurosciences Cognitives et Intégratives d’Aquitaine
2013-2025
Centre National de la Recherche Scientifique
2015-2024
École Pratique des Hautes Études
2021-2024
Centre Hospitalier Universitaire de Bordeaux
2005
Université de Montréal
2001-2003
Inserm
2003
Neurocentre Magendie
2002
Merck Canada Inc. (Canada)
2001
MSD (UK) Limited (United Kingdom)
2001
γ-Aminobutyric acid (GABA) activates two qualitatively different inhibitory mechanisms through ionotropic GABA<sub>A</sub> multisubunit chloride channel receptors and metabotropic GABA<sub>B</sub> G protein-coupled receptors. Evidence suggests that pharmacologically distinct receptor subtypes mediate presynaptic inhibition of neurotransmitter release by reducing Ca<sup>2+</sup>conductance, postsynaptic neuronal excitability activating inwardly rectifying K<sup>+</sup> (Kir) conductance....
The maintenance of chronic pain states requires the regulation gene expression, which relies on an influx calcium. Calcium through neuronal L-type voltage-gated calcium channels (LTCs) plays a pivotal role in excitation-transcription coupling, but involvement LTCs remains unclear. We used peptide nucleic acid (transportan 10-PNA conjugates)-based antisense strategy to investigate LTC subtypes Ca(V)1.2 and Ca(V)1.3 long-term sensitization rat model neuropathy (spinal nerve ligation). Our...
Substance-induced social behavior deficits dramatically worsen the clinical outcome of substance use disorders; yet, underlying mechanisms remain poorly understood. Herein, we investigated role for corticotropin-releasing factor receptor 1 (CRF ) in acute sociability induced by morphine and related activity oxytocin (OXY)-and arginine-vasopressin (AVP)-expressing neurons paraventricular nucleus hypothalamus (PVN). For this purpose, used both CRF receptor-preferring antagonist compound...
Substance-induced social behavior deficits dramatically worsen the clinical outcome of substance use disorders; yet, underlying mechanisms remain poorly understood. Herein, we investigated role for corticotropin-releasing factor receptor 1 (CRF ) in acute sociability induced by morphine and related activity oxytocin (OXY)- arginine-vasopressin (AVP)-expressing neurons paraventricular nucleus hypothalamus (PVN). For this purpose, used both CRF receptor-preferring antagonist compound...
Repetitive stimulation of Schaffer collaterals induces activity-dependent changes in the strength polysynaptic inhibitory postsynaptic potentials (IPSPs) hippocampal CA1 pyramidal neurons that are dependent on parameters. In present study, we investigated effects two patterns, theta-burst (TBS) and 100 Hz tetani, pharmacologically isolated monosynaptic GABAergic responses adult cells. Tetanization with trains transiently depressed both early late IPSPs, whereas TBS induced long-term...
Bertrand, Sandrine and Jean-René Cazalets. Postinhibitory rebound during locomotor-like activity in neonatal rat motoneurons vitro. J. Neurophysiol. 79: 342–351, 1998. The aim of this study was to establish how a membrane property contributes the neuronal discharge ongoing behavior. We therefore studied role postinhibitory (PIR) bursting lumbar intracellularly recorded newborn vitro brain stem/spinal cord preparation. PIR is transient depolarization that occurs after hyperpolarization. first...
Large cholinergic synaptic terminals known as C-boutons densely innervate the soma and proximal dendrites of motoneurons that are prone to neurodegeneration in amyotrophic lateral sclerosis (ALS). Studies using Cu/Zn-superoxide dismutase (SOD1) mouse model ALS have generated conflicting data regarding C-bouton alterations exhibited during pathogenesis. In present work, a longitudinal study combining immunohistochemistry, biochemical approaches extra- intra-cellular electrophysiological...
Abstract We have studied the rhythm‐generating capabilities of lumbar, sacral and coccygeal (Co) areas using an isolated spinal cord preparation newborn rat. The bath‐application a mixture N‐methyl‐ d ‐ l ‐aspartate (NMA) serotonin (5‐HT) on whole induced coordinated rhythmic activity that could be recorded from lumbar to ventral roots. phase relationships mean burst duration between in rostral segments was analysed. direct activation network, by sections or selective pharmacological...
Abstract Gabapentin is a clinically effective anticonvulsant with an unclear mechanism of action. It was described as GABA B(1a,2) receptor subtype‐selective agonist, activating postsynaptic K + currents and inhibiting Ca 2+ channels in CA1 pyramidal cells, but without presynaptic actions. These activities appeared controversial we therefore sought to further clarify gabapentin actions rat hippocampal slices by characterizing targeted using whole‐cell recording multiphoton imaging. 1) We...
Acetylcholine and the activation of muscarinic receptors influence activity neural networks generating locomotor behavior in mammalian spinal cord. Using electrical stimulations ventral commissure, we show that commissural (CM) depolarizations could be induced lumbar motoneurons. We provide a detailed electrophysiological characterization membrane conductance involved these responses. Activation CM terminals, originating from lamina X neurons partition cells, pathway-specific short-term...
Abstract Amyotrophic lateral sclerosis (ALS) is a fatal motoneuron (MN) disease characterized by protein misfolding and aggregation leading to cellular degeneration. So far neither biomarker, nor effective treatment has been found. ATP signaling P2X4 receptors (P2X4) are upregulated in various neurodegenerative diseases. Here we show that several ALS-related misfolded proteins including mutants of SOD1 or TDP-43 lead significant increase surface receptor density function vitro. In addition,...
Abstract Various studies on isolated neonatal rat spinal cord have pointed to the predominant role played by rostral lumbar area in generation of locomotor activity. In present study, various regions genesis was further examined using compartmentalization and transections cord. We report that synaptic drive received caudal motoneurons following N ‐methyl‐ d ‐ l ‐aspartate (NMA)/5‐HT superfusion entire is different from triggered same compounds specifically applied segments. These differences...
Effective quadrupedal locomotor behaviors require the coordination of many muscles in limbs, back, neck, and tail. Because spinal motoneuronal somatotopic organization, motor implies interactions among distant networks. Here, we investigated some between lumbar networks that control limb movements thoracic axial involved trunk movement. For this purpose, used an vitro isolated newborn rat cord (from T2 to sacrococcygeal) preparation. Using extracellular ventral root recordings, showed that,...
1 Unitary inhibitory postsynaptic currents (uIPSCs) were characterised between 23 synaptically coupled interneurones at the border of stratum radiatum and lacunosum-moleculare (LM) CA1 pyramidal cells (PYR) using dual whole-cell recordings morphological identification in rat hippocampal slices. 2 LM presented a morphology typical stellate cells, with fusiform soma as well dendritic axonal arborisations lacunosum-moleculare. 3 Single spikes triggered uIPSCs that blocked by GABAA antagonist...
In the present study, we investigated modulation of short-term depression (STD) at synapses between sensory afferents and rat motoneurons by serotonin, dopamine noradrenaline. STD was elicited with trains 15 stimuli 1, 5 10 Hz using whole-cell voltage-clamp recordings from identified in neonatal spinal cord vitro. differentially modulated amines. Dopamine effective all stimulation frequencies, whereas serotonin affected only during stimulus noradrenaline 1 trains. homogenized degree observed...