A5064655594- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Solar and Space Plasma Dynamics
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Methane Hydrates and Related Phenomena
- Cell Adhesion Molecules Research
- Photoacoustic and Ultrasonic Imaging
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Earthquake Detection and Analysis
- Microfluidic and Bio-sensing Technologies
- Optical Imaging and Spectroscopy Techniques
- Sepsis Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- T-cell and B-cell Immunology
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Respiratory Support and Mechanisms
- Digital Holography and Microscopy
- Esophageal Cancer Research and Treatment
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Cancer, Hypoxia, and Metabolism
Environmental Molecular Sciences Laboratory
2023
Cornell University
2020-2023
Korea Advanced Institute of Science and Technology
2013-2021
Center for NanoScience
2015
Kootenay Association for Science & Technology
2013
Agency for Science, Technology and Research
2010
Institute of Microelectronics
2010
Yale University
2010
The lung is highly vulnerable during sepsis, yet its functional deterioration accompanied by disturbances in the pulmonary microcirculation poorly understood. This study aimed to investigate how distorted sepsis-induced acute injury (ALI) and reveal underlying cellular pathophysiologic mechanism. Using a custom-made intravital microscopic imaging system murine model of ALI, we achieved direct real-time visualisation circulating cells vivo . We derived capillary ratio (FCR) as quantitative...
Lacteals are lymphatic vessels located at the center of each intestinal villus and provide essential transport routes for lipids other lipophilic molecules. However, it is unclear how absorbed molecules transported through lacteal. Here, we used reporter mice that express GFP under control lymphatic-specific promoter Prox1 a custom-built confocal microscope performed intravital real-time visualization absorption dynamics fluorescence-tagged fatty acids (FAs) various exogenous in villi vivo....
Abstract Emerging evidence suggests that intestinal stromal cells (IntSCs) play essential roles in maintaining homeostasis. However, the extent of heterogeneity within villi compartment and how IntSCs regulate structure function specialized lymphatic capillary called lacteal remain elusive. Here we show selective hyperactivation or depletion YAP/TAZ PDGFRβ + leads to sprouting regression with junctional disintegration impaired dietary fat uptake. Indeed, mechanical osmotic stress regulates...
Signal transducer and activator of transcription 3 (STAT3) is constitutively activated in psoriatic skin inflammation acts as a key player the pathogenesis progression this autoimmune disease. Although numerous inhibitors that intervene STAT3-associated pathways have been tested, an effective, highly specific inhibitor STAT3 has yet to be identified. Here, we evaluated vitro vivo biological activity therapeutic efficacy high-affinity peptide for (APTstat3) after topical treatment via...
Abstract Proper storage of excessive dietary fat into subcutaneous adipose tissue (SAT) prevents ectopic lipid deposition-induced insulin resistance, yet the underlying mechanism remains unclear. Here, we identify angiopoietin-2 (Angpt2)–integrin α5β1 signaling as an inducer uptake specifically in SAT. Adipocyte-specific deletion Angpt2 markedly reduced fatty acid and SAT, leading to accumulation glucose-consuming organs including skeletal muscle liver systemic resistance. Mechanistically,...
The number of circulating tumor cell (CTC) in the peripheral blood cancer patients can be a valuable biomarker for diagnosis and treatment monitoring. In this study, we implemented custom-design video-rate confocal microscopy system capable direct visualization fast flowing CTC at great saphenous vein (GSV) live animal model vivo. Continuous acquisition images GSV revealed highly dynamic time-dependent changes intravenously injected cells. By extracting calibration factor through...
Abstract Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging in vivo have been proposed, most require the use labelling and also provide only qualitative information. Holographic approach based on measuring refractive index distributions cells, however, circumvent these problems offer quantitative label-free capability. Here, we demonstrate two-...
Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role vascular barrier various pathological conditions.Despite its importance as marker cell damage and impairment system, vivo visualization ESL has remained challenging technical issue.In this work, we implemented system integrated custom-design video-rate laser scanning confocal microscopy platform.Using real-time cellular-level microscopic lung was...
Lymph node (LN) is an important immune organ that controls adaptive responses against foreign pathogens and abnormal cells. To facilitate efficient function, LN has highly organized 3D cellular structures, vascular lymphatic system. Unfortunately, conventional histological analysis relying on thin-sliced tissue limitations in due to structural disruption loss the processes of fixation slicing. Optical sectioning confocal microscopy been utilized analyze structure intact without physical...
Lymph nodes (LN) are major checkpoints for circulating T lymphocytes to recognize foreign antigens collected from peripheral tissue. High endothelial venule (HEV) in LN facilitates the effective transmigration of blood into LN. There have been many efforts visualize trafficking across HEV understand underlying mechanism. However, due insufficient spatiotemporal resolution and lack an vivo labeling method, clear visualization dynamic behaviors rapidly flowing their difficult. In this work, we...
Abstract Thyroid gland vasculature has a distinguishable characteristic of endothelial fenestrae, critical component for proper molecular transport. However, the signaling pathway that critically governs maintenance thyroid vascular integrity, including is poorly understood. Here, we found profound and distinct expression follicular epithelial VEGF ‐A VEGFR 2 were precisely regulated by circulating thyrotropin, while there no meaningful angiopoietin–Tie2 system in gland. Our genetic...
Indocyanine green (ICG) is a near-infrared fluorophore approved for human use which has been widely used various clinical applications. Despite the well-established usage, our understanding about microscopic in vivo pharmacokinetics of systemically administered ICG relatively limited. In this work, we successfully visualized real-time pharmacokinetic dynamics intravenously injected free-form and liposomal cellular resolution by utilizing custom-built video-rate near infrared laser-scanning...
Bone marrow is a vital tissue that produces the majority of erythrocytes, thrombocytes, and immune cells. transplantation (BMT) has been widely performed in patients with blood disorders cancers. However, cellular-level behaviors transplanted bone cells over wide-areas host after BMT are not fully understood yet. In this work, we longitudinal wide-area observation calvarial vivo. Using H2B-GFP/β-actin-DsRed double-transgenic mouse model as donor, subcellular-level nuclear-cytoplasmic...
Visualization of cellular dynamics in the gastrointestinal tract living mouse model to investigate pathophysiology has been a long-pursuing goal. Especially, for chronic disease such as Crohn's disease, longitudinal observation luminal surface small intestine single is highly desirable complex pathogenesis sequential time points. In this work, by utilizing micro-GRIN lens based side-view endomicroscope integrated into video-rate confocal microscopy system, we successfully performed...
High endothelial venules (HEVs) effectively recruit circulating lymphocytes from the blood to lymph nodes. HEVs have cells (ECs) and perivascular sheaths consisting of fibroblastic reticular (FRCs). Yet, post-luminal lymphocyte migration steps are not well elucidated. Herein, we performed intravital imaging investigate T- B-cell in popliteal node, trans-EC migration, crawling channel (a narrow space between ECs FRCs) trans-FRC migration. The T occurred a PNAd-dependent manner. Remarkably,...
Transdermal skin delivery is a method to transport various topical formulations deeper layer non-invasively. Permeability analysis of many delivering agents has been mostly conducted by simple tape stripping method. However, it cannot reveal detailed depth-dependent distribution profile transdermally delivered in the skin. In this work, we achieved cellular-level depth-defined visualization fluorophore-labelled human epidermal growth factor (EGF) using encapsulation with common liposomes and...
Multiphoton microscopy techniques, such as two-photon (2PM) and three-photon (3PM), are powerful tools for deep-tissue in vivo imaging with subcellular resolution. 3PM has two major advantages over 2PM that been widely used biology laboratories: (i) longer attenuation length scattering tissues by employing ~1,300 nm or ~1,700 excitation laser; (ii) less background fluorescence generation due to higher-order nonlinear excitation. As a result, allows high-contrast structural functional deep...
A 1.8-mW, 18.5-mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> 64-channel current readout ASIC was implemented in 0.18-μm CMOS together with a new calibration scheme for silicon nanowire biosensor arrays. The consists of 64 channels dedicated and conditioning circuits which incorporate correlated double sampling to reduce the effect 1/f noise offset from analog front-end. provides 10-bit digital output rate 300 S/s whilst achieving...
In vivo, longitudinal observation of tumorigenesis in a live mouse model over an extended time period has been actively pursued to obtain better understanding the cellular and molecular mechanism highly complex tumor microenvironment. However, common intravital imaging approaches based on conventional laser scanning confocal or two-photon microscope have mostly limited superficial parts solid tissue. this work, we implemented small diameter needle-shaped side-view endomicroscope that can be...
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging in vivo have been proposed, most require the use labelling and also provide only qualitative information. Holographic approach based on measuring refractive index distributions cells, however, circumvent these problems offer quantitative label-free capability. Here, we demonstrate two- three-dimensional...
By utilizing custom-design high-speed video-rate laser-scanning confocal microscope system for visualizing fast-flowing cells at the great saphenous vein of mouse, we quantitated circulating tumor whole body blood, in vivo, by hemocytometric imaging analysis.
Multiphoton microscopy techniques, such as two-photon (2PM) and three-photon (3PM), are powerful tools for deep-tissue in vivo imaging with subcellular resolution. 3PM has two major advantages over 2PM that been widely used biology laboratories: (i) longer attenuation length scattering tissues by employing ~1,300 nm or ~1,700 excitation laser; (ii) less background fluorescence generation due to higher-order nonlinear excitation. As a result, allows high-contrast structural functional deep...