A5077397108- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Tendon Structure and Treatment
- Total Knee Arthroplasty Outcomes
- Mesenchymal stem cell research
- Spine and Intervertebral Disc Pathology
- Bone health and osteoporosis research
- Cell Adhesion Molecules Research
- 3D Printing in Biomedical Research
- Musculoskeletal pain and rehabilitation
- Wound Healing and Treatments
- Bone fractures and treatments
- Orthopaedic implants and arthroplasty
- Proteoglycans and glycosaminoglycans research
- Pain Mechanisms and Treatments
- Veterinary Orthopedics and Neurology
- Bone health and treatments
- Periodontal Regeneration and Treatments
- Orthopedic Surgery and Rehabilitation
- Veterinary Equine Medical Research
- Lower Extremity Biomechanics and Pathologies
- Shoulder Injury and Treatment
- Molecular Biology Techniques and Applications
- TGF-β signaling in diseases
- Dermatologic Treatments and Research
University of Calgary
2015-2024
Alberta Bone and Joint Health Institute
2013-2024
AO Foundation
2022
University of Pennsylvania
2022
Tokai University
2022
Riverside Methodist Hospital
2014
Institute of Nuclear Physics, Polish Academy of Sciences
2011
National Research Council Canada
2011
University of the Assumption
2011
University of Ottawa
2011
Adult mammalian skin wounds heal by forming fibrotic scars. We report that full-thickness injuries of reindeer antler (velvet) regenerate, whereas back forms scar. Single-cell multi-omics reveal uninjured velvet fibroblasts resemble human fetal fibroblasts, express inflammatory mediators mimicking pro-fibrotic adult and rodent fibroblasts. Consequently, injury elicits site-specific immune responses: amplify myeloid infiltration maturation during repair, adopt an immunosuppressive phenotype...
Abstract The nuclei pulposi of the intervertebral discs (IVDs) contain a mixed population cell types at various stages maturation. This tissue is formed either by or with help cells from embryonic notochord, which appear to be replaced during development chondrocyte‐like uncertain origin. However, this transition occurs widely varying times, depending upon species – even breed animal being examined. There considerable debate among spine researchers as whether presence these residual...
A mouse model of glioblastoma multiforme was used to determine the accumulation a targeted contrast agent in tumor vessels.The agent, consisting superparamagnetic iron oxide coated with dextran, functionalized an anti-insulin-like-growth-factor binding protein 7 (anti-IGFBP7) single domain antibody.The near infrared marker, Cy5.5, also attached for vivo fluorescence study.A 9.4T magnetic resonance imaging (MRI) system studies on days 10 and 11 following inoculation.T 2 relaxation time...
Abstract Controversy remains whether articular cartilage has an endogenous stem/progenitor cell population, since its poor healing capacity after injury can lead to diseases such as osteoarthritis. In the joint environment there are mesenchymal cells (MSCs) in synovial membrane and fluid that differentiate into cartilage, but it is still under debate if these contribute repair vivo . this study, we isolated a Sca-1 positive, chondrogenesis capable population of mouse MSCs from C57BL6 MRL/MpJ...
A new therapeutic option for treating arthritis pain.
Abstract The three‐dimensional architecture of cells in the annulus fibrosus was studied by a systematic, histological examination using antibodies to cytoskeletal components, conjunction with confocal microscopy. Variations cell shape, arrangement cellular processes and were found both within between defined zones outer inner annulus. morphology three, novel is described: extended cordlike that form an interconnected network at periphery disc; extensive, sinuous region fibrosus; broad,...
Abstract Objective To study 3 body fluids for changes in the levels of 5 biomarkers cartilage metabolism during early phases experimental osteoarthritis (OA). Methods Twenty skeletally mature mixed‐breed canines underwent unilateral surgical transection anterior cruciate ligament. Samples joint fluid, serum, and urine were obtained preoperatively just before necropsy (3 weeks or 12 postoperatively). Biomarkers included 2 markers matrix synthesis/turnover (aggrecan 846 epitope C‐propeptide...
