A5050627670- Cardiomyopathy and Myosin Studies
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- Cardiovascular Effects of Exercise
- Dialysis and Renal Disease Management
- Muscle and Compartmental Disorders
- Electrospun Nanofibers in Biomedical Applications
- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
- Nanoplatforms for cancer theranostics
- Graphene and Nanomaterials Applications
- Cardiac pacing and defibrillation studies
- Nanoparticles: synthesis and applications
- Electrolyte and hormonal disorders
- Radiopharmaceutical Chemistry and Applications
- Proteoglycans and glycosaminoglycans research
- Genomics and Rare Diseases
- Cardiovascular Function and Risk Factors
- Advanced Drug Delivery Systems
- Mesenchymal stem cell research
Yale University
2021-2024
Columbia University
2018-2019
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have wide potential application in basic research, drug discovery, and regenerative medicine, but functional maturation remains challenging. Here, we present a method whereby of hiPSC-CMs can be accelerated by simultaneous physiological Ca2+ frequency-ramped electrical pacing culture. This combination produces positive force-frequency behavior, twitch kinetics, robust β-adrenergic response, improved handling, cardiac...
Hypertrophic cardiomyopathy (HCM) is an inherited disorder often caused by mutations to sarcomeric genes. Many different HCM-associated TPM1 have been identified but they vary in their degrees of severity, prevalence, and rate disease progression. The pathogenicity many variants detected the clinical population remains unknown. Our objective was employ a computational modeling pipeline assess one such variant unknown significance, S215L, validate predictions using experimental methods....
The synovium envelops the diarthrodial joint and plays a key regulatory role in defining composition of synovial fluid through filtration biosynthesis critical boundary lubricants. Synovium changes often precede cartilage damage osteoarthritis. We describe novel
Hypertrophic and dilated cardiomyopathies (HCM DCM, respectively) are inherited disorders that may be caused by mutations to the same sarcomeric protein but have completely different clinical phenotypes. The precise mechanisms which point within gene bring about phenotypic diversity remain unclear. Our objective has been develop a mechanistic explanation of diverging phenotypes in two TPM1 mutations, E62Q (HCM) E54K (DCM). Drawing on data from literature experiments with stem cell-derived...
Introduction: Clonal hematopoiesis of indeterminate potential (CHIP) has been shown to be an independent risk factor for heart failure and is associated with increased cardiomyopathy mortality in cancer patients. Given that CHIP inflammatory cytokines, we sought characterize the secretome patients its impact on human engineered tissue (EHT) function. Methods: We used machine learning identify top cytokine features a cardio oncology cohort 173 Serum from breast who were age, co-morbidity...
Introduction: Cascade genetic screening for risk stratification is standard practice in the family of a patient diagnosed with hypertrophic cardiomyopathy (HCM), provided that pathogenic mutation present. We sought an alternative to testing cases where lacks any suspicious variants (genotype-negative). Hypothesis: Induced pluripotent stem cell (iPSC)-derived cardiomyocytes from HCM patients mutations exhibit one or more altered contractile parameters when formed into engineered heart tissues...
Missense mutations in alpha-tropomyosin (TPM1) can lead to development of hypertrophic (HCM) or dilated cardiomyopathy (DCM). HCM mutation E62Q and DCM E54K have previously been studied extensively experimental systems ranging from vitro biochemical assays animal models, although some conflicting results found. We undertook a detailed multi-scale assessment these mutants that included atomistic simulations, regulated motility (IVM) assays, finally physiologically relevant human engineered...