A5067824015- Bladder and Urothelial Cancer Treatments
- Urological Disorders and Treatments
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac, Anesthesia and Surgical Outcomes
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Prenatal Substance Exposure Effects
- RNA Interference and Gene Delivery
- Nausea and vomiting management
- Birth, Development, and Health
- Stress Responses and Cortisol
- Anesthesia and Pain Management
- Plant Physiology and Cultivation Studies
- Ureteral procedures and complications
- Surgical Simulation and Training
- Privacy, Security, and Data Protection
- Nuts composition and effects
- Optical Imaging and Spectroscopy Techniques
- Health Systems, Economic Evaluations, Quality of Life
- Urinary and Genital Oncology Studies
- Anesthesia and Sedative Agents
- Prostate Cancer Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Fatty Acid Research and Health
Roswell Park Comprehensive Cancer Center
2020-2025
National Research Council Canada
2011-2025
Icahn School of Medicine at Mount Sinai
2019-2024
Moffitt Cancer Center
2024
Mount Sinai Health System
2023
Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
2016-2023
University of Florida
2023
Washington University in St. Louis
2023
Rashid Latif Medical College
2020-2022
Mayo Hospital
2018
A mouse model of glioblastoma multiforme was used to determine the accumulation a targeted contrast agent in tumor vessels.The agent, consisting superparamagnetic iron oxide coated with dextran, functionalized an anti-insulin-like-growth-factor binding protein 7 (anti-IGFBP7) single domain antibody.The near infrared marker, Cy5.5, also attached for vivo fluorescence study.A 9.4T magnetic resonance imaging (MRI) system studies on days 10 and 11 following inoculation.T 2 relaxation time...
The posttranslational modification of therapeutic proteins with terminal sialic acids is one means improving their circulating half-life, thereby efficiency. We have developed a two-step in vitro enzymatic glycoproteins, which has previously only been achieved by chemical [Gregoriadis G, Jain S, Papaioannou I, Laing P (2005) Int J Pharm 300:125–130). This procedure uses the Campylobacter jejuni Cst-II α2,8-sialyltransferase to provide primer on N-linked glycans, followed polysialylation...
Soricidin is a 54-amino acid peptide found in the paralytic venom of northern short-tailed shrew (Blarina brevicauda) and has been to inhibit transient receptor potential vallinoid type 6 (TRPV6) calcium channels. We report that two shorter peptides, SOR-C13 SOR-C27, derived from C-terminus soricidin, are high-affinity antagonists human TRPV6 channels up-regulated number cancers. Herein, we molecular imaging methods demonstrate vivo diagnostic SOR-C27 target tumor sites mice bearing ovarian...
The blood-brain barrier (BBB) prevents the majority of drugs from crossing into brain and reaching neurons. To overcome this challenge, safe non-invasive technologies targeting receptor-mediated pathways have been developed. In study, three single-domain antibodies (sdAbs; IGF1R3, IGF1R4, IGF1R5) extracellular domain human insulin-like growth factor-1 receptor (IGF1R), generated by llama immunization, showed enhanced transmigration across rat BBB model (SV-ARBEC) in vitro. rate uptake these...
The overexpression of epidermal growth factor receptor (EGFR) and its mutated variant EGFRvIII occurs in 50% glioblastoma multiforme. We developed antibody fragments against EGFR/EGFRvIII for molecular imaging and/or therapeutic targeting applications.An anti-EGFR/EGFRvIII llama single-domain (EG(2)) two higher valency format constructs, bivalent EG(2)-hFc pentavalent V2C-EG(2) sdAbs, were analysed vitro their binding affinities using surface plasmon resonance cell studies, vivo...
mRNA-based therapies can overcome several challenges faced by traditional in treating a variety of diseases selectively modulating genes and proteins without genomic integration. However, due to mRNA's poor stability inherent limitations, nanoparticle (NP) platforms have been developed deliver functional mRNA into cells. In cancer treatment, technology has multiple applications, such as restoration tumor suppressors activating antitumor immunity. Most these applications evaluated using...
Insulin-like growth factor-binding protein 7 (IGFBP7) is an abundant, selective and accessible biomarker of glioblastoma multiforme (GBM) tumour vessels. In this study, anti-IGFBP7 single-domain antibody (sdAb) was developed to target GBM vessels for molecular imaging applications. Human modelled in mice by intracranial implantation U87MG.EGFRvIII cells. An sdAb, isolated from immune llama library panning, assessed vitro its binding affinity using surface plasmon resonance ex vivo...