Abstract Background The aim of the current study was to evaluate innervation acetabular labrum in various zones and understand its potential role nociception proprioception hips with labral pathology. Methods A total twenty hip labrums were tagged excised intraoperatively from patients undergoing a replacement. After preparation, specimens cut thickness 10 μm divided into four quadrants (zones) using clock face pattern. Neurosensory structure distribution then evaluated Hematoxylin Eosin (H...
Abstract Cells from the nucleus pulposus of young (< 2 years) and old (> 5 non‐chondrodystrophoid dogs were studied using routine histology, confocal laser scanning microscopy transmission electron microscopy. The architecture cell structures – tissue scale down to subcellular was reported. Clusters notochordal cells observed in nuclei pulposi, ranging 10 426 each. These clusters resisted mechanical disruption showed evidence cell–cell signalling via gap junctions. (30–40 µm diameter)...
The effects of motion and increased levels stress on the biomechanical, biochemical, morphological properties healing medial collateral ligaments were assessed in a rabbit model. In one group, ligament left hindlimb was transected allowed to heal with cage activity for either 6 or 12 weeks. another permitted 4 weeks then placed under by inserting stainless steel pin perpendicularly underneath ligament. animals an additional 2 8 varus-valgus joint laxity stress-strain substance obtained....
In situ investigation of collagen and cell mechanics in bovine caudal discs using novel techniques confocal microscopy.To measure simultaneously the intercellular matrix inner outer anulus fibrosus intervertebral disc subjected to flexion.Mechanobiology studies, both vivo vitro, clearly demonstrate that mechanical factors can influence metabolic activity cells, altering expression key extracellular molecules. Essential elucidating mechanotransduction mechanisms is a detailed understanding...
Osteoarthritis (OA) is a debilitating and painful disease, the incidence of which increases with advancing age. One confounding aspects OA that there disconnect between severity joint degeneration intensity pain reported. This study examined relationship age, nociception, pathology in an animal model naturally occurring OA. Dunkin Hartley guinea pigs were grouped according to age: young (2-5 months) senescent (17-37 months). Joint nociception was objectively measured these animals by...
Abstract The development of new analgesic drugs has been hampered by the inability to translate preclinical findings humans. This failure is due in part weak connection between commonly used pain outcome measures rodents and clinical symptoms chronic pain. Most rodent studies rely on use experimenter-evoked assess behavior under ethologically unnatural conditions, which limits translational potential research. Here, we addressed this problem conducting an unbiased, prospective study...
Abstract Objective . To quantify the gene expression of aggrecan core protein and type II collagen in an experimental animal model osteoarthritis (OA). Methods Total RNA was extracted from articular cartilage unoperated knee joints OA produced by anterior cruciate ligament transection. The relative amounts messenger (mRNA) were evaluated Northern blot analysis. Results elevated 2.5 times, mRNA 2 8 knees compared to controls. Conclusion Chondrocytes are activated metabolically response joint...
The present study measured early-stage adaptation of bone mineral (BMD) in the periarticular cancellous canine knee (stifle) joint after anterior cruciate ligament (ACL) transection (ACLX). Regional changes BMD tibia and femur were analyzed by using quantitative computed tomography (qCT) at 3 wk 12 unilateral ACLX to determine whether there focal points for these occurred early induced injury. decreased rapidly ACLX, more pronounced response was femur. In 3-wk group, decreases femur,...
Complete ulnar collateral ligament tears incurred during the period fall 1977 through spring 1979 were treated under regional anesthesia by a single group of surgeons in an identical fashion as outpatients. Of 123 thumbs repaired, 69 (59%) available for follow-up examination. There 34 women and 35 men with average age 34.5 years (range, 16-61 years). The averaged 31.6 months 16-46 months). Weakness pinch was described none or mild 66 (96%) significant only three (4%). Stiffness (96%),...
The use of embryonic stem cells (ESCs) for regenerative medicine has generated increased attention due to the favorable attributes these cells; namely, they are pluripotent and possess long-term self-renewal capacity. initial aims present study were: (i) stirred suspension bioreactors expand differentiate ESCs into osteogenic chondrogenic cell types (ii) explore if ESC-derived influenced skeletal healing in an vivo fracture model. We show that differentiation protocols used static culture...