A5071158988- Parasite Biology and Host Interactions
- Antimicrobial Peptides and Activities
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Invertebrate Immune Response Mechanisms
- Antimicrobial agents and applications
- Biochemical Analysis and Sensing Techniques
- Biopolymer Synthesis and Applications
- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- Insect-Plant Interactions and Control
- Marine and fisheries research
- Forest Insect Ecology and Management
- Identification and Quantification in Food
- Venomous Animal Envenomation and Studies
- interferon and immune responses
- Toxic Organic Pollutants Impact
- Enzyme Structure and Function
- Pharmacogenetics and Drug Metabolism
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- Insect and Arachnid Ecology and Behavior
- Biochemical and Structural Characterization
Université Laval
2014-2024
University of Birmingham
2023
Fisheries and Oceans Canada
2016
Bioénergétique et Ingénierie des Protéines
1993
Centre National de la Recherche Scientifique
1993
Investigating how environmental features shape the genetic structure of populations is crucial for understanding they are potentially adapted to their habitats, as well sound management. In this study, we assessed relative importance spatial distribution, ocean currents and sea surface temperature (SST) on patterns putatively neutral adaptive variation among American lobster from 19 locations using population differentiation (PD) approaches combined with association (EA) analyses. First, PD...
The International Pseudomonas aeruginosa Consortium is sequencing over 1000 genomes and building an analysis pipeline for the study of genome evolution, antibiotic resistance virulence genes. Metadata, including genomic phenotypic data each isolate collection, are available through Database (http://ipcd.ibis.ulaval.ca/). Here, we present our strategy results that emerged from first 389 genomes. With as yet unmatched resolution, confirm P. strains can be divided into three major groups...
Small heat shock proteins (sHSPs) are oligomeric stress characterized by an α-crystallin domain (ACD) surrounded a N-terminal arm and C-terminal extension. Publications on sHSPs have reported that they exist in prokaryotes eukaryotes but, to our knowledge, not viruses. Here we show present some cyanophages infect the marine unicellular cyanobacteria, Synechococcus Prochlorococcus. These phage contain conserved ACD flanked relatively short extension with or without anchoring module (CAM)...
Summary Periodic outbreaks of spruce budworm ( SBW ) affect large areas ecologically and economically important conifer forests in North America, causing tree mortality reduced forest productivity. Host resistance against has been linked to growth phenology the chemical composition foliage, but underlying molecular mechanisms population variation are largely unknown. Using a genomics approach, we discovered β‐glucosidase gene, Pgβglu‐1 , whose expression levels function underpin natural...
Abstract Using massively parallel sequencing data from two species with different life history traits, American lobster ( Homarus americanus ) and Arctic Char Salvelinus alpinus ), we highlight how an unbalanced sex ratio in the samples a few sex‐linked markers may lead to false interpretations of population structure thus potentially erroneous management recommendations. Here, multivariate analyses revealed genetic clusters separating by instead expected spatial variation: inshore offshore...
Abstract The gypsy moth, Lymantria dispar L., is one of the most destructive forest pests in world. While subspecies established North America European moth ( L. ), whose females are flightless, two Asian subspecies, asiatica and japonica , have flight-capable females, enhancing their invasiveness warranting precautionary measures to prevent permanent establishment America. Various molecular tools been developed help distinguish from several which based on mitochondrial barcode region. In an...
Abstract Major histocompatibility ( MHC ) immune system genes may evolve in response to pathogens the environment. Because they also affect mate choice, are candidates for having great importance ecological speciation. Here, we use next‐generation sequencing test general hypothesis of parallelism patterns MHCII β diversity and bacterial infections among five dwarf normal whitefish sympatric pairs. A second objective was assess functional relationships between specific alleles natural...
Abstract Using massively parallel sequencing data from two species with different life history traits -- American lobster ( Homarus americanus ) and Arctic Char Salvelinus alpinus we highlighted how an unbalanced sex ratio in the samples combined a few sex-linked markers may lead to false interpretations of population structure thus potentially erroneous management recommendations. Multivariate analyses revealed genetic clusters that separated males females instead showing expected pattern...
Manipulative parasites are thought to liberate molecules in their external environment, acting as manipulation factors with biological functions implicated host's physiological and behavioural alterations. These part of a complex mixture called the secretome. While secretomes various have been described, there is very little data for putative manipulative parasite. It necessary study molecular interaction between parasite its host better understand how such alterations evolve.Here, we used...
ABSTRACT Recent studies demonstrated that 2,3-dihydroxybiphenyl 1,2-dioxygenase from Burkholderia sp. strain LB400 (DHBD ; EC 1.13.11.39) cleaves chlorinated 2,3-dihydroxybiphenyls (DHBs) less specifically than unchlorinated DHB and is competitively inhibited by 2′,6′-dichloro-2,3-dihydroxybiphenyl (2′,6′-diCl DHB). To determine whether these are general characteristics of DHBDs, we characterized DHBD P6 -I -III, two evolutionarily divergent isozymes Rhodococcus globerulus P6, another good...
Mouse prostate membrane-associated proteins of the annexin family showed changes in SUMOylation during androgen treatment. Among these calcium-binding A1 protein (ANXA1) was chosen for further characterization given its role secretion and cancer. ANXA1 confirmed by overexpressing SUMO-1 LNCaP cells. Site-directed mutagenesis indicated that K257 located a consensus motif C-terminal DA3 repeat domain is SUMOylated. Mutation N-terminal Y21 decreased markedly signal while EGF stimulation...
Abstract Spike (S) protein is a key in coronaviruses life cycle. SARS-CoV-2 Omicron BA.1 variant of concern (VoC) presents an exceptionally high number 30 substitutions, 6 deletions and 3 insertions the S protein. Recent works revealed major changes biological properties compared to earlier variants (VoCs). Here, these could be explained, at least part, by mutations N764K and/or N856K S2 subunit. These were not previously detected other VoCs. generate two potential cleavage sites for...
The enzyme kinetics of the amide ligase MurE, a cell wall biosynthesis enzyme, from Pseudomonas aeruginosa were determined using synthesized nucleotide substrate UDP-MurNAc-Ala-Glu (uridine 5'-diphosphoryl N-acetylmuramoyl-L-alanyl-D-glutamate). When coupled to competitive bio-panning technique M13 phage display library encoding approximately 2.7 x 10(9) random peptide permutations and specific substrates meso-A2pm (meso-diaminopimelic acid) ATP, inhibitor MurE was identified. MurEp1...
ABSTRACT The ability of the dehydrogenase and ring cleavage dioxygenase naphthalene degradation pathway to transform 3,4-dihydroxylated biphenyl metabolites was investigated. 1,2-Dihydro-1,2-dihydroxynaphthalene expressed as a histidine-tagged protein. purified enzyme transformed 2,3-dihydro-2,3-dihydroxybiphenyl, 3,4-dihydro-3,4-dihydroxybiphenyl, 3,4-dihydro-3,4-dihydroxy-2,2′,5,5′-tetrachlorobiphenyl 2,3-dihydroxybiphenyl, 3,4-dihydroxybiphenyl (3,4-DHB),...
ABSTRACT HIV-1 acquires an impressive number of foreign components during its formation. Despite all previous efforts spent studying the nature and functionality virus-anchored host molecules, exact mechanism(s) through which such constituents are acquired by is still unknown. However, in case ICAM-1, one most extensively studied transmembrane proteins found associated with mature virions, Pr55 Gag precursor polyprotein appears to be a potential interaction partner. We investigated...
Glycoside hydrolase family 32 (GH32) enzymes cleave the glycosidic bond between two monosaccharides or a carbohydrate and an aglycone moiety. GH32 have been studied in prokaryotes eukaryotes but not viruses. This is first analysis of Bacillus subtilis phage SP10, ϕNIT1 SPG24. Phylogenetic analysis, molecular docking secretability predictions suggest that function as levan (fructose homopolysaccharide) fructotransferase. We showed viruses also contain our analyses silico strongly these
Identifying the molecular changes that lead to ecological specialization during speciation is one of major goals evolution. One question remains be thoroughly investigated whether derives strictly from adaptive and their associated trade-offs, or conditionally neutral mutations accumulate under relaxed selection. We used whole-genome sequencing, genome annotation computational analyses identify genes have rapidly diverged between two incipient species Saccharomyces paradoxus occupy different...
Abstract Insects have developed various adaptations to survive harsh winter conditions. Among freeze-intolerant species, some produce “antifreeze proteins” (AFPs) that bind nascent ice crystals and inhibit further growth. Such is the case of spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae), a destructive North American conifer pest can withstand temperatures below −30°C. Despite potential importance AFPs in adaptive diversification Choristoneura, genomic tools explore...
Cecropins form a family of amphipathic α-helical cationic peptides with broad-spectrum antibacterial properties and potent anticancer activity. The emergence bacteria cancer cells showing resistance to antimicrobial (CAMPs) has fostered search for new, more selective effective alternatives CAMPs. With this goal in mind, we looked cecropin homologs the genome transcriptome spruce budworm, Choristoneura fumiferana. Not only did find paralogs conventional cecropins (Cfcec+ ), our screening also...
The small Hsp DmHsp27 from Drosophila melanogaster is one of the few heat shock proteins (sHsps) found within nucleus. We report that its dimerization independent disulfide bond formation and seems to rely on salt bridges. Unlike metazoan sHsps, forms two populations oligomers not in equilibrium. Mutations at highly conserved arginine residues mammalian sHsps have been reported be associated with protein conformational defects intracellular aggregation. Independent mutation three arginines...