A5042518019- Metabolomics and Mass Spectrometry Studies
- Advanced MRI Techniques and Applications
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Urinary Tract Infections Management
- Metabolism and Genetic Disorders
- Cancer, Lipids, and Metabolism
- Cardiac Imaging and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Bacterial Identification and Susceptibility Testing
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gallbladder and Bile Duct Disorders
- Adipose Tissue and Metabolism
- Gut microbiota and health
- Diagnosis and treatment of tuberculosis
- Traditional Chinese Medicine Studies
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Advanced Proteomics Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Organ Transplantation Techniques and Outcomes
- Pediatric Urology and Nephrology Studies
- S100 Proteins and Annexins
- Biliary and Gastrointestinal Fistulas
Centre of Biomedical Research
2016-2025
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2015-2024
Post Graduate Institute of Medical Education and Research
2022-2024
Roswell Park Comprehensive Cancer Center
2022-2023
Johns Hopkins University
2009-2022
Johns Hopkins Medicine
2009-2022
Baystate Health
2022
University of Massachusetts Chan Medical School
2022
All India Institute of Medical Sciences Bhopal
2018-2021
All India Institute of Medical Sciences Raipur
2018-2021
ATP is required for normal cardiac contractile function, and it has long been hypothesized that reduced energy delivery contributes to the dysfunction of heart failure (HF). Despite experimental clinical HF data showing metabolism through creatine kinase (CK), major myocardial reserve temporal buffer, a causal relationship between ATP-CK in never demonstrated. Here, we generated mice conditionally overexpressing myofibrillar isoform CK (CK-M) test hypothesis augmenting impaired CK-related...
Abstract Despite the clear association between myocardial injury, heart failure and depressed energetics, little is known about upstream signals responsible for remodeling metabolism after pathological stress. Here, we report increased mitochondrial calmodulin kinase II (CaMKII) activation left ventricular dilation in mice one week infarction (MI) surgery. By contrast, with genetic CaMKII inhibition are protected from dysfunction MI. Mice overexpression (mtCaMKII) have severe dilated...
Despite continuing research for precise probing and grading of prostate cancer (PC) biomarkers, the indexes lack sensitivity specificity. To search PC we used proton nuclear magnetic resonance (1H NMR)-derived serum metabolomics. The study comprises 102 samples obtained from low-grade (LG, n = 40) high-grade (HG, 30) cases healthy controls (HC, 32). 1H NMR-derived data were examined using principal component analysis orthogonal partial least-squares discriminant analysis. strength model was...
Abnormalities in cardiac energy metabolism occur heart failure (HF) and contribute to contractile dysfunction, but their role, if any, HF-related pathologic remodeling is much less established. CK (creatine kinase), the primary muscle reserve reaction which rapidly provides ATP at myofibrils regenerates mitochondrial ADP, down-regulated experimental human HF. We tested hypotheses that HF related impaired rescuing attenuates maladaptive hypertrophy
To address the shortcomings of urine cytology and cystoscopy for probing grading urinary bladder cancer (BC), we applied 1H nuclear magnetic resonance (NMR) spectroscopy as a surrogate method identification BC. This study includes 99 serum samples comprising low-grade (LG; n = 36) high-grade (HG; 31) BC well healthy controls (HC; 32). NMR-derived data were analyzed using orthogonal partial least-squares discriminant analysis (OPLS-DA). OPLS-DA-derived model validity was confirmed an internal...
To address the shortcomings of digital rectal examinations (DRE), serum prostate-specific antigen (PSA), and trans-rectal ultrasound (TRUS) for precise determination prostate cancer (PC) differentiation from benign prostatic hyperplasia (BPH), we applied (1) H-nuclear magnetic resonance (NMR) spectroscopy as a surrogate tactic probing prediction PC BPH.The study comprises 210 filtered sera suspected PC, BPH, healthy subjects' cohort (HC). The approach delineates to identify quantify 52...
Despite the easy and reliable methods of blood pressure measurement, screening essential hypertension (EH) is usually ignored due to delayed onset symptoms. A probe into biochemical changes in would serve as a welcome asset provide insight mechanistic aspects EH. Filtered serum samples from 64 EH patients 59 healthy controls (HC) were analysed using 800 MHz nuclear magnetic resonance (NMR) spectroscopy. Application principal component analysis (PCA) orthogonal partial least-squares...
In spite of ongoing efforts to probe the metabolic signatures stable (SA) from unstable (US) angina, it is concerning that date there are no clinically validated circulatory biochemical against intrinsic anatomical changes screened by invasive coronary angiography. Hence aim this study generate precise fingerprints using filtered serum-based metabolomics and high-throughput NMR spectroscopy accurately distinguish patients suffering with SA or US angina. The includes 118 serum samples (n =...
The hypothesis that the failing heart may be energy-starved is supported in part by observations of reduced rates adenosine 5'-triphosphate (ATP) synthesis through creatine kinase (CK) reaction, primary myocardial energy reservoir, patients with failure (HF). Although murine models have been used to probe HF pathophysiology, it has not possible noninvasively measure rate ATP CK vivo mouse heart. purpose this work was exploit noninvasive spatially localized magnetic resonance spectroscopy...
Doxorubicin (DOX) is a commonly used life-saving antineoplastic agent that also causes dose-dependent cardiotoxicity. Because ATP absolutely required to sustain normal cardiac contractile function and because impaired synthesis through creatine kinase (CK), the primary myocardial energy reserve reaction, may contribute dysfunction in heart failure, we hypothesized CK metabolism contributes DOX-induced We therefore overexpressed myofibrillar isoform of (CK-M) determined energetic, survival...
Adult (3 month) mice with cardiac-specific overexpression of adenylyl cyclase (AC) type VIII (TG AC8 ) adapt to an increased cAMP-induced cardiac workload (~30% increases in heart rate, ejection fraction and output) for up a year without signs failure or excessive mortality. Here, we show classical hypertrophy markers were absent TG , that total left ventricular (LV) mass was not increased: reduced LV cavity volume encased by thicker walls harboring number small myocytes, network...
To address the shortcomings of urine culture for rapid identification urinary tract infection (UTI), we applied 1H-nuclear magnetic resonance (NMR) spectroscopy as a surrogate method fast screening microorganisms. Study includes 682 samples from suspected UTI patients, 50 healthy volunteers, and commercially available standard strains gram negative bacilli (GNB) (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Enterobacter, Acinetobacter, Proteus mirabilis, Citrobacter...
(31)P magnetic resonance spectroscopy (MRS) offers a unique means to noninvasively quantify the major cardiac high-energy phosphates, creatine phosphate (PCr) and adenosine 5'-triphosphate (ATP), that are critical for normal myocardial contractile function viability. Spatially localized MRS has been used in vivo PCr-to-ATP ratio (PCr/ATP) of murine hearts, including those with pressure-overload hypertrophy induced by thoracic aortic constriction (TAC). To date, there no approach measuring...
Reduced myofibrillar ATP availability during prolonged myocardial ischemia may limit post-ischemic mechanical function. Because creatine kinase (CK) is the prime energy reserve reaction of heart and because it has been difficult to augment synthesis after ischemia, we used mice that overexpress isoform (CKM) in cardiac-specific, conditional fashion test hypothesis CKM overexpression increases delivery ischemic-reperfused hearts improves functional recovery. Isolated, retrograde-perfused from...
This article describes proton MR spectroscopic analysis of cerebrospinal fluid 167 children suffering from meningitis and 24 control cases. Quantification 12 well-separated commonly observed metabolites viz., beta-hydroxybutyrate, lactate, alanine, acetate, acetone, acetoacetate, pyruvate, glutamine, citrate, creatine/creatinine, glucose (total) urea was carried out using Bruker's NMRQUANT software with respect to a known concentration sodium-3-(trimethylsilyl)-2,2,3,3-d4-propionate (TSP),...
OBJECTIVE To address the shortcomings of urine culture for diagnosis urinary tract infection (UTI), we used 1 H‐nuclear magnetic resonance (NMR) spectroscopy identifying and quantifying Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia Proteus mirabilis . PATIENTS, SUBJECTS AND METHODS Urine samples from patients with suspected UTI (617), healthy volunteers (50) commercially available standard strains E. K. pneumonia, P. Enterobacter, Acinobacter, Pr. mirabilis, Citrobacter...