Abstract Sleep deprivation is a form of stress that provokes both inflammatory responses and neuropsychiatric disorders. Because persistent inflammation implicated as physiological process in anxiety disorders, we investigated the contributions NLRP3 inflammasome signaling to anxiolytic properties flavanol diets model chronic sleep deprivation. The results show flavanol-rich dietary preparation (FDP) exhibits by attenuating markers neuroimmune activation, which included IL-1β upregulation,...
The maintenance of therapeutic glycoproteins within the circulatory system is associated, in large part, with integrity sialic acids as terminal sugars on glycans. Glycoprotein desialylation, either by spontaneous cleavage or through host sialidases, leads to protein clearance, mainly liver. Thus, installation minimally modified that are hydrolysis-resistant yet biologically equivalent should lead increased half-lives and improved pharmacokinetic profiles. Here we describe chemoenzymatic...
Insulin-like growth factor-binding protein 4 (IGFBP-4/IBP-4) has potent IGF-independent anti-angiogenic and antitumorigenic effects. In this study, we demonstrated that these activities are located in the IGFBP-4 C-terminal fragment (CIBP-4), a region containing thyroglobulin type 1 (Tg1) domain. Proteins bearing Tg1 domains have been shown to inhibit cathepsins, lysosomal enzymes involved basement membrane degradation implicated tumor invasion angiogenesis. our studies, CIBP-4 was...
Archaeosomes are liposomes comprised of ether lipids derived from various archaea. Unlike conventional ester-linked liposomes, archaeosomes exhibit high pH and thermal stability. As adjuvants, can induce robust, long-lasting humoral cell-mediated immune responses enhance protection in murine models infectious disease cancer. constituted with total polar (TPL) archaea relatively complex, comprising >10 different lipid compounds. be semi-synthetic glycerolipids built on ether-linked isoprenoid...
In humans, the occurrence of prenatal exposure to ethanol is difficult validate objectively. Increased concentration fatty acid ethyl esters (FAEE) in meconium newborn may be a biomarker exposure. The validity this proposed was tested pregnant guinea pigs that received chronic oral administration 4 g ethanol/kg maternal body weight/day (n=8), isocaloric-sucrose/pair-feeding (n=8) or water (n=2) throughout gestation. At gestational day 65 (term, 66 69), each dam and her offspring were...
Glioma is the most common and difficult to treat brain cancer. Despite many efforts treatment, efficacy remains low. As neurosurgical removal standard procedure for glioma, a method, allowing both early detection exact determination of location, size extent tumor, could improve patient's positive response therapy.We propose application susceptibility weighted molecular magnetic resonance imaging using, targeted contrast agents, based on superparamagnetic iron oxide nanoparticles, the, glioma...
Wistar rats were randomly divided into groups of varying iodide intake: normal iodide; 10 times high and 100 on Days 7, 14, 28. Insignificant changes observed in thyroid hormone levels ( p > 0.05). Urinary iodine concentration content the glands increased after consumption from NI to HI < The urinary group 28 was 60–80 that group. mitochondrial superoxide production expressions Nrf2, Srx, Prx 3 all significantly increased, while Keap 1 decreased when compared or Immunofluorescence...
The increasing prevalence of the metabolic syndrome has made it a medical issue that currently affects 1 in 5 Canadians. is defined by risk factors predispose an individual to diabetes and cardiovascular disease. Current forms interventions have been inadequate as substantiated fact not reduced over years. objective this study was investigate therapeutic benefits novel microencapsulated probiotic blend treating syndrome. Three strains were into alginate-polylysine-alginate (APA)...
Mental Workload (MWL) is traditionally evaluated by psychophysiological signals using spectral analysis and event-related potentials. Robot-assisted Surgery (RAS) a complex task that involves human-robot interaction, multitasking, quick appropriate reactions to various stimuli unforeseen circumstances, as well frequent switches between surgical subtasks. There lack of standardized methodology for objectively monitoring surgeon's MWL during RAS. In this study, we propose an innovative...
Aims: To compare the incorporation rate (ICR) of fatty acid ethyl esters (FAEE) in hair between guinea pigs and humans, to assess relationship ethanol exposure FAEE concentrations hair. Methods: Published data from pregnant pigs, including maximum blood concentration (BEC), dosage regimen, total concentration, were compared with published alcoholic patients, where dose consumed reported. Mean values Vmax for humans obtained (26.2 24 mg/dl/h, respectively). Results: Total individual ICRs,